Cargando…
Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans
This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Abo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254845/ https://www.ncbi.nlm.nih.gov/pubmed/19924054 http://dx.doi.org/10.3390/molecules14104159 |
_version_ | 1783373819190181888 |
---|---|
author | Castillo, Alva Rascón-Chu, Agustín Vargas, Georgina Carvajal-Millán, Elizabeth Valenzuela-Soto, Elisa Sotelo-Mundo, Rogerio R. Martínez, Ana Luisa |
author_facet | Castillo, Alva Rascón-Chu, Agustín Vargas, Georgina Carvajal-Millán, Elizabeth Valenzuela-Soto, Elisa Sotelo-Mundo, Rogerio R. Martínez, Ana Luisa |
author_sort | Castillo, Alva |
collection | PubMed |
description | This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 μg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase. |
format | Online Article Text |
id | pubmed-6254845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-62548452018-11-30 Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans Castillo, Alva Rascón-Chu, Agustín Vargas, Georgina Carvajal-Millán, Elizabeth Valenzuela-Soto, Elisa Sotelo-Mundo, Rogerio R. Martínez, Ana Luisa Molecules Communication This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 μg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase. Molecular Diversity Preservation International 2009-10-16 /pmc/articles/PMC6254845/ /pubmed/19924054 http://dx.doi.org/10.3390/molecules14104159 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Communication Castillo, Alva Rascón-Chu, Agustín Vargas, Georgina Carvajal-Millán, Elizabeth Valenzuela-Soto, Elisa Sotelo-Mundo, Rogerio R. Martínez, Ana Luisa Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans |
title | Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans |
title_full | Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans |
title_fullStr | Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans |
title_full_unstemmed | Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans |
title_short | Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans |
title_sort | trametes sp. as a source of biopolymer cross-linking agents: laccase induced gelation of ferulated arabinoxylans |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254845/ https://www.ncbi.nlm.nih.gov/pubmed/19924054 http://dx.doi.org/10.3390/molecules14104159 |
work_keys_str_mv | AT castilloalva trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans AT rasconchuagustin trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans AT vargasgeorgina trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans AT carvajalmillanelizabeth trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans AT valenzuelasotoelisa trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans AT sotelomundorogerior trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans AT martinezanaluisa trametesspasasourceofbiopolymercrosslinkingagentslaccaseinducedgelationofferulatedarabinoxylans |