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760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported

BACKGROUND: The seven lineages of Mycobacterium tuberculosis differ in virulence, transmissibility, drug resistance, and immune responses. We reviewed spoligotype data published from India to determine the distribution and diversity of TB lineages in India. METHODS: Spoligoytpe patterns were extract...

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Autores principales: Poonawala, Husain, Verma, Amit, Kumar, Narender
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255662/
http://dx.doi.org/10.1093/ofid/ofy210.767
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author Poonawala, Husain
Verma, Amit
Kumar, Narender
author_facet Poonawala, Husain
Verma, Amit
Kumar, Narender
author_sort Poonawala, Husain
collection PubMed
description BACKGROUND: The seven lineages of Mycobacterium tuberculosis differ in virulence, transmissibility, drug resistance, and immune responses. We reviewed spoligotype data published from India to determine the distribution and diversity of TB lineages in India. METHODS: Spoligoytpe patterns were extracted from studies published from India and compared against the SpolSimilarity database to determine Spoligotype International Type (SIT) number, sub-lineage and lineage. Minimum spanning trees (MSTs) were created with Phyloviz. RESULTS: Thirty-four studies list 1,473 spoligotypes containing 8,242 isolates (Table 1), with 6,733 isolates belonging to 472 SITs. Of the remaining 1,001 patterns (1,509 isolates), 351 patterns (491 isolates) match orphans, while 650 patterns (1,018 isolates) are unreported; these 1,509 isolates could potentially form 532 new SITs and 469 orphans. The most common SITs are SIT11 (Lineage 1; 1,218 isolates, 14.78%), SIT26 (Lineage 3; 982,11.91%) and SIT1 (Lineage 2; 618, 7.5%). Figure 1 demonstrates regional differences in lineage distribution. Figure 2 is an MST of all isolates by lineage (“not assigned” are known SITs with no lineage and “unknowns” are previously unreported patterns). Figure 3 is an MST by superfamily, “others” referring to patterns with no known superfamily. CONCLUSION: Lineages 1 and 3 dominate in India, in contrast to lineages 2 and 4 prevalent worldwide. The 1,473 SITs reflect the significant diversity of M. tuberculosis in India; the 1,001 orphan and previously unreported patterns suggest this diversity is underestimated. Understanding M. tuberculosis diversity is necessary for clinical and public health interventions to control India’s TB epidemic. DISCLOSURES: All authors: No reported disclosures.
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spelling pubmed-62556622018-11-28 760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported Poonawala, Husain Verma, Amit Kumar, Narender Open Forum Infect Dis Abstracts BACKGROUND: The seven lineages of Mycobacterium tuberculosis differ in virulence, transmissibility, drug resistance, and immune responses. We reviewed spoligotype data published from India to determine the distribution and diversity of TB lineages in India. METHODS: Spoligoytpe patterns were extracted from studies published from India and compared against the SpolSimilarity database to determine Spoligotype International Type (SIT) number, sub-lineage and lineage. Minimum spanning trees (MSTs) were created with Phyloviz. RESULTS: Thirty-four studies list 1,473 spoligotypes containing 8,242 isolates (Table 1), with 6,733 isolates belonging to 472 SITs. Of the remaining 1,001 patterns (1,509 isolates), 351 patterns (491 isolates) match orphans, while 650 patterns (1,018 isolates) are unreported; these 1,509 isolates could potentially form 532 new SITs and 469 orphans. The most common SITs are SIT11 (Lineage 1; 1,218 isolates, 14.78%), SIT26 (Lineage 3; 982,11.91%) and SIT1 (Lineage 2; 618, 7.5%). Figure 1 demonstrates regional differences in lineage distribution. Figure 2 is an MST of all isolates by lineage (“not assigned” are known SITs with no lineage and “unknowns” are previously unreported patterns). Figure 3 is an MST by superfamily, “others” referring to patterns with no known superfamily. CONCLUSION: Lineages 1 and 3 dominate in India, in contrast to lineages 2 and 4 prevalent worldwide. The 1,473 SITs reflect the significant diversity of M. tuberculosis in India; the 1,001 orphan and previously unreported patterns suggest this diversity is underestimated. Understanding M. tuberculosis diversity is necessary for clinical and public health interventions to control India’s TB epidemic. DISCLOSURES: All authors: No reported disclosures. Oxford University Press 2018-11-26 /pmc/articles/PMC6255662/ http://dx.doi.org/10.1093/ofid/ofy210.767 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Abstracts
Poonawala, Husain
Verma, Amit
Kumar, Narender
760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported
title 760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported
title_full 760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported
title_fullStr 760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported
title_full_unstemmed 760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported
title_short 760. The Diversity of Mycobacterium tuberculosis in India Is Underestimated and Underreported
title_sort 760. the diversity of mycobacterium tuberculosis in india is underestimated and underreported
topic Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255662/
http://dx.doi.org/10.1093/ofid/ofy210.767
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