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Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
The effect of epimedium extracting solution on bone mineral density (BMD) of steroid-induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague-Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257266/ https://www.ncbi.nlm.nih.gov/pubmed/30542458 http://dx.doi.org/10.3892/etm.2018.6827 |
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author | Liu, Su Huang, Yunzong Wang, Chuangli Tian, Shoujing Xu, Youjia Ge, Jianfei |
author_facet | Liu, Su Huang, Yunzong Wang, Chuangli Tian, Shoujing Xu, Youjia Ge, Jianfei |
author_sort | Liu, Su |
collection | PubMed |
description | The effect of epimedium extracting solution on bone mineral density (BMD) of steroid-induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague-Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: the control (n=8), the glucocorticoid (n=8) and the epimedium (n=8) group. Rats in the glucocorticoid and the epimedium group were injected with prednisolone acetate injection in gluteal muscles with 15 mg/kg/day twice a week. The epimedium group was given 10 ml/kg ephedra extracting solution containing crude drug with the concentration of 1.5 g/ml daily by gavage. After 6 weeks, all the experimental rats were sacrificed and materials were extracted. The expression of autophagy-related proteins were detected by observing the bone of the femoral head. After comparison of the control group with the model group in BMD, it was found that there were significant differences (P<0.05). There were no significant differences between the control and the epimedium group (P>0.05). Neither between the glucocorticoid and the epimedium group (P<0.05). Epimedium extracting solution can significantly enhance the BMD of femoral heads, prevent osteoporosis and lead to collapse, increase the expression of apoptotic and protective proteins and reduce the expression of autophagy-related proteins, thus providing a preliminary theoretical study for the prevention and treatment of SANFH. |
format | Online Article Text |
id | pubmed-6257266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-62572662018-12-12 Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy Liu, Su Huang, Yunzong Wang, Chuangli Tian, Shoujing Xu, Youjia Ge, Jianfei Exp Ther Med Articles The effect of epimedium extracting solution on bone mineral density (BMD) of steroid-induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague-Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: the control (n=8), the glucocorticoid (n=8) and the epimedium (n=8) group. Rats in the glucocorticoid and the epimedium group were injected with prednisolone acetate injection in gluteal muscles with 15 mg/kg/day twice a week. The epimedium group was given 10 ml/kg ephedra extracting solution containing crude drug with the concentration of 1.5 g/ml daily by gavage. After 6 weeks, all the experimental rats were sacrificed and materials were extracted. The expression of autophagy-related proteins were detected by observing the bone of the femoral head. After comparison of the control group with the model group in BMD, it was found that there were significant differences (P<0.05). There were no significant differences between the control and the epimedium group (P>0.05). Neither between the glucocorticoid and the epimedium group (P<0.05). Epimedium extracting solution can significantly enhance the BMD of femoral heads, prevent osteoporosis and lead to collapse, increase the expression of apoptotic and protective proteins and reduce the expression of autophagy-related proteins, thus providing a preliminary theoretical study for the prevention and treatment of SANFH. D.A. Spandidos 2018-12 2018-10-03 /pmc/articles/PMC6257266/ /pubmed/30542458 http://dx.doi.org/10.3892/etm.2018.6827 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Su Huang, Yunzong Wang, Chuangli Tian, Shoujing Xu, Youjia Ge, Jianfei Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
title | Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
title_full | Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
title_fullStr | Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
title_full_unstemmed | Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
title_short | Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
title_sort | epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257266/ https://www.ncbi.nlm.nih.gov/pubmed/30542458 http://dx.doi.org/10.3892/etm.2018.6827 |
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