Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE

Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic dete...

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Detalles Bibliográficos
Autores principales: Giljanović, Josipa, Prkić, Ante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257127/
https://www.ncbi.nlm.nih.gov/pubmed/20110875
http://dx.doi.org/10.3390/molecules15010100
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author Giljanović, Josipa
Prkić, Ante
author_facet Giljanović, Josipa
Prkić, Ante
author_sort Giljanović, Josipa
collection PubMed
description Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide CoASH concentration range (1×10(-6) – 1×10(-4) M).
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spelling pubmed-62571272018-12-03 Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE Giljanović, Josipa Prkić, Ante Molecules Article Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide CoASH concentration range (1×10(-6) – 1×10(-4) M). Molecular Diversity Preservation International 2009-12-29 /pmc/articles/PMC6257127/ /pubmed/20110875 http://dx.doi.org/10.3390/molecules15010100 Text en © 2010 by the authors; http://creativecommons.org/licenses/by/3.0/ licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Giljanović, Josipa
Prkić, Ante
Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
title Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
title_full Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
title_fullStr Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
title_full_unstemmed Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
title_short Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
title_sort determination of coenzyme a (coash) in the presence of different thiols by using flow-injection with a uv/vis spectrophotometric detector and potentiometric determination of coash using an iodide ise
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257127/
https://www.ncbi.nlm.nih.gov/pubmed/20110875
http://dx.doi.org/10.3390/molecules15010100
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