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Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats

PURPOSE: Acute normovolemic hemodilution (ANH) is an autologous transfusion method, using blood collected during surgery, to reduce the need for allogeneic blood transfusion. ANH is controversial because it may lead to various complications. Among the possible complications, anastomotic leakage is o...

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Autores principales: Kim, Tae Yeon, Kim, Dong Won, Jeong, Mi Ae, Jun, Jong Hun, Min, Sung Jeong, Shin, Su-Jin, Ha, Tae Kyung, Choi, Dongho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Surgical Society 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255747/
https://www.ncbi.nlm.nih.gov/pubmed/30505822
http://dx.doi.org/10.4174/astr.2018.95.6.312
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author Kim, Tae Yeon
Kim, Dong Won
Jeong, Mi Ae
Jun, Jong Hun
Min, Sung Jeong
Shin, Su-Jin
Ha, Tae Kyung
Choi, Dongho
author_facet Kim, Tae Yeon
Kim, Dong Won
Jeong, Mi Ae
Jun, Jong Hun
Min, Sung Jeong
Shin, Su-Jin
Ha, Tae Kyung
Choi, Dongho
author_sort Kim, Tae Yeon
collection PubMed
description PURPOSE: Acute normovolemic hemodilution (ANH) is an autologous transfusion method, using blood collected during surgery, to reduce the need for allogeneic blood transfusion. ANH is controversial because it may lead to various complications. Among the possible complications, anastomotic leakage is one that would have a significant effect on the operation outcome. However, the relationship between ANH and anastomotic site healing requires additional research. Therefore, we conducted this prospective study of ANH, comparing it with standard intraoperative management, undergoing gastric anastomosis in rats. METHODS: Sixteen Sprague-Dawley rats were randomly assigned to three groups: group A, surgery with ANH; group N, surgery with standard intraoperative management; and group C, sham surgery with standard intraoperative management. ANH was performed in group A animals by, removing 5.8–6.6 mL of blood and replacing it with 3 times as much crystalloid. All rats were enthanized on postoperative day 6, and histopathologic analyses were performed. RESULTS: The mean hematocrit values, after hemodilution were 22.0% (range, 18.0%–29.0%), group A; 33.0% (29.0%–35.0%), group N; and 32.5% (29.0%–34.0%), group C. There were significant differences between groups A and N (P = 0.019, P = 0.009, P = 0.004, P = 0.039, and P = 0.027), and between groups N and C (P = 0.006, P = 0.027, P = 0.04, P = 0.008, and P = 0.009) with respect to inflammatory cell numbers, neovascularization, fibroblast numbers, edema and necrosis, respectively; there were no differences between groups A and N. CONCLUSION: In rat model, anastomotic complications did not increase in the ANH group, compared with the standard intraoperative management group.
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spelling pubmed-62557472018-12-01 Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats Kim, Tae Yeon Kim, Dong Won Jeong, Mi Ae Jun, Jong Hun Min, Sung Jeong Shin, Su-Jin Ha, Tae Kyung Choi, Dongho Ann Surg Treat Res Original Article PURPOSE: Acute normovolemic hemodilution (ANH) is an autologous transfusion method, using blood collected during surgery, to reduce the need for allogeneic blood transfusion. ANH is controversial because it may lead to various complications. Among the possible complications, anastomotic leakage is one that would have a significant effect on the operation outcome. However, the relationship between ANH and anastomotic site healing requires additional research. Therefore, we conducted this prospective study of ANH, comparing it with standard intraoperative management, undergoing gastric anastomosis in rats. METHODS: Sixteen Sprague-Dawley rats were randomly assigned to three groups: group A, surgery with ANH; group N, surgery with standard intraoperative management; and group C, sham surgery with standard intraoperative management. ANH was performed in group A animals by, removing 5.8–6.6 mL of blood and replacing it with 3 times as much crystalloid. All rats were enthanized on postoperative day 6, and histopathologic analyses were performed. RESULTS: The mean hematocrit values, after hemodilution were 22.0% (range, 18.0%–29.0%), group A; 33.0% (29.0%–35.0%), group N; and 32.5% (29.0%–34.0%), group C. There were significant differences between groups A and N (P = 0.019, P = 0.009, P = 0.004, P = 0.039, and P = 0.027), and between groups N and C (P = 0.006, P = 0.027, P = 0.04, P = 0.008, and P = 0.009) with respect to inflammatory cell numbers, neovascularization, fibroblast numbers, edema and necrosis, respectively; there were no differences between groups A and N. CONCLUSION: In rat model, anastomotic complications did not increase in the ANH group, compared with the standard intraoperative management group. The Korean Surgical Society 2018-12 2018-11-26 /pmc/articles/PMC6255747/ /pubmed/30505822 http://dx.doi.org/10.4174/astr.2018.95.6.312 Text en Copyright © 2018, the Korean Surgical Society http://creativecommons.org/licenses/by-nc/4.0/ Annals of Surgical Treatment and Research is an Open Access Journal. All articles are distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Tae Yeon
Kim, Dong Won
Jeong, Mi Ae
Jun, Jong Hun
Min, Sung Jeong
Shin, Su-Jin
Ha, Tae Kyung
Choi, Dongho
Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
title Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
title_full Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
title_fullStr Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
title_full_unstemmed Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
title_short Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
title_sort effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255747/
https://www.ncbi.nlm.nih.gov/pubmed/30505822
http://dx.doi.org/10.4174/astr.2018.95.6.312
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