Cargando…

MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1

Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that m...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Wei, Fang, Le, Teng, Siyong, Chen, Hongwei, Liu, Tiejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256845/
https://www.ncbi.nlm.nih.gov/pubmed/30546411
http://dx.doi.org/10.3892/etm.2018.6888
_version_ 1783374225223974912
author Cao, Wei
Fang, Le
Teng, Siyong
Chen, Hongwei
Liu, Tiejun
author_facet Cao, Wei
Fang, Le
Teng, Siyong
Chen, Hongwei
Liu, Tiejun
author_sort Cao, Wei
collection PubMed
description Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that miR-466 was significantly downregulated in OS tissues and cell lines. Furthermore, it was revealed that the expression of miR-466 was negatively correlated with OS severity. Moreover, low miR-466 expression in patients with OS predicted poor prognosis. Through functional experiments, miR-466 overexpression significantly inhibited the proliferation and cell cycle of OS cells while inducing cellular apoptosis. In terms of mechanism, it was revealed that CCND1 was a target of miR-466 in OS cells. miR-466 overexpression suppressed CCND1 expression in OS cells. A reverse association was observed between the expression levels of miR-466 and CCND1 in OS tissues. Furthermore, CCND1 restoration in OS cells significantly rescued the effects of miR-466 on cellular proliferation and apoptosis. Overall, the results of the present study demonstrated that miR-466 suppressed OS progression by targeting CCND1, suggesting that miR-466 may be a promising biomarker and therapeutic target for OS prognosis and treatment.
format Online
Article
Text
id pubmed-6256845
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-62568452018-12-13 MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1 Cao, Wei Fang, Le Teng, Siyong Chen, Hongwei Liu, Tiejun Exp Ther Med Articles Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that miR-466 was significantly downregulated in OS tissues and cell lines. Furthermore, it was revealed that the expression of miR-466 was negatively correlated with OS severity. Moreover, low miR-466 expression in patients with OS predicted poor prognosis. Through functional experiments, miR-466 overexpression significantly inhibited the proliferation and cell cycle of OS cells while inducing cellular apoptosis. In terms of mechanism, it was revealed that CCND1 was a target of miR-466 in OS cells. miR-466 overexpression suppressed CCND1 expression in OS cells. A reverse association was observed between the expression levels of miR-466 and CCND1 in OS tissues. Furthermore, CCND1 restoration in OS cells significantly rescued the effects of miR-466 on cellular proliferation and apoptosis. Overall, the results of the present study demonstrated that miR-466 suppressed OS progression by targeting CCND1, suggesting that miR-466 may be a promising biomarker and therapeutic target for OS prognosis and treatment. D.A. Spandidos 2018-12 2018-10-22 /pmc/articles/PMC6256845/ /pubmed/30546411 http://dx.doi.org/10.3892/etm.2018.6888 Text en Copyright: © Cao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Cao, Wei
Fang, Le
Teng, Siyong
Chen, Hongwei
Liu, Tiejun
MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
title MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
title_full MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
title_fullStr MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
title_full_unstemmed MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
title_short MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1
title_sort microrna-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting ccnd1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256845/
https://www.ncbi.nlm.nih.gov/pubmed/30546411
http://dx.doi.org/10.3892/etm.2018.6888
work_keys_str_mv AT caowei microrna466inhibitsosteosarcomacellproliferationandinducesapoptosisbytargetingccnd1
AT fangle microrna466inhibitsosteosarcomacellproliferationandinducesapoptosisbytargetingccnd1
AT tengsiyong microrna466inhibitsosteosarcomacellproliferationandinducesapoptosisbytargetingccnd1
AT chenhongwei microrna466inhibitsosteosarcomacellproliferationandinducesapoptosisbytargetingccnd1
AT liutiejun microrna466inhibitsosteosarcomacellproliferationandinducesapoptosisbytargetingccnd1