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Optimising genetic transformation of Trypanosoma cruzi using hydroxyurea-induced cell-cycle synchronisation

The limited flexibility and time-consuming nature of the genetic manipulation procedures applicable to Trypanosoma cruzi continue to restrict the functional dissection of this parasite. We hypothesised that transformation efficiency could be enhanced if electroporation was timed to coincide with DNA...

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Detalles Bibliográficos
Autores principales:	Olmo, Francisco, Costa, Fernanda C., Mann, Gurdip Singh, Taylor, Martin C., Kelly, John M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier/North-Holland Biomedical Press 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254250/ https://www.ncbi.nlm.nih.gov/pubmed/29990513 http://dx.doi.org/10.1016/j.molbiopara.2018.07.002

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