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Detection of HIV cDNA Point Mutations with Rolling-Circle Amplification Arrays

In this paper we describe an isothermal rolling-circle amplification (RCA) protocol to detect gene point mutations on chips. The method is based on an allele-specific oligonucleotide circularization mediated by a special DNA ligase. The probe is circularized when perfect complementary sequences betw...

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Detalles Bibliográficos
Autores principales:	Wu, Lingwei, Liu, Quanjun, Wu, Zhongwei, Lu, Zuhong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Molecular Diversity Preservation International 2010
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256933/ https://www.ncbi.nlm.nih.gov/pubmed/20335932 http://dx.doi.org/10.3390/molecules15020619

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