In silico analysis of transferable QepA variants and related chromosomal efflux pumps

OBJECTIVES: The present study aimed to detect the presence of undescribed QepA variants in GenBank records. MATERIAL AND METHODS: The DNA and amino acid sequences of QepA1 were compared with what is present in GenBank. Only annealings with a >80% identity were considered. No synthetic or partial...

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Detalles Bibliográficos
Autor principal: Ruiz, Joaquim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedad Española de Quimioterapia 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254477/
https://www.ncbi.nlm.nih.gov/pubmed/30378393
Descripción
Sumario:OBJECTIVES: The present study aimed to detect the presence of undescribed QepA variants in GenBank records. MATERIAL AND METHODS: The DNA and amino acid sequences of QepA1 were compared with what is present in GenBank. Only annealings with a >80% identity were considered. No synthetic or partial sequences were included in the analyses. RESULTS: The results showed the presence of 10 different QepA variants, 6 of them which were previously non-designated as specific allelic variants. In addition, high identity levels with chromosomal MSF efflux pumps belonging to microorganisms of the Pseudorhodoferax genus and other Comamonadaceae were detected. CONCLUSIONS: The presence of undescribed QepA variants in GenBank is reported and the presence of related sequences among members of Burkholderiales order is described.

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