Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expre...

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Autores principales: Ruan, Chang-Chun, Zhang, Hao, Zhang, Lian-Xue, Liu, Zhi, Sun, Guang-Zhi, Lei, Jun, Qin, Yu-Xia, Zheng, Yi-Nan, Li, Xiang, Pan, Hong-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2009
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254368/
https://www.ncbi.nlm.nih.gov/pubmed/19513004
http://dx.doi.org/10.3390/molecules14062043
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author Ruan, Chang-Chun
Zhang, Hao
Zhang, Lian-Xue
Liu, Zhi
Sun, Guang-Zhi
Lei, Jun
Qin, Yu-Xia
Zheng, Yi-Nan
Li, Xiang
Pan, Hong-Yu
author_facet Ruan, Chang-Chun
Zhang, Hao
Zhang, Lian-Xue
Liu, Zhi
Sun, Guang-Zhi
Lei, Jun
Qin, Yu-Xia
Zheng, Yi-Nan
Li, Xiang
Pan, Hong-Yu
author_sort Ruan, Chang-Chun
collection PubMed
description An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh(1).
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spelling pubmed-62543682018-11-30 Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase Ruan, Chang-Chun Zhang, Hao Zhang, Lian-Xue Liu, Zhi Sun, Guang-Zhi Lei, Jun Qin, Yu-Xia Zheng, Yi-Nan Li, Xiang Pan, Hong-Yu Molecules Communication An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh(1). Molecular Diversity Preservation International 2009-06-08 /pmc/articles/PMC6254368/ /pubmed/19513004 http://dx.doi.org/10.3390/molecules14062043 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Ruan, Chang-Chun
Zhang, Hao
Zhang, Lian-Xue
Liu, Zhi
Sun, Guang-Zhi
Lei, Jun
Qin, Yu-Xia
Zheng, Yi-Nan
Li, Xiang
Pan, Hong-Yu
Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase
title Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase
title_full Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase
title_fullStr Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase
title_full_unstemmed Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase
title_short Biotransformation of Ginsenoside Rf to Rh(1) by Recombinant β-Glucosidase
title_sort biotransformation of ginsenoside rf to rh(1) by recombinant β-glucosidase
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254368/
https://www.ncbi.nlm.nih.gov/pubmed/19513004
http://dx.doi.org/10.3390/molecules14062043
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