Cargando…

Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect

BACKGROUND: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. PURPOSE: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze dry...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Fei, Li, Ming, Yuan, Zhipeng, Rao, Feng, Fang, Xingxing, Jiang, Baoguo, Wen, Yongqiang, Zhang, Peixun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255055/
https://www.ncbi.nlm.nih.gov/pubmed/30538463
http://dx.doi.org/10.2147/IJN.S181855
_version_ 1783373868651511808
author Yu, Fei
Li, Ming
Yuan, Zhipeng
Rao, Feng
Fang, Xingxing
Jiang, Baoguo
Wen, Yongqiang
Zhang, Peixun
author_facet Yu, Fei
Li, Ming
Yuan, Zhipeng
Rao, Feng
Fang, Xingxing
Jiang, Baoguo
Wen, Yongqiang
Zhang, Peixun
author_sort Yu, Fei
collection PubMed
description BACKGROUND: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. PURPOSE: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair. METHODS: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone. Postmodel specimens and micro computed tomography (CT) were used to observe any macroscopic morphological changes in the articular cartilage and subchondral bone, whereas multiple staining methods were used to observe all microcosmic morphological changes. Gross scores and Mankin scores were used to evaluate the repair condition. Immunohistochemical staining was employed to detect protein expression. RESULTS: When the repair included the resveratrol–PLA–gelatin porous nano-scaffold, the repaired cartilage and subchondral bone were in better condition. The expression levels of SIRT1, type II collagen, and PI3K/AKT signaling pathway-related proteins (AKT, VEGF, PTEN, Caspase 9, and MMP13) changed significantly. The expression levels of SIRT1,AKT and type II collagen proteins increased significantly, while the expression levels of VEGF, PTEN, Caspase9 and MMP13 proteins decreased significantly compared with the repair included blank porous PLA–gelatin nano-scaffold and without scaffold. CONCLUSION: We designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold with better performance, which promoted the repair of cartilage injury as a whole, and explained its possible mechanism in accelerating cartilage repair via the PI3K/AKT signaling pathway.
format Online
Article
Text
id pubmed-6255055
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-62550552018-12-11 Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect Yu, Fei Li, Ming Yuan, Zhipeng Rao, Feng Fang, Xingxing Jiang, Baoguo Wen, Yongqiang Zhang, Peixun Int J Nanomedicine Original Research BACKGROUND: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. PURPOSE: In this study, we designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair. METHODS: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone. Postmodel specimens and micro computed tomography (CT) were used to observe any macroscopic morphological changes in the articular cartilage and subchondral bone, whereas multiple staining methods were used to observe all microcosmic morphological changes. Gross scores and Mankin scores were used to evaluate the repair condition. Immunohistochemical staining was employed to detect protein expression. RESULTS: When the repair included the resveratrol–PLA–gelatin porous nano-scaffold, the repaired cartilage and subchondral bone were in better condition. The expression levels of SIRT1, type II collagen, and PI3K/AKT signaling pathway-related proteins (AKT, VEGF, PTEN, Caspase 9, and MMP13) changed significantly. The expression levels of SIRT1,AKT and type II collagen proteins increased significantly, while the expression levels of VEGF, PTEN, Caspase9 and MMP13 proteins decreased significantly compared with the repair included blank porous PLA–gelatin nano-scaffold and without scaffold. CONCLUSION: We designed a bioactive resveratrol–PLA–gelatin porous nano-scaffold with better performance, which promoted the repair of cartilage injury as a whole, and explained its possible mechanism in accelerating cartilage repair via the PI3K/AKT signaling pathway. Dove Medical Press 2018-11-22 /pmc/articles/PMC6255055/ /pubmed/30538463 http://dx.doi.org/10.2147/IJN.S181855 Text en © 2018 Yu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yu, Fei
Li, Ming
Yuan, Zhipeng
Rao, Feng
Fang, Xingxing
Jiang, Baoguo
Wen, Yongqiang
Zhang, Peixun
Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_full Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_fullStr Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_full_unstemmed Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_short Mechanism research on a bioactive resveratrol– PLA–gelatin porous nano-scaffold in promoting the repair of cartilage defect
title_sort mechanism research on a bioactive resveratrol– pla–gelatin porous nano-scaffold in promoting the repair of cartilage defect
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255055/
https://www.ncbi.nlm.nih.gov/pubmed/30538463
http://dx.doi.org/10.2147/IJN.S181855
work_keys_str_mv AT yufei mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT liming mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT yuanzhipeng mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT raofeng mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT fangxingxing mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT jiangbaoguo mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT wenyongqiang mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect
AT zhangpeixun mechanismresearchonabioactiveresveratrolplagelatinporousnanoscaffoldinpromotingtherepairofcartilagedefect