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Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
BACKGROUND: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. PURPOSE: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze dry...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255055/ https://www.ncbi.nlm.nih.gov/pubmed/30538463 http://dx.doi.org/10.2147/IJN.S181855 |
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author | Yu, Fei Li, Ming Yuan, Zhipeng Rao, Feng Fang, Xingxing Jiang, Baoguo Wen, Yongqiang Zhang, Peixun |
author_facet | Yu, Fei Li, Ming Yuan, Zhipeng Rao, Feng Fang, Xingxing Jiang, Baoguo Wen, Yongqiang Zhang, Peixun |
author_sort | Yu, Fei |
collection | PubMed |
description | BACKGROUND: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. PURPOSE: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair. METHODS: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone. Postmodel specimens and micro computed tomography (CT) were used to observe any macroscopic morphological changes in the articular cartilage and subchondral bone, whereas multiple staining methods were used to observe all microcosmic morphological changes. Gross scores and Mankin scores were used to evaluate the repair condition. Immunohistochemical staining was employed to detect protein expression. RESULTS: When the repair included the resveratrol–PLA–gelatin porous nano-scaffold, the repaired cartilage and subchondral bone were in better condition. The expression levels of SIRT1, type II collagen, and PI3K/AKT signaling pathway-related proteins (AKT, VEGF, PTEN, Caspase 9, and MMP13) changed significantly. The expression levels of SIRT1,AKT and type II collagen proteins increased significantly, while the expression levels of VEGF, PTEN, Caspase9 and MMP13 proteins decreased significantly compared with the repair included blank porous PLA–gelatin nano-scaffold and without scaffold. CONCLUSION: We designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold with better performance, which promoted the repair of cartilage injury as a whole, and explained its possible mechanism in accelerating cartilage repair via the PI3K/AKT signaling pathway. |
format | Online Article Text |
id | pubmed-6255055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62550552018-12-11 Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect Yu, Fei Li, Ming Yuan, Zhipeng Rao, Feng Fang, Xingxing Jiang, Baoguo Wen, Yongqiang Zhang, Peixun Int J Nanomedicine Original Research BACKGROUND: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. PURPOSE: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair. METHODS: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone. Postmodel specimens and micro computed tomography (CT) were used to observe any macroscopic morphological changes in the articular cartilage and subchondral bone, whereas multiple staining methods were used to observe all microcosmic morphological changes. Gross scores and Mankin scores were used to evaluate the repair condition. Immunohistochemical staining was employed to detect protein expression. RESULTS: When the repair included the resveratrol–PLA–gelatin porous nano-scaffold, the repaired cartilage and subchondral bone were in better condition. The expression levels of SIRT1, type II collagen, and PI3K/AKT signaling pathway-related proteins (AKT, VEGF, PTEN, Caspase 9, and MMP13) changed significantly. The expression levels of SIRT1,AKT and type II collagen proteins increased significantly, while the expression levels of VEGF, PTEN, Caspase9 and MMP13 proteins decreased significantly compared with the repair included blank porous PLA–gelatin nano-scaffold and without scaffold. CONCLUSION: We designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold with better performance, which promoted the repair of cartilage injury as a whole, and explained its possible mechanism in accelerating cartilage repair via the PI3K/AKT signaling pathway. Dove Medical Press 2018-11-22 /pmc/articles/PMC6255055/ /pubmed/30538463 http://dx.doi.org/10.2147/IJN.S181855 Text en © 2018 Yu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Yu, Fei Li, Ming Yuan, Zhipeng Rao, Feng Fang, Xingxing Jiang, Baoguo Wen, Yongqiang Zhang, Peixun Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
title | Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
title_full | Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
title_fullStr | Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
title_full_unstemmed | Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
title_short | Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
title_sort | mechanism research on a bioactive resveratrol– pla–gelatin porous nano-scaffold in promoting the repair of cartilage defect |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255055/ https://www.ncbi.nlm.nih.gov/pubmed/30538463 http://dx.doi.org/10.2147/IJN.S181855 |
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