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Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway

PURPOSE: The purpose of this study was to assess the effects of biomimetic intrafibrillar mineralized collagen (IMC) bone scaffold materials on bone regeneration and the underlying biological mechanisms. MATERIALS AND METHODS: A critical-sized bone defect in the rat femur was created; then IMC, extr...

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Autores principales: Zhang, Zhen, Li, Zheyi, Zhang, Chengyao, Liu, Jiannan, Bai, Yuxing, Li, Song, Zhang, Chenping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257138/
https://www.ncbi.nlm.nih.gov/pubmed/30538446
http://dx.doi.org/10.2147/IJN.S172164
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author Zhang, Zhen
Li, Zheyi
Zhang, Chengyao
Liu, Jiannan
Bai, Yuxing
Li, Song
Zhang, Chenping
author_facet Zhang, Zhen
Li, Zheyi
Zhang, Chengyao
Liu, Jiannan
Bai, Yuxing
Li, Song
Zhang, Chenping
author_sort Zhang, Zhen
collection PubMed
description PURPOSE: The purpose of this study was to assess the effects of biomimetic intrafibrillar mineralized collagen (IMC) bone scaffold materials on bone regeneration and the underlying biological mechanisms. MATERIALS AND METHODS: A critical-sized bone defect in the rat femur was created; then IMC, extrafibrillar mineralized collagen, and nano-hydroxyapatite bone scaffold materials were grafted into the defect. Ten weeks after implantation, micro-computed tomography and histology were applied to evaluate the bone regeneration. Furthermore, microarray technology was applied for transcriptional profile analysis at two postoperative time points (7 and 14 days). Subsequently, the critical genes involved in bone regeneration identified by transcriptional analysis were verified both in vivo through immunohistochemical analysis and in vitro by quantitative real-time transcription polymerase chain reaction evaluation. RESULTS: Significantly increased new bone formation was found in the IMC group based on micro-computed tomography and histological evaluation (P<0.05). Transcriptional analysis revealed that the early process of IMC-guided bone regeneration involves the overexpression of genes mainly associated with inflammation, immune response, skeletal development, angiogenesis, neurogenesis, and the Wnt signaling pathway. The roles of the Wnt signaling pathway-related factors Wnt5a, β-catenin, and Axin2 were further confirmed both in vivo and in vitro. CONCLUSION: The IMC bone scaffold materials significantly enhanced bone regeneration via activation of the Wnt signaling pathway.
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spelling pubmed-62571382018-12-11 Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway Zhang, Zhen Li, Zheyi Zhang, Chengyao Liu, Jiannan Bai, Yuxing Li, Song Zhang, Chenping Int J Nanomedicine Original Research PURPOSE: The purpose of this study was to assess the effects of biomimetic intrafibrillar mineralized collagen (IMC) bone scaffold materials on bone regeneration and the underlying biological mechanisms. MATERIALS AND METHODS: A critical-sized bone defect in the rat femur was created; then IMC, extrafibrillar mineralized collagen, and nano-hydroxyapatite bone scaffold materials were grafted into the defect. Ten weeks after implantation, micro-computed tomography and histology were applied to evaluate the bone regeneration. Furthermore, microarray technology was applied for transcriptional profile analysis at two postoperative time points (7 and 14 days). Subsequently, the critical genes involved in bone regeneration identified by transcriptional analysis were verified both in vivo through immunohistochemical analysis and in vitro by quantitative real-time transcription polymerase chain reaction evaluation. RESULTS: Significantly increased new bone formation was found in the IMC group based on micro-computed tomography and histological evaluation (P<0.05). Transcriptional analysis revealed that the early process of IMC-guided bone regeneration involves the overexpression of genes mainly associated with inflammation, immune response, skeletal development, angiogenesis, neurogenesis, and the Wnt signaling pathway. The roles of the Wnt signaling pathway-related factors Wnt5a, β-catenin, and Axin2 were further confirmed both in vivo and in vitro. CONCLUSION: The IMC bone scaffold materials significantly enhanced bone regeneration via activation of the Wnt signaling pathway. Dove Medical Press 2018-11-21 /pmc/articles/PMC6257138/ /pubmed/30538446 http://dx.doi.org/10.2147/IJN.S172164 Text en © 2018 Zhang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhang, Zhen
Li, Zheyi
Zhang, Chengyao
Liu, Jiannan
Bai, Yuxing
Li, Song
Zhang, Chenping
Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway
title Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway
title_full Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway
title_fullStr Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway
title_full_unstemmed Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway
title_short Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway
title_sort biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the wnt signaling pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257138/
https://www.ncbi.nlm.nih.gov/pubmed/30538446
http://dx.doi.org/10.2147/IJN.S172164
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