The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells

Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-medi...

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Autores principales: Bronikowska, Joanna, Szliszka, Ewelina, Czuba, Zenon P., Zwolinski, Dariusz, Szmydki, Dariusz, Krol, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257263/
https://www.ncbi.nlm.nih.gov/pubmed/20336028
http://dx.doi.org/10.3390/molecules15032000
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author Bronikowska, Joanna
Szliszka, Ewelina
Czuba, Zenon P.
Zwolinski, Dariusz
Szmydki, Dariusz
Krol, Wojciech
author_facet Bronikowska, Joanna
Szliszka, Ewelina
Czuba, Zenon P.
Zwolinski, Dariusz
Szmydki, Dariusz
Krol, Wojciech
author_sort Bronikowska, Joanna
collection PubMed
description Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-mediated apoptosis in cancer cells. We examined the cytotoxic and apoptotic activities of isoflavones in combination with TRAIL on HeLa cancer cells. The apoptosis was detected by fluorescence microscopy with annexin V-FITC. The cytotoxicity was evaluated by MTT and LDH assays. The tested isoflavones: genistein, biochanin-A and neobavaisoflavone enhance TRAIL-induced apoptosis in HeLa cells. Our study indicated that isoflavones augmented TRAIL-cytotoxicity against cancer cells and confirmed potential role of those polyphenols in chemoprevention.
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spelling pubmed-62572632018-12-04 The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells Bronikowska, Joanna Szliszka, Ewelina Czuba, Zenon P. Zwolinski, Dariusz Szmydki, Dariusz Krol, Wojciech Molecules Article Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-mediated apoptosis in cancer cells. We examined the cytotoxic and apoptotic activities of isoflavones in combination with TRAIL on HeLa cancer cells. The apoptosis was detected by fluorescence microscopy with annexin V-FITC. The cytotoxicity was evaluated by MTT and LDH assays. The tested isoflavones: genistein, biochanin-A and neobavaisoflavone enhance TRAIL-induced apoptosis in HeLa cells. Our study indicated that isoflavones augmented TRAIL-cytotoxicity against cancer cells and confirmed potential role of those polyphenols in chemoprevention. Molecular Diversity Preservation International 2010-03-22 /pmc/articles/PMC6257263/ /pubmed/20336028 http://dx.doi.org/10.3390/molecules15032000 Text en © 2010 by the authors; http://creativecommons.org/licenses/by/3.0/ licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Bronikowska, Joanna
Szliszka, Ewelina
Czuba, Zenon P.
Zwolinski, Dariusz
Szmydki, Dariusz
Krol, Wojciech
The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
title The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
title_full The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
title_fullStr The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
title_full_unstemmed The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
title_short The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
title_sort combination of trail and isoflavones enhances apoptosis in cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257263/
https://www.ncbi.nlm.nih.gov/pubmed/20336028
http://dx.doi.org/10.3390/molecules15032000
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