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Experimental research on ADSCs-NCSS in wound repair

New collagen sponge scaffold (NCSS) combined with adipose-derived stem cells (ADSCs) in the repair of full-thickness skin wound in nude mice was investigated. Human ADSCs were extracted via enzyme digestion; NCSS materials were prepared using modified method; the tissue-engineered skin substitute wa...

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Autores principales: Zhang, Ai-Jun, Jiang, Tao, Li, Qiang, Jin, Pei-Sheng, Tan, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257557/
https://www.ncbi.nlm.nih.gov/pubmed/30542393
http://dx.doi.org/10.3892/etm.2018.6756
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author Zhang, Ai-Jun
Jiang, Tao
Li, Qiang
Jin, Pei-Sheng
Tan, Qian
author_facet Zhang, Ai-Jun
Jiang, Tao
Li, Qiang
Jin, Pei-Sheng
Tan, Qian
author_sort Zhang, Ai-Jun
collection PubMed
description New collagen sponge scaffold (NCSS) combined with adipose-derived stem cells (ADSCs) in the repair of full-thickness skin wound in nude mice was investigated. Human ADSCs were extracted via enzyme digestion; NCSS materials were prepared using modified method; the tissue-engineered skin substitute was constructed using ADSCs combined with NCSS. Two 10 mm(2) full-thickness skin wounds were designed on the back of 24 female nude mice, respectively. Mice were divided into 4 groups in the experiment: ADSCs-NCSS (group A), simple NCSS (group B), simple ADSCs (group C) and blank control (group D). The wound healing rates were observed at 3, 7, 10 and 14 days after operation, and specimens were taken at 1 and 2 weeks for histological detection and immunohistochemical cluster of differentiation 31 (CD31) vascular density detection, respectively. At 3 and 7 days after construction of new tissue-engineered skin substitute, the infiltration of ADSCs could be seen within NCSS. The wound healing rates at 7, 10 and 14 days after operation in group A were (77.13±1.25%), (89.90±1.08%) and (96.08±0.6%), respectively, which were significantly higher than those in groups B-D; the differences were statistically significant (p<0.05). The detection of regenerated wound tissue thickness at 1 and 2 weeks after operation and CD31 vascular density at 1 week after operation showed that the vascular density in the wound in group A was significantly higher than those in other groups; the differences were statistically significant (p<0.05). After the transplantation of tissue-engineered skin constructed by human ADSCs combined with NCSS, the quality of wound healing in nude mice can be significantly improved, and the wound repair can be promoted.
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spelling pubmed-62575572018-12-12 Experimental research on ADSCs-NCSS in wound repair Zhang, Ai-Jun Jiang, Tao Li, Qiang Jin, Pei-Sheng Tan, Qian Exp Ther Med Articles New collagen sponge scaffold (NCSS) combined with adipose-derived stem cells (ADSCs) in the repair of full-thickness skin wound in nude mice was investigated. Human ADSCs were extracted via enzyme digestion; NCSS materials were prepared using modified method; the tissue-engineered skin substitute was constructed using ADSCs combined with NCSS. Two 10 mm(2) full-thickness skin wounds were designed on the back of 24 female nude mice, respectively. Mice were divided into 4 groups in the experiment: ADSCs-NCSS (group A), simple NCSS (group B), simple ADSCs (group C) and blank control (group D). The wound healing rates were observed at 3, 7, 10 and 14 days after operation, and specimens were taken at 1 and 2 weeks for histological detection and immunohistochemical cluster of differentiation 31 (CD31) vascular density detection, respectively. At 3 and 7 days after construction of new tissue-engineered skin substitute, the infiltration of ADSCs could be seen within NCSS. The wound healing rates at 7, 10 and 14 days after operation in group A were (77.13±1.25%), (89.90±1.08%) and (96.08±0.6%), respectively, which were significantly higher than those in groups B-D; the differences were statistically significant (p<0.05). The detection of regenerated wound tissue thickness at 1 and 2 weeks after operation and CD31 vascular density at 1 week after operation showed that the vascular density in the wound in group A was significantly higher than those in other groups; the differences were statistically significant (p<0.05). After the transplantation of tissue-engineered skin constructed by human ADSCs combined with NCSS, the quality of wound healing in nude mice can be significantly improved, and the wound repair can be promoted. D.A. Spandidos 2018-12 2018-09-18 /pmc/articles/PMC6257557/ /pubmed/30542393 http://dx.doi.org/10.3892/etm.2018.6756 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Ai-Jun
Jiang, Tao
Li, Qiang
Jin, Pei-Sheng
Tan, Qian
Experimental research on ADSCs-NCSS in wound repair
title Experimental research on ADSCs-NCSS in wound repair
title_full Experimental research on ADSCs-NCSS in wound repair
title_fullStr Experimental research on ADSCs-NCSS in wound repair
title_full_unstemmed Experimental research on ADSCs-NCSS in wound repair
title_short Experimental research on ADSCs-NCSS in wound repair
title_sort experimental research on adscs-ncss in wound repair
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257557/
https://www.ncbi.nlm.nih.gov/pubmed/30542393
http://dx.doi.org/10.3892/etm.2018.6756
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