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MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that m...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256845/ https://www.ncbi.nlm.nih.gov/pubmed/30546411 http://dx.doi.org/10.3892/etm.2018.6888 |
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author | Cao, Wei Fang, Le Teng, Siyong Chen, Hongwei Liu, Tiejun |
author_facet | Cao, Wei Fang, Le Teng, Siyong Chen, Hongwei Liu, Tiejun |
author_sort | Cao, Wei |
collection | PubMed |
description | Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that miR-466 was significantly downregulated in OS tissues and cell lines. Furthermore, it was revealed that the expression of miR-466 was negatively correlated with OS severity. Moreover, low miR-466 expression in patients with OS predicted poor prognosis. Through functional experiments, miR-466 overexpression significantly inhibited the proliferation and cell cycle of OS cells while inducing cellular apoptosis. In terms of mechanism, it was revealed that CCND1 was a target of miR-466 in OS cells. miR-466 overexpression suppressed CCND1 expression in OS cells. A reverse association was observed between the expression levels of miR-466 and CCND1 in OS tissues. Furthermore, CCND1 restoration in OS cells significantly rescued the effects of miR-466 on cellular proliferation and apoptosis. Overall, the results of the present study demonstrated that miR-466 suppressed OS progression by targeting CCND1, suggesting that miR-466 may be a promising biomarker and therapeutic target for OS prognosis and treatment. |
format | Online Article Text |
id | pubmed-6256845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-62568452018-12-13 MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 Cao, Wei Fang, Le Teng, Siyong Chen, Hongwei Liu, Tiejun Exp Ther Med Articles Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that miR-466 was significantly downregulated in OS tissues and cell lines. Furthermore, it was revealed that the expression of miR-466 was negatively correlated with OS severity. Moreover, low miR-466 expression in patients with OS predicted poor prognosis. Through functional experiments, miR-466 overexpression significantly inhibited the proliferation and cell cycle of OS cells while inducing cellular apoptosis. In terms of mechanism, it was revealed that CCND1 was a target of miR-466 in OS cells. miR-466 overexpression suppressed CCND1 expression in OS cells. A reverse association was observed between the expression levels of miR-466 and CCND1 in OS tissues. Furthermore, CCND1 restoration in OS cells significantly rescued the effects of miR-466 on cellular proliferation and apoptosis. Overall, the results of the present study demonstrated that miR-466 suppressed OS progression by targeting CCND1, suggesting that miR-466 may be a promising biomarker and therapeutic target for OS prognosis and treatment. D.A. Spandidos 2018-12 2018-10-22 /pmc/articles/PMC6256845/ /pubmed/30546411 http://dx.doi.org/10.3892/etm.2018.6888 Text en Copyright: © Cao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Cao, Wei Fang, Le Teng, Siyong Chen, Hongwei Liu, Tiejun MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 |
title | MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 |
title_full | MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 |
title_fullStr | MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 |
title_full_unstemmed | MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 |
title_short | MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 |
title_sort | microrna-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting ccnd1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256845/ https://www.ncbi.nlm.nih.gov/pubmed/30546411 http://dx.doi.org/10.3892/etm.2018.6888 |
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