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miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells
The mortality rate of non-small cell lung cancer (NSCLC) remains high worldwide. miR-21-5p plays an important part in many cancer types, including NSCLC. However, the effect of miR-21-5p in NSCLC tumorigenesis remains poorly understood. The present study investigated whether miR-21-5p promoted NSCLC...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257667/ https://www.ncbi.nlm.nih.gov/pubmed/30542417 http://dx.doi.org/10.3892/etm.2018.6752 |
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author | Yan, Liang Ma, Jinzhu Wang, Yi Zan, Jiawei Wang, Zhen Zhu, Yu Zhu, Yiping Ling, Liefeng Cao, Long Liu, Xin Li, Shu Xu, Lei Qi, Zhilin Nie, Liuwang Zhang, Yao |
author_facet | Yan, Liang Ma, Jinzhu Wang, Yi Zan, Jiawei Wang, Zhen Zhu, Yu Zhu, Yiping Ling, Liefeng Cao, Long Liu, Xin Li, Shu Xu, Lei Qi, Zhilin Nie, Liuwang Zhang, Yao |
author_sort | Yan, Liang |
collection | PubMed |
description | The mortality rate of non-small cell lung cancer (NSCLC) remains high worldwide. miR-21-5p plays an important part in many cancer types, including NSCLC. However, the effect of miR-21-5p in NSCLC tumorigenesis remains poorly understood. The present study investigated whether miR-21-5p promoted NSCLC cell proliferation in vitro. In order to study the molecular mechanism by which miR-21-5p contributes to NSCLC progression, three bioinformatics algorithms were used to predict the genes which miR-21-5p targeted. TGFBI was identfieid as a putative direct target in NSCLC cells via the luciferase reporter assay. Furthermore, miR-21-5p downregulated TGFBI protein expression by a post-transcriptional mechanism via western blotting and a reverse transcription-quantitative polymerase chain reaction analysis. Finally, TGFBI exhibited opposing effects to those of miR-21-5p on NSCLC cells, suggesting that miR-21-5p may promote cell proliferation by negative regulation of TGFBI. These results suggest miR-21-5p promote the proliferation of NSCLC cells via inhibiting TGFBI expression. |
format | Online Article Text |
id | pubmed-6257667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-62576672018-12-12 miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells Yan, Liang Ma, Jinzhu Wang, Yi Zan, Jiawei Wang, Zhen Zhu, Yu Zhu, Yiping Ling, Liefeng Cao, Long Liu, Xin Li, Shu Xu, Lei Qi, Zhilin Nie, Liuwang Zhang, Yao Exp Ther Med Articles The mortality rate of non-small cell lung cancer (NSCLC) remains high worldwide. miR-21-5p plays an important part in many cancer types, including NSCLC. However, the effect of miR-21-5p in NSCLC tumorigenesis remains poorly understood. The present study investigated whether miR-21-5p promoted NSCLC cell proliferation in vitro. In order to study the molecular mechanism by which miR-21-5p contributes to NSCLC progression, three bioinformatics algorithms were used to predict the genes which miR-21-5p targeted. TGFBI was identfieid as a putative direct target in NSCLC cells via the luciferase reporter assay. Furthermore, miR-21-5p downregulated TGFBI protein expression by a post-transcriptional mechanism via western blotting and a reverse transcription-quantitative polymerase chain reaction analysis. Finally, TGFBI exhibited opposing effects to those of miR-21-5p on NSCLC cells, suggesting that miR-21-5p may promote cell proliferation by negative regulation of TGFBI. These results suggest miR-21-5p promote the proliferation of NSCLC cells via inhibiting TGFBI expression. D.A. Spandidos 2018-12 2018-09-18 /pmc/articles/PMC6257667/ /pubmed/30542417 http://dx.doi.org/10.3892/etm.2018.6752 Text en Copyright: © Yan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yan, Liang Ma, Jinzhu Wang, Yi Zan, Jiawei Wang, Zhen Zhu, Yu Zhu, Yiping Ling, Liefeng Cao, Long Liu, Xin Li, Shu Xu, Lei Qi, Zhilin Nie, Liuwang Zhang, Yao miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells |
title | miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells |
title_full | miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells |
title_fullStr | miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells |
title_full_unstemmed | miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells |
title_short | miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells |
title_sort | mir-21-5p induces cell proliferation by targeting tgfbi in non-small cell lung cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257667/ https://www.ncbi.nlm.nih.gov/pubmed/30542417 http://dx.doi.org/10.3892/etm.2018.6752 |
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