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Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa

OBJECTIVE: Oral squamous cell carcinoma (OSCC) invades and metastasizes, by degrading the extracellular matrix (ECM) and is associated with poor prognosis. Matrix-metalloproteinase (MMP-9) can initiate ECM degradation and angiogenesis which brings a significant change in tumor microenvironment favor...

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Autores principales: Jose, Deepa, Mane, Deepa Rajesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Qassim Uninversity 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257871/
https://www.ncbi.nlm.nih.gov/pubmed/30534042
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author Jose, Deepa
Mane, Deepa Rajesh
author_facet Jose, Deepa
Mane, Deepa Rajesh
author_sort Jose, Deepa
collection PubMed
description OBJECTIVE: Oral squamous cell carcinoma (OSCC) invades and metastasizes, by degrading the extracellular matrix (ECM) and is associated with poor prognosis. Matrix-metalloproteinase (MMP-9) can initiate ECM degradation and angiogenesis which brings a significant change in tumor microenvironment favoring tumor progression. A major thrust has been laid on understanding this key enzyme as it has significant implications for cancer therapy. Comprehending the association of vasculature with MMP-9 expression in precancerous lesions oral epithelial dysplasia (OED) and OSCC is essential since the data regarding the same are fewer. The aim is to evaluate and correlate MMP-9 expression with morphometric analysis of mucosal vasculature in the normal oral mucosa (NOM), OED, and OSCC. METHODS: A total of 60 histologically diagnosed cases of OED (n = 30); OSCC (n = 30); along with 10 NOM (n = 10) as control were included. Immunohistochemical staining of MMP-9 and vascular morphometric analysis was performed for all the cases. Results were analyzed using the Chi-square test, Fischer exact test, and Spearman correlation test. RESULTS: A statistically significant difference in MMP-9 was noted among the groups with P = 0.011* (epithelium); P = 0.001* (stroma) by the highest value in OSCC group. Morphometry also revealed a progressive increase from NOM to OED to OSCC. Spearman’s correlation of MMP-9 with vascular parameters illustrated a positive relation of MMP-9 with mean vascular density (MVD) and mean vascular area percentage (MVAP). CONCLUSION: Positive correlation of MMP-9 with MVD and MVAP demonstrates this markers effect on angiogenesis. Henceforth, MMP-9 can be embattled as a potential therapeutic target in combating tumor progression.
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spelling pubmed-62578712018-12-10 Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa Jose, Deepa Mane, Deepa Rajesh Int J Health Sci (Qassim) Original Research Type OBJECTIVE: Oral squamous cell carcinoma (OSCC) invades and metastasizes, by degrading the extracellular matrix (ECM) and is associated with poor prognosis. Matrix-metalloproteinase (MMP-9) can initiate ECM degradation and angiogenesis which brings a significant change in tumor microenvironment favoring tumor progression. A major thrust has been laid on understanding this key enzyme as it has significant implications for cancer therapy. Comprehending the association of vasculature with MMP-9 expression in precancerous lesions oral epithelial dysplasia (OED) and OSCC is essential since the data regarding the same are fewer. The aim is to evaluate and correlate MMP-9 expression with morphometric analysis of mucosal vasculature in the normal oral mucosa (NOM), OED, and OSCC. METHODS: A total of 60 histologically diagnosed cases of OED (n = 30); OSCC (n = 30); along with 10 NOM (n = 10) as control were included. Immunohistochemical staining of MMP-9 and vascular morphometric analysis was performed for all the cases. Results were analyzed using the Chi-square test, Fischer exact test, and Spearman correlation test. RESULTS: A statistically significant difference in MMP-9 was noted among the groups with P = 0.011* (epithelium); P = 0.001* (stroma) by the highest value in OSCC group. Morphometry also revealed a progressive increase from NOM to OED to OSCC. Spearman’s correlation of MMP-9 with vascular parameters illustrated a positive relation of MMP-9 with mean vascular density (MVD) and mean vascular area percentage (MVAP). CONCLUSION: Positive correlation of MMP-9 with MVD and MVAP demonstrates this markers effect on angiogenesis. Henceforth, MMP-9 can be embattled as a potential therapeutic target in combating tumor progression. Qassim Uninversity 2018 /pmc/articles/PMC6257871/ /pubmed/30534042 Text en Copyright: © International Journal of Health Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Type
Jose, Deepa
Mane, Deepa Rajesh
Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
title Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
title_full Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
title_fullStr Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
title_full_unstemmed Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
title_short Correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
title_sort correlation of matrix metalloproteinase-9 expression with morphometric analysis of mucosal vasculature in oral squamous cell carcinoma, oral epithelial dysplasia, and normal oral mucosa
topic Original Research Type
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257871/
https://www.ncbi.nlm.nih.gov/pubmed/30534042
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