In silico analysis of transferable QepA variants and related chromosomal efflux pumps

OBJECTIVES: The present study aimed to detect the presence of undescribed QepA variants in GenBank records. MATERIAL AND METHODS: The DNA and amino acid sequences of QepA1 were compared with what is present in GenBank. Only annealings with a >80% identity were considered. No synthetic or partial...

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Autor principal: Ruiz, Joaquim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedad Española de Quimioterapia 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254477/
https://www.ncbi.nlm.nih.gov/pubmed/30378393
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author Ruiz, Joaquim
author_facet Ruiz, Joaquim
author_sort Ruiz, Joaquim
collection PubMed
description OBJECTIVES: The present study aimed to detect the presence of undescribed QepA variants in GenBank records. MATERIAL AND METHODS: The DNA and amino acid sequences of QepA1 were compared with what is present in GenBank. Only annealings with a >80% identity were considered. No synthetic or partial sequences were included in the analyses. RESULTS: The results showed the presence of 10 different QepA variants, 6 of them which were previously non-designated as specific allelic variants. In addition, high identity levels with chromosomal MSF efflux pumps belonging to microorganisms of the Pseudorhodoferax genus and other Comamonadaceae were detected. CONCLUSIONS: The presence of undescribed QepA variants in GenBank is reported and the presence of related sequences among members of Burkholderiales order is described.
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spelling pubmed-62544772018-12-11 In silico analysis of transferable QepA variants and related chromosomal efflux pumps Ruiz, Joaquim Rev Esp Quimioter Brief Report OBJECTIVES: The present study aimed to detect the presence of undescribed QepA variants in GenBank records. MATERIAL AND METHODS: The DNA and amino acid sequences of QepA1 were compared with what is present in GenBank. Only annealings with a >80% identity were considered. No synthetic or partial sequences were included in the analyses. RESULTS: The results showed the presence of 10 different QepA variants, 6 of them which were previously non-designated as specific allelic variants. In addition, high identity levels with chromosomal MSF efflux pumps belonging to microorganisms of the Pseudorhodoferax genus and other Comamonadaceae were detected. CONCLUSIONS: The presence of undescribed QepA variants in GenBank is reported and the presence of related sequences among members of Burkholderiales order is described. Sociedad Española de Quimioterapia 2018-11-23 2018-12 /pmc/articles/PMC6254477/ /pubmed/30378393 Text en © The Author 2018 https://creativecommons.org/licenses/by-nc/4.0/ The article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Brief Report
Ruiz, Joaquim
In silico analysis of transferable QepA variants and related chromosomal efflux pumps
title In silico analysis of transferable QepA variants and related chromosomal efflux pumps
title_full In silico analysis of transferable QepA variants and related chromosomal efflux pumps
title_fullStr In silico analysis of transferable QepA variants and related chromosomal efflux pumps
title_full_unstemmed In silico analysis of transferable QepA variants and related chromosomal efflux pumps
title_short In silico analysis of transferable QepA variants and related chromosomal efflux pumps
title_sort in silico analysis of transferable qepa variants and related chromosomal efflux pumps
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254477/
https://www.ncbi.nlm.nih.gov/pubmed/30378393
work_keys_str_mv AT ruizjoaquim insilicoanalysisoftransferableqepavariantsandrelatedchromosomaleffluxpumps

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