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Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections
The most severe infections are invasive infections, due to the fact that the germs can accumulate in multiple sites and produce a body-wide infection known as sepsis. Septic shock has the highest mortality rate among non-traumatic medical conditions. In this study, we aimed to evaluate the incidence...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257814/ https://www.ncbi.nlm.nih.gov/pubmed/30542398 http://dx.doi.org/10.3892/etm.2018.6737 |
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author | Zlatian, Ovidiu Balasoiu, Andrei Theodor Balasoiu, Maria Cristea, Oana Docea, Anca Oana Mitrut, Radu Spandidos, Demetrios A. Tsatsakis, Aristides M. Bancescu, Gabriela Calina, Daniela |
author_facet | Zlatian, Ovidiu Balasoiu, Andrei Theodor Balasoiu, Maria Cristea, Oana Docea, Anca Oana Mitrut, Radu Spandidos, Demetrios A. Tsatsakis, Aristides M. Bancescu, Gabriela Calina, Daniela |
author_sort | Zlatian, Ovidiu |
collection | PubMed |
description | The most severe infections are invasive infections, due to the fact that the germs can accumulate in multiple sites and produce a body-wide infection known as sepsis. Septic shock has the highest mortality rate among non-traumatic medical conditions. In this study, we aimed to evaluate the incidence and prevalence of invasive infections in a hospital environment. Another second objective was to establish the aetiology of invasive infections in our hospital and the antibiotic resistance profile of the germs involved, which are both important for determining the therapeutic approach for the treatment of these infections. The study included 505 hospitalized patients from which we collected a total of 974 blood cultures. For the analysis of the blood cultures, we used an automated incubator. The bottles flagged as positive were subcultured on blood agar, and the grown colonies were identified using an identification system. Invasive infections had a prevalence rate of 27.72% in our hospital. From the 974 blood cultures, we isolated 170 bacterial strains: Staphylococcus aureus (SA; 63 strains, 37.06%), Klebsiella spp. (27 strains, 15.88%), coagulase-negative staphylococci (CoNS; 18 strains, 10.59%), Enterococcus spp. (17 strains, 10.00%), Escherichia coli (12 strains, 7.06%), Streptococcus spp. (11 strains, 6.47%) and other bacterial species. The prevalence of methicillin-resistant SA (MRSA) in our study was 36.51% from the SA strains. The MRSA prevalence differed significantly according to age (37.50% in adults vs. 28.57% in children, P=0.047) and ICU admission (42.42% in ICU patients vs. 30.00% in non-ICU patients, P=0.018). We performed a multivariate analysis of the invasive infection risk which detected as significant predictors the admission into the cardiology ward and plastic surgery ward. On the whole, the findings of this study indicate that the high prevalence of Gram-positive cocci in blood cultures, mostly SA, with multidrug resistance has important consequences for the management of invasive infections. |
format | Online Article Text |
id | pubmed-6257814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-62578142018-12-12 Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections Zlatian, Ovidiu Balasoiu, Andrei Theodor Balasoiu, Maria Cristea, Oana Docea, Anca Oana Mitrut, Radu Spandidos, Demetrios A. Tsatsakis, Aristides M. Bancescu, Gabriela Calina, Daniela Exp Ther Med Articles The most severe infections are invasive infections, due to the fact that the germs can accumulate in multiple sites and produce a body-wide infection known as sepsis. Septic shock has the highest mortality rate among non-traumatic medical conditions. In this study, we aimed to evaluate the incidence and prevalence of invasive infections in a hospital environment. Another second objective was to establish the aetiology of invasive infections in our hospital and the antibiotic resistance profile of the germs involved, which are both important for determining the therapeutic approach for the treatment of these infections. The study included 505 hospitalized patients from which we collected a total of 974 blood cultures. For the analysis of the blood cultures, we used an automated incubator. The bottles flagged as positive were subcultured on blood agar, and the grown colonies were identified using an identification system. Invasive infections had a prevalence rate of 27.72% in our hospital. From the 974 blood cultures, we isolated 170 bacterial strains: Staphylococcus aureus (SA; 63 strains, 37.06%), Klebsiella spp. (27 strains, 15.88%), coagulase-negative staphylococci (CoNS; 18 strains, 10.59%), Enterococcus spp. (17 strains, 10.00%), Escherichia coli (12 strains, 7.06%), Streptococcus spp. (11 strains, 6.47%) and other bacterial species. The prevalence of methicillin-resistant SA (MRSA) in our study was 36.51% from the SA strains. The MRSA prevalence differed significantly according to age (37.50% in adults vs. 28.57% in children, P=0.047) and ICU admission (42.42% in ICU patients vs. 30.00% in non-ICU patients, P=0.018). We performed a multivariate analysis of the invasive infection risk which detected as significant predictors the admission into the cardiology ward and plastic surgery ward. On the whole, the findings of this study indicate that the high prevalence of Gram-positive cocci in blood cultures, mostly SA, with multidrug resistance has important consequences for the management of invasive infections. D.A. Spandidos 2018-12 2018-09-14 /pmc/articles/PMC6257814/ /pubmed/30542398 http://dx.doi.org/10.3892/etm.2018.6737 Text en Copyright: © Zlatian et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zlatian, Ovidiu Balasoiu, Andrei Theodor Balasoiu, Maria Cristea, Oana Docea, Anca Oana Mitrut, Radu Spandidos, Demetrios A. Tsatsakis, Aristides M. Bancescu, Gabriela Calina, Daniela Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
title | Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
title_full | Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
title_fullStr | Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
title_full_unstemmed | Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
title_short | Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
title_sort | antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257814/ https://www.ncbi.nlm.nih.gov/pubmed/30542398 http://dx.doi.org/10.3892/etm.2018.6737 |
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