Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expre...

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Detalles Bibliográficos
Autores principales: Ruan, Chang-Chun, Zhang, Hao, Zhang, Lian-Xue, Liu, Zhi, Sun, Guang-Zhi, Lei, Jun, Qin, Yu-Xia, Zheng, Yi-Nan, Li, Xiang, Pan, Hong-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2009
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254368/
https://www.ncbi.nlm.nih.gov/pubmed/19513004
http://dx.doi.org/10.3390/molecules14062043
Descripción
Sumario:An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh(1).

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