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Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon
Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255916/ https://www.ncbi.nlm.nih.gov/pubmed/30478396 http://dx.doi.org/10.1038/s41598-018-35790-w |
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author | Kouzai, Yusuke Noutoshi, Yoshiteru Inoue, Komaki Shimizu, Minami Onda, Yoshihiko Mochida, Keiichi |
author_facet | Kouzai, Yusuke Noutoshi, Yoshiteru Inoue, Komaki Shimizu, Minami Onda, Yoshihiko Mochida, Keiichi |
author_sort | Kouzai, Yusuke |
collection | PubMed |
description | Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA, but not BTH, confers resistance against Rhizoctonia solani, the causal agent of sheath blight disease, in Brachypodium distachyon. Here, we demonstrated that BTH compromised the resistance of Bd3-1 and Gaz4, the two sheath blight-resistant accessions of B. distachyon, which activate SA-dependent signaling following challenge by R. solani. Moreover, upon analyzing our published RNA-seq data from B. distachyon treated with SA or BTH, we found that BTH specifically induces expression of genes related to chloroplast function and jasmonic acid (JA) signaling, suggesting that BTH attenuates R. solani resistance by perturbing growth-defense trade-offs and/or by inducing a JA response that may increase susceptibility to R. solani. Our findings demonstrated that BTH does not work as a simple mimic of SA in B. distachyon, and consequently may presumably cause unfavorable side effects through the transcriptional alteration, particularly with respect to R. solani resistance. |
format | Online Article Text |
id | pubmed-6255916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62559162018-12-03 Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon Kouzai, Yusuke Noutoshi, Yoshiteru Inoue, Komaki Shimizu, Minami Onda, Yoshihiko Mochida, Keiichi Sci Rep Article Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA, but not BTH, confers resistance against Rhizoctonia solani, the causal agent of sheath blight disease, in Brachypodium distachyon. Here, we demonstrated that BTH compromised the resistance of Bd3-1 and Gaz4, the two sheath blight-resistant accessions of B. distachyon, which activate SA-dependent signaling following challenge by R. solani. Moreover, upon analyzing our published RNA-seq data from B. distachyon treated with SA or BTH, we found that BTH specifically induces expression of genes related to chloroplast function and jasmonic acid (JA) signaling, suggesting that BTH attenuates R. solani resistance by perturbing growth-defense trade-offs and/or by inducing a JA response that may increase susceptibility to R. solani. Our findings demonstrated that BTH does not work as a simple mimic of SA in B. distachyon, and consequently may presumably cause unfavorable side effects through the transcriptional alteration, particularly with respect to R. solani resistance. Nature Publishing Group UK 2018-11-26 /pmc/articles/PMC6255916/ /pubmed/30478396 http://dx.doi.org/10.1038/s41598-018-35790-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kouzai, Yusuke Noutoshi, Yoshiteru Inoue, Komaki Shimizu, Minami Onda, Yoshihiko Mochida, Keiichi Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon |
title | Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon |
title_full | Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon |
title_fullStr | Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon |
title_full_unstemmed | Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon |
title_short | Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon |
title_sort | benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in brachypodium distachyon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255916/ https://www.ncbi.nlm.nih.gov/pubmed/30478396 http://dx.doi.org/10.1038/s41598-018-35790-w |
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