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Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy

The effect of epimedium extracting solution on bone mineral density (BMD) of steroid-induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague-Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: th...

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Autores principales: Liu, Su, Huang, Yunzong, Wang, Chuangli, Tian, Shoujing, Xu, Youjia, Ge, Jianfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257266/
https://www.ncbi.nlm.nih.gov/pubmed/30542458
http://dx.doi.org/10.3892/etm.2018.6827
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author Liu, Su
Huang, Yunzong
Wang, Chuangli
Tian, Shoujing
Xu, Youjia
Ge, Jianfei
author_facet Liu, Su
Huang, Yunzong
Wang, Chuangli
Tian, Shoujing
Xu, Youjia
Ge, Jianfei
author_sort Liu, Su
collection PubMed
description The effect of epimedium extracting solution on bone mineral density (BMD) of steroid-induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague-Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: the control (n=8), the glucocorticoid (n=8) and the epimedium (n=8) group. Rats in the glucocorticoid and the epimedium group were injected with prednisolone acetate injection in gluteal muscles with 15 mg/kg/day twice a week. The epimedium group was given 10 ml/kg ephedra extracting solution containing crude drug with the concentration of 1.5 g/ml daily by gavage. After 6 weeks, all the experimental rats were sacrificed and materials were extracted. The expression of autophagy-related proteins were detected by observing the bone of the femoral head. After comparison of the control group with the model group in BMD, it was found that there were significant differences (P<0.05). There were no significant differences between the control and the epimedium group (P>0.05). Neither between the glucocorticoid and the epimedium group (P<0.05). Epimedium extracting solution can significantly enhance the BMD of femoral heads, prevent osteoporosis and lead to collapse, increase the expression of apoptotic and protective proteins and reduce the expression of autophagy-related proteins, thus providing a preliminary theoretical study for the prevention and treatment of SANFH.
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spelling pubmed-62572662018-12-12 Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy Liu, Su Huang, Yunzong Wang, Chuangli Tian, Shoujing Xu, Youjia Ge, Jianfei Exp Ther Med Articles The effect of epimedium extracting solution on bone mineral density (BMD) of steroid-induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague-Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: the control (n=8), the glucocorticoid (n=8) and the epimedium (n=8) group. Rats in the glucocorticoid and the epimedium group were injected with prednisolone acetate injection in gluteal muscles with 15 mg/kg/day twice a week. The epimedium group was given 10 ml/kg ephedra extracting solution containing crude drug with the concentration of 1.5 g/ml daily by gavage. After 6 weeks, all the experimental rats were sacrificed and materials were extracted. The expression of autophagy-related proteins were detected by observing the bone of the femoral head. After comparison of the control group with the model group in BMD, it was found that there were significant differences (P<0.05). There were no significant differences between the control and the epimedium group (P>0.05). Neither between the glucocorticoid and the epimedium group (P<0.05). Epimedium extracting solution can significantly enhance the BMD of femoral heads, prevent osteoporosis and lead to collapse, increase the expression of apoptotic and protective proteins and reduce the expression of autophagy-related proteins, thus providing a preliminary theoretical study for the prevention and treatment of SANFH. D.A. Spandidos 2018-12 2018-10-03 /pmc/articles/PMC6257266/ /pubmed/30542458 http://dx.doi.org/10.3892/etm.2018.6827 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Su
Huang, Yunzong
Wang, Chuangli
Tian, Shoujing
Xu, Youjia
Ge, Jianfei
Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
title Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
title_full Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
title_fullStr Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
title_full_unstemmed Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
title_short Epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
title_sort epimedium protects steroid-induced avascular necrosis of femoral head in rats by inhibiting autophagy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257266/
https://www.ncbi.nlm.nih.gov/pubmed/30542458
http://dx.doi.org/10.3892/etm.2018.6827
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