Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus

In this study we investigated the antimicrobial activity of magnolol on Staphylococcus aureus. The minimal inhibitory concentrations of magnolol against 31 S. aureus strains ranged from 4–32 μg/mL. In addition, hemolysin assays, Western blotting, and real-time RT-PCR were performed to investigate th...

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Detalles Bibliográficos
Autores principales: Xiang, Hua, Qiu, Jia-Zhang, Wang, Da-Cheng, Jiang, You-Shuai, Xia, Li-Jie, Deng, Xu-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257330/
https://www.ncbi.nlm.nih.gov/pubmed/20336007
http://dx.doi.org/10.3390/molecules15031679
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author Xiang, Hua
Qiu, Jia-Zhang
Wang, Da-Cheng
Jiang, You-Shuai
Xia, Li-Jie
Deng, Xu-Ming
author_facet Xiang, Hua
Qiu, Jia-Zhang
Wang, Da-Cheng
Jiang, You-Shuai
Xia, Li-Jie
Deng, Xu-Ming
author_sort Xiang, Hua
collection PubMed
description In this study we investigated the antimicrobial activity of magnolol on Staphylococcus aureus. The minimal inhibitory concentrations of magnolol against 31 S. aureus strains ranged from 4–32 μg/mL. In addition, hemolysin assays, Western blotting, and real-time RT-PCR were performed to investigate the effect of magnolol on α-toxin secretion by both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). The results indicated that sub-inhibitory concentrations of magnolol dose-dependently inhibited the transcription of hla (the gene encoding α-toxin) in S. aureus, resulting in a reduction of α-toxin secretion and, thus, hemolytic activities.
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spelling pubmed-62573302018-12-04 Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus Xiang, Hua Qiu, Jia-Zhang Wang, Da-Cheng Jiang, You-Shuai Xia, Li-Jie Deng, Xu-Ming Molecules Article In this study we investigated the antimicrobial activity of magnolol on Staphylococcus aureus. The minimal inhibitory concentrations of magnolol against 31 S. aureus strains ranged from 4–32 μg/mL. In addition, hemolysin assays, Western blotting, and real-time RT-PCR were performed to investigate the effect of magnolol on α-toxin secretion by both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). The results indicated that sub-inhibitory concentrations of magnolol dose-dependently inhibited the transcription of hla (the gene encoding α-toxin) in S. aureus, resulting in a reduction of α-toxin secretion and, thus, hemolytic activities. Molecular Diversity Preservation International 2010-03-12 /pmc/articles/PMC6257330/ /pubmed/20336007 http://dx.doi.org/10.3390/molecules15031679 Text en © 2010 by the authors; http://creativecommons.org/licenses/by/3.0/ licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Xiang, Hua
Qiu, Jia-Zhang
Wang, Da-Cheng
Jiang, You-Shuai
Xia, Li-Jie
Deng, Xu-Ming
Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus
title Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus
title_full Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus
title_fullStr Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus
title_full_unstemmed Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus
title_short Influence of Magnolol on the Secretion of α-Toxin by Staphylococcus aureus
title_sort influence of magnolol on the secretion of α-toxin by staphylococcus aureus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257330/
https://www.ncbi.nlm.nih.gov/pubmed/20336007
http://dx.doi.org/10.3390/molecules15031679
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