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High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers
BACKGROUND: Homologous transformation sterility-1 (HTS-1) is a novel wheat mutant that exhibits pistillody, the transformation of stamens into pistils or pistil-like structures. More extreme phenotypes of this mutation can have six pistils or pistil-like structures without any stamens in a floret. T...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258151/ https://www.ncbi.nlm.nih.gov/pubmed/30477426 http://dx.doi.org/10.1186/s12870-018-1532-x |
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author | Yang, Qian Yang, Zaijun Tang, Haifeng Yu, Yan Chen, Zhenyong Wei, Shuhong Sun, Qinxu Peng, Zhengsong |
author_facet | Yang, Qian Yang, Zaijun Tang, Haifeng Yu, Yan Chen, Zhenyong Wei, Shuhong Sun, Qinxu Peng, Zhengsong |
author_sort | Yang, Qian |
collection | PubMed |
description | BACKGROUND: Homologous transformation sterility-1 (HTS-1) is a novel wheat mutant that exhibits pistillody, the transformation of stamens into pistils or pistil-like structures. More extreme phenotypes of this mutation can have six pistils or pistil-like structures without any stamens in a floret. Thus, HTS-1 is highly valuable for studies of wheat hybrid breeding and flower development. Previous studies have shown that two major genes (Pis1 and hts) control pistillody in HTS-1. The Pis1 gene controls the three-pistil trait in the three-pistil wheat mutant and has been mapped on chromosome 2D, but the hts gene has not been mapped or identified. To do so, we crossed HTS-1 with CM28TP (three-pistil mutant) and constructed a high-density linkage map with the F(2) population (200 individuals). RESULTS: The map covered 2779.96 cM, and the genetic distance per chromosome ranged from 37.59 cM to 318.95 cM. The average distance between markers was 1.04 cM. We then mapped hts between GBS-SNP markers 4A_109 and 4A_119, separated by 2.0 cM and 5.2 Mb. To find the candidate genes, the hts region was enlarged to 7.2 Mb, encompassing 752 protein-coding genes. We identified TaWin1 as a possible candidate gene after comparing the 752 genes with 206 common differentially expressed genes between pistillody stamens (PS) versus normal stamens (S) and pistils (P) versus S. Real-time PCR indicated that TaWin1 was highly expressed in HTS-1 during the pistil-and-stamen-differentiating stage, at levels approximately 120 times greater than those in CM28TP. Further analysis indicated that TaWin1 was mainly expressed in HTS-1 PS, supporting its status as a candidate gene of hts. Thus, TaWin1 overexpression probably leads to the transformation of stamens into pistils in wheat. CONCLUSIONS: The results of this study provide a foundation for further research on stamen and pistil development, with implications for wheat-hybrid breeding programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1532-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6258151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62581512018-11-29 High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers Yang, Qian Yang, Zaijun Tang, Haifeng Yu, Yan Chen, Zhenyong Wei, Shuhong Sun, Qinxu Peng, Zhengsong BMC Plant Biol Research Article BACKGROUND: Homologous transformation sterility-1 (HTS-1) is a novel wheat mutant that exhibits pistillody, the transformation of stamens into pistils or pistil-like structures. More extreme phenotypes of this mutation can have six pistils or pistil-like structures without any stamens in a floret. Thus, HTS-1 is highly valuable for studies of wheat hybrid breeding and flower development. Previous studies have shown that two major genes (Pis1 and hts) control pistillody in HTS-1. The Pis1 gene controls the three-pistil trait in the three-pistil wheat mutant and has been mapped on chromosome 2D, but the hts gene has not been mapped or identified. To do so, we crossed HTS-1 with CM28TP (three-pistil mutant) and constructed a high-density linkage map with the F(2) population (200 individuals). RESULTS: The map covered 2779.96 cM, and the genetic distance per chromosome ranged from 37.59 cM to 318.95 cM. The average distance between markers was 1.04 cM. We then mapped hts between GBS-SNP markers 4A_109 and 4A_119, separated by 2.0 cM and 5.2 Mb. To find the candidate genes, the hts region was enlarged to 7.2 Mb, encompassing 752 protein-coding genes. We identified TaWin1 as a possible candidate gene after comparing the 752 genes with 206 common differentially expressed genes between pistillody stamens (PS) versus normal stamens (S) and pistils (P) versus S. Real-time PCR indicated that TaWin1 was highly expressed in HTS-1 during the pistil-and-stamen-differentiating stage, at levels approximately 120 times greater than those in CM28TP. Further analysis indicated that TaWin1 was mainly expressed in HTS-1 PS, supporting its status as a candidate gene of hts. Thus, TaWin1 overexpression probably leads to the transformation of stamens into pistils in wheat. CONCLUSIONS: The results of this study provide a foundation for further research on stamen and pistil development, with implications for wheat-hybrid breeding programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1532-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-26 /pmc/articles/PMC6258151/ /pubmed/30477426 http://dx.doi.org/10.1186/s12870-018-1532-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yang, Qian Yang, Zaijun Tang, Haifeng Yu, Yan Chen, Zhenyong Wei, Shuhong Sun, Qinxu Peng, Zhengsong High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers |
title | High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers |
title_full | High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers |
title_fullStr | High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers |
title_full_unstemmed | High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers |
title_short | High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers |
title_sort | high-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using gbs markers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258151/ https://www.ncbi.nlm.nih.gov/pubmed/30477426 http://dx.doi.org/10.1186/s12870-018-1532-x |
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