Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE

Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic dete...

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Detalles Bibliográficos
Autores principales: Giljanović, Josipa, Prkić, Ante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257127/
https://www.ncbi.nlm.nih.gov/pubmed/20110875
http://dx.doi.org/10.3390/molecules15010100
Descripción
Sumario:Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide CoASH concentration range (1×10(-6) – 1×10(-4) M).

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