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Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans

This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Abo...

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Autores principales: Castillo, Alva, Rascón-Chu, Agustín, Vargas, Georgina, Carvajal-Millán, Elizabeth, Valenzuela-Soto, Elisa, Sotelo-Mundo, Rogerio R., Martínez, Ana Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254845/
https://www.ncbi.nlm.nih.gov/pubmed/19924054
http://dx.doi.org/10.3390/molecules14104159
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author Castillo, Alva
Rascón-Chu, Agustín
Vargas, Georgina
Carvajal-Millán, Elizabeth
Valenzuela-Soto, Elisa
Sotelo-Mundo, Rogerio R.
Martínez, Ana Luisa
author_facet Castillo, Alva
Rascón-Chu, Agustín
Vargas, Georgina
Carvajal-Millán, Elizabeth
Valenzuela-Soto, Elisa
Sotelo-Mundo, Rogerio R.
Martínez, Ana Luisa
author_sort Castillo, Alva
collection PubMed
description This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 μg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase.
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spelling pubmed-62548452018-11-30 Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans Castillo, Alva Rascón-Chu, Agustín Vargas, Georgina Carvajal-Millán, Elizabeth Valenzuela-Soto, Elisa Sotelo-Mundo, Rogerio R. Martínez, Ana Luisa Molecules Communication This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 μg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase. Molecular Diversity Preservation International 2009-10-16 /pmc/articles/PMC6254845/ /pubmed/19924054 http://dx.doi.org/10.3390/molecules14104159 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Castillo, Alva
Rascón-Chu, Agustín
Vargas, Georgina
Carvajal-Millán, Elizabeth
Valenzuela-Soto, Elisa
Sotelo-Mundo, Rogerio R.
Martínez, Ana Luisa
Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
title Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
title_full Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
title_fullStr Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
title_full_unstemmed Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
title_short Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
title_sort trametes sp. as a source of biopolymer cross-linking agents: laccase induced gelation of ferulated arabinoxylans
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254845/
https://www.ncbi.nlm.nih.gov/pubmed/19924054
http://dx.doi.org/10.3390/molecules14104159
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