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The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells

In this paper, the protective effects of the active fraction (SF-7) from Shaofu Zhuyu decoction (SFZYD) were tested on a hydrogen peroxide (H(2)O(2))-induced rat vascular smooth muscle cells (VSMCs) oxidative injury model. This active fraction (SF-7) shows potent antioxidant properties. The cell via...

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Autores principales: Liu, Li, Duan, Jin Ao, Tang, Yu Ping, Ma, Hong Yue, Su, Shu Lan, Guo, Jian Ming, Hua, Yong Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257770/
https://www.ncbi.nlm.nih.gov/pubmed/20714285
http://dx.doi.org/10.3390/molecules15085066
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author Liu, Li
Duan, Jin Ao
Tang, Yu Ping
Ma, Hong Yue
Su, Shu Lan
Guo, Jian Ming
Hua, Yong Qing
author_facet Liu, Li
Duan, Jin Ao
Tang, Yu Ping
Ma, Hong Yue
Su, Shu Lan
Guo, Jian Ming
Hua, Yong Qing
author_sort Liu, Li
collection PubMed
description In this paper, the protective effects of the active fraction (SF-7) from Shaofu Zhuyu decoction (SFZYD) were tested on a hydrogen peroxide (H(2)O(2))-induced rat vascular smooth muscle cells (VSMCs) oxidative injury model. This active fraction (SF-7) shows potent antioxidant properties. The cell viability and oxidative damage markers of VSMCs were determined after exposure to H(2)O(2) for 16 hours. It was observed that SF-7 significantly increased cell survival and reduced apoptosis of H(2)O(2)-injured VSMCs. Moreover, SF-7 could markedly increase intracellular superoxide dismutase (SOD) activity and decrease the malondialdehyde (MDA) level in H(2)O(2)-injured VSMCs, and suppress the generation of intracellular reactive oxygen species (ROS), as well as intracellular Ca(2+) concentration. Thus, SF-7 exhibits protective effects against H(2)O(2)-injury on VSMCs, which may be associated with its antioxidant properties. It is suggested that SF may be useful in the treatment of blood stasis syndrome in which oxidative injury is mainly implicated.
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spelling pubmed-62577702018-12-06 The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells Liu, Li Duan, Jin Ao Tang, Yu Ping Ma, Hong Yue Su, Shu Lan Guo, Jian Ming Hua, Yong Qing Molecules Article In this paper, the protective effects of the active fraction (SF-7) from Shaofu Zhuyu decoction (SFZYD) were tested on a hydrogen peroxide (H(2)O(2))-induced rat vascular smooth muscle cells (VSMCs) oxidative injury model. This active fraction (SF-7) shows potent antioxidant properties. The cell viability and oxidative damage markers of VSMCs were determined after exposure to H(2)O(2) for 16 hours. It was observed that SF-7 significantly increased cell survival and reduced apoptosis of H(2)O(2)-injured VSMCs. Moreover, SF-7 could markedly increase intracellular superoxide dismutase (SOD) activity and decrease the malondialdehyde (MDA) level in H(2)O(2)-injured VSMCs, and suppress the generation of intracellular reactive oxygen species (ROS), as well as intracellular Ca(2+) concentration. Thus, SF-7 exhibits protective effects against H(2)O(2)-injury on VSMCs, which may be associated with its antioxidant properties. It is suggested that SF may be useful in the treatment of blood stasis syndrome in which oxidative injury is mainly implicated. MDPI 2010-07-26 /pmc/articles/PMC6257770/ /pubmed/20714285 http://dx.doi.org/10.3390/molecules15085066 Text en © 2010 by the authors; http://creativecommons.org/licenses/by/3.0/ licensee MDPI, Basel, Switzerland. This article is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liu, Li
Duan, Jin Ao
Tang, Yu Ping
Ma, Hong Yue
Su, Shu Lan
Guo, Jian Ming
Hua, Yong Qing
The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells
title The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells
title_full The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells
title_fullStr The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells
title_full_unstemmed The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells
title_short The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells
title_sort protective effects of the active fraction of shaofu zhuyu decoction on hydrogen peroxide-induced oxidative injury in vascular smooth muscle cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257770/
https://www.ncbi.nlm.nih.gov/pubmed/20714285
http://dx.doi.org/10.3390/molecules15085066
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