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2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector

BACKGROUND: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line-associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless...

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Autores principales: Green, Julianne, Junkins, Alan, Bryant, Kristina, Stout, Gordon, Woods, Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254312/
http://dx.doi.org/10.1093/ofid/ofy210.1746
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author Green, Julianne
Junkins, Alan
Bryant, Kristina
Stout, Gordon
Woods, Charles
author_facet Green, Julianne
Junkins, Alan
Bryant, Kristina
Stout, Gordon
Woods, Charles
author_sort Green, Julianne
collection PubMed
description BACKGROUND: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line-associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless access devices (NADs) to reduce CLABSIs (Figure 1). These port protectors achieve ≥4-log reduction in colony-forming units (CFU’s) of S. aureus, S. epidermidis, E. coli, C. albicans, P. aeruginosa and C. glabrata. The effect against F. oxysporon has not been reported. METHODS: F. oxysporon was grown on Sabouraud Dextrose (Sab Dex) agar. Two protocols were used: (1) aliquots of ~1 × 10(6) CFU were loaded to the surface of eight NAD’s and allowed to air dry, and (2) the surface of 20 NAD’s were contaminated via touching to a dense lawn of Fusarium on culture plates. Half of the NAD’s were decontaminated with a port protector for one minute; the other half had no decontamination step (along with positive/negative controls). NAD’s were then (1) placed whole in Sab Dex broth or (2) touched to a fresh Sab Dex agar plate, and growth observed for 7 days. RESULTS: In all cases, NAD’s that had been decontaminated with the alcohol-impregnated port protector showed no growth after seven days in broth (Figure 2) or on plates (Figure 3). NAD’s lacking the decontamination step invariably showed abundant growth. CONCLUSION: Use of two different techniques demonstrates that a 70% isopropyl alcohol impregnated port protector achieves decontamination of F. oxysporon from the surface of needleless access devices. DISCLOSURES: All authors: No reported disclosures.
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spelling pubmed-62543122018-11-28 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector Green, Julianne Junkins, Alan Bryant, Kristina Stout, Gordon Woods, Charles Open Forum Infect Dis Abstracts BACKGROUND: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line-associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless access devices (NADs) to reduce CLABSIs (Figure 1). These port protectors achieve ≥4-log reduction in colony-forming units (CFU’s) of S. aureus, S. epidermidis, E. coli, C. albicans, P. aeruginosa and C. glabrata. The effect against F. oxysporon has not been reported. METHODS: F. oxysporon was grown on Sabouraud Dextrose (Sab Dex) agar. Two protocols were used: (1) aliquots of ~1 × 10(6) CFU were loaded to the surface of eight NAD’s and allowed to air dry, and (2) the surface of 20 NAD’s were contaminated via touching to a dense lawn of Fusarium on culture plates. Half of the NAD’s were decontaminated with a port protector for one minute; the other half had no decontamination step (along with positive/negative controls). NAD’s were then (1) placed whole in Sab Dex broth or (2) touched to a fresh Sab Dex agar plate, and growth observed for 7 days. RESULTS: In all cases, NAD’s that had been decontaminated with the alcohol-impregnated port protector showed no growth after seven days in broth (Figure 2) or on plates (Figure 3). NAD’s lacking the decontamination step invariably showed abundant growth. CONCLUSION: Use of two different techniques demonstrates that a 70% isopropyl alcohol impregnated port protector achieves decontamination of F. oxysporon from the surface of needleless access devices. DISCLOSURES: All authors: No reported disclosures. Oxford University Press 2018-11-26 /pmc/articles/PMC6254312/ http://dx.doi.org/10.1093/ofid/ofy210.1746 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Abstracts
Green, Julianne
Junkins, Alan
Bryant, Kristina
Stout, Gordon
Woods, Charles
2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
title 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
title_full 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
title_fullStr 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
title_full_unstemmed 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
title_short 2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device Using a 70% Isopropyl Alcohol Impregnated Port Protector
title_sort 2090. decontamination of fusarium oxysporon from a central line needleless access device using a 70% isopropyl alcohol impregnated port protector
topic Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254312/
http://dx.doi.org/10.1093/ofid/ofy210.1746
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