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Cell tension and mechanical regulation of cell volume

Animal cells use an unknown mechanism to control their growth and physical size. Here, using the fluorescence exclusion method, we measure cell volume for adherent cells on substrates of varying stiffness. We discover that the cell volume has a complex dependence on substrate stiffness and is positi...

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Autores principales: Perez Gonzalez, Nicolas, Tao, Jiaxiang, Rochman, Nash D., Vig, Dhruv, Wirtz, Denis, Sun, Sean X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254581/
https://www.ncbi.nlm.nih.gov/pubmed/30113884
http://dx.doi.org/10.1091/mbc.E18-04-0213
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author Perez Gonzalez, Nicolas
Tao, Jiaxiang
Rochman, Nash D.
Vig, Dhruv
Wirtz, Denis
Sun, Sean X.
author_facet Perez Gonzalez, Nicolas
Tao, Jiaxiang
Rochman, Nash D.
Vig, Dhruv
Wirtz, Denis
Sun, Sean X.
author_sort Perez Gonzalez, Nicolas
collection PubMed
description Animal cells use an unknown mechanism to control their growth and physical size. Here, using the fluorescence exclusion method, we measure cell volume for adherent cells on substrates of varying stiffness. We discover that the cell volume has a complex dependence on substrate stiffness and is positively correlated with the size of the cell adhesion to the substrate. From a mechanical force–balance condition that determines the geometry of the cell surface, we find that the observed cell volume variation can be predicted quantitatively from the distribution of active myosin through the cell cortex. To connect cell mechanical tension with cell size homeostasis, we quantified the nuclear localization of YAP/TAZ, a transcription factor involved in cell growth and proliferation. We find that the level of nuclear YAP/TAZ is positively correlated with the average cell volume. Moreover, the level of nuclear YAP/TAZ is also connected to cell tension, as measured by the amount of phosphorylated myosin. Cells with greater apical tension tend to have higher levels of nuclear YAP/TAZ and a larger cell volume. These results point to a size-sensing mechanism based on mechanical tension: the cell tension increases as the cell grows, and increasing tension feeds back biochemically to growth and proliferation control.
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spelling pubmed-62545812019-02-04 Cell tension and mechanical regulation of cell volume Perez Gonzalez, Nicolas Tao, Jiaxiang Rochman, Nash D. Vig, Dhruv Wirtz, Denis Sun, Sean X. Mol Biol Cell Articles Animal cells use an unknown mechanism to control their growth and physical size. Here, using the fluorescence exclusion method, we measure cell volume for adherent cells on substrates of varying stiffness. We discover that the cell volume has a complex dependence on substrate stiffness and is positively correlated with the size of the cell adhesion to the substrate. From a mechanical force–balance condition that determines the geometry of the cell surface, we find that the observed cell volume variation can be predicted quantitatively from the distribution of active myosin through the cell cortex. To connect cell mechanical tension with cell size homeostasis, we quantified the nuclear localization of YAP/TAZ, a transcription factor involved in cell growth and proliferation. We find that the level of nuclear YAP/TAZ is positively correlated with the average cell volume. Moreover, the level of nuclear YAP/TAZ is also connected to cell tension, as measured by the amount of phosphorylated myosin. Cells with greater apical tension tend to have higher levels of nuclear YAP/TAZ and a larger cell volume. These results point to a size-sensing mechanism based on mechanical tension: the cell tension increases as the cell grows, and increasing tension feeds back biochemically to growth and proliferation control. The American Society for Cell Biology 2018-10-15 /pmc/articles/PMC6254581/ /pubmed/30113884 http://dx.doi.org/10.1091/mbc.E18-04-0213 Text en © 2018 Perez Gonzalez, Tao, Rochman, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Perez Gonzalez, Nicolas
Tao, Jiaxiang
Rochman, Nash D.
Vig, Dhruv
Wirtz, Denis
Sun, Sean X.
Cell tension and mechanical regulation of cell volume
title Cell tension and mechanical regulation of cell volume
title_full Cell tension and mechanical regulation of cell volume
title_fullStr Cell tension and mechanical regulation of cell volume
title_full_unstemmed Cell tension and mechanical regulation of cell volume
title_short Cell tension and mechanical regulation of cell volume
title_sort cell tension and mechanical regulation of cell volume
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254581/
https://www.ncbi.nlm.nih.gov/pubmed/30113884
http://dx.doi.org/10.1091/mbc.E18-04-0213
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