Cargando…
2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
BACKGROUND: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line-associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254312/ http://dx.doi.org/10.1093/ofid/ofy210.1746 |
_version_ | 1783373694435852288 |
---|---|
author | Green, Julianne Junkins, Alan Bryant, Kristina Stout, Gordon Woods, Charles |
author_facet | Green, Julianne Junkins, Alan Bryant, Kristina Stout, Gordon Woods, Charles |
author_sort | Green, Julianne |
collection | PubMed |
description | BACKGROUND: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line-associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless access devices (NADs) to reduce CLABSIs (Figure 1). These port protectors achieve ≥4-log reduction in colony-forming units (CFU’s) of S. aureus, S. epidermidis, E. coli, C. albicans, P. aeruginosa and C. glabrata. The effect against F. oxysporon has not been reported. METHODS: F. oxysporon was grown on Sabouraud Dextrose (Sab Dex) agar. Two protocols were used: (1) aliquots of ~1 × 10(6) CFU were loaded to the surface of eight NAD’s and allowed to air dry, and (2) the surface of 20 NAD’s were contaminated via touching to a dense lawn of Fusarium on culture plates. Half of the NAD’s were decontaminated with a port protector for one minute; the other half had no decontamination step (along with positive/negative controls). NAD’s were then (1) placed whole in Sab Dex broth or (2) touched to a fresh Sab Dex agar plate, and growth observed for 7 days. RESULTS: In all cases, NAD’s that had been decontaminated with the alcohol-impregnated port protector showed no growth after seven days in broth (Figure 2) or on plates (Figure 3). NAD’s lacking the decontamination step invariably showed abundant growth. CONCLUSION: Use of two different techniques demonstrates that a 70% isopropyl alcohol impregnated port protector achieves decontamination of F. oxysporon from the surface of needleless access devices. DISCLOSURES: All authors: No reported disclosures. |
format | Online Article Text |
id | pubmed-6254312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62543122018-11-28 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector Green, Julianne Junkins, Alan Bryant, Kristina Stout, Gordon Woods, Charles Open Forum Infect Dis Abstracts BACKGROUND: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line-associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless access devices (NADs) to reduce CLABSIs (Figure 1). These port protectors achieve ≥4-log reduction in colony-forming units (CFU’s) of S. aureus, S. epidermidis, E. coli, C. albicans, P. aeruginosa and C. glabrata. The effect against F. oxysporon has not been reported. METHODS: F. oxysporon was grown on Sabouraud Dextrose (Sab Dex) agar. Two protocols were used: (1) aliquots of ~1 × 10(6) CFU were loaded to the surface of eight NAD’s and allowed to air dry, and (2) the surface of 20 NAD’s were contaminated via touching to a dense lawn of Fusarium on culture plates. Half of the NAD’s were decontaminated with a port protector for one minute; the other half had no decontamination step (along with positive/negative controls). NAD’s were then (1) placed whole in Sab Dex broth or (2) touched to a fresh Sab Dex agar plate, and growth observed for 7 days. RESULTS: In all cases, NAD’s that had been decontaminated with the alcohol-impregnated port protector showed no growth after seven days in broth (Figure 2) or on plates (Figure 3). NAD’s lacking the decontamination step invariably showed abundant growth. CONCLUSION: Use of two different techniques demonstrates that a 70% isopropyl alcohol impregnated port protector achieves decontamination of F. oxysporon from the surface of needleless access devices. DISCLOSURES: All authors: No reported disclosures. Oxford University Press 2018-11-26 /pmc/articles/PMC6254312/ http://dx.doi.org/10.1093/ofid/ofy210.1746 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Abstracts Green, Julianne Junkins, Alan Bryant, Kristina Stout, Gordon Woods, Charles 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector |
title | 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector |
title_full | 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector |
title_fullStr | 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector |
title_full_unstemmed | 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector |
title_short | 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector |
title_sort | 2090. decontamination of fusarium oxysporon from a central line needleless access device using a 70% isopropyl alcohol impregnated port protector |
topic | Abstracts |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254312/ http://dx.doi.org/10.1093/ofid/ofy210.1746 |
work_keys_str_mv | AT greenjulianne 2090decontaminationoffusariumoxysporonfromacentrallineneedlelessaccessdeviceusinga70isopropylalcoholimpregnatedportprotector AT junkinsalan 2090decontaminationoffusariumoxysporonfromacentrallineneedlelessaccessdeviceusinga70isopropylalcoholimpregnatedportprotector AT bryantkristina 2090decontaminationoffusariumoxysporonfromacentrallineneedlelessaccessdeviceusinga70isopropylalcoholimpregnatedportprotector AT stoutgordon 2090decontaminationoffusariumoxysporonfromacentrallineneedlelessaccessdeviceusinga70isopropylalcoholimpregnatedportprotector AT woodscharles 2090decontaminationoffusariumoxysporonfromacentrallineneedlelessaccessdeviceusinga70isopropylalcoholimpregnatedportprotector |