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Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation
Elg1, the major subunit of a Replication Factor C-like complex, is critical to ensure genomic stability during DNA replication, and is implicated in controlling chromatin structure. We investigated the consequences of Elg1 loss for the dynamics of chromatin re-formation following DNA replication. Me...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258251/ https://www.ncbi.nlm.nih.gov/pubmed/30418970 http://dx.doi.org/10.1371/journal.pgen.1007783 |
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author | Gali, Vamsi K. Dickerson, David Katou, Yuki Fujiki, Katsunori Shirahige, Katsuhiko Owen-Hughes, Tom Kubota, Takashi Donaldson, Anne D. |
author_facet | Gali, Vamsi K. Dickerson, David Katou, Yuki Fujiki, Katsunori Shirahige, Katsuhiko Owen-Hughes, Tom Kubota, Takashi Donaldson, Anne D. |
author_sort | Gali, Vamsi K. |
collection | PubMed |
description | Elg1, the major subunit of a Replication Factor C-like complex, is critical to ensure genomic stability during DNA replication, and is implicated in controlling chromatin structure. We investigated the consequences of Elg1 loss for the dynamics of chromatin re-formation following DNA replication. Measurement of Okazaki fragment length and the micrococcal nuclease sensitivity of newly replicated DNA revealed a defect in nucleosome organization in the absence of Elg1. Using a proteomic approach to identify Elg1 binding partners, we discovered that Elg1 interacts with Rtt106, a histone chaperone implicated in replication-coupled nucleosome assembly that also regulates transcription. A central role for Elg1 is the unloading of PCNA from chromatin following DNA replication, so we examined the relative importance of Rtt106 and PCNA unloading for chromatin reassembly following DNA replication. We find that the major cause of the chromatin organization defects of an ELG1 mutant is PCNA retention on DNA following replication, with Rtt106-Elg1 interaction potentially playing a contributory role. |
format | Online Article Text |
id | pubmed-6258251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62582512018-12-06 Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation Gali, Vamsi K. Dickerson, David Katou, Yuki Fujiki, Katsunori Shirahige, Katsuhiko Owen-Hughes, Tom Kubota, Takashi Donaldson, Anne D. PLoS Genet Research Article Elg1, the major subunit of a Replication Factor C-like complex, is critical to ensure genomic stability during DNA replication, and is implicated in controlling chromatin structure. We investigated the consequences of Elg1 loss for the dynamics of chromatin re-formation following DNA replication. Measurement of Okazaki fragment length and the micrococcal nuclease sensitivity of newly replicated DNA revealed a defect in nucleosome organization in the absence of Elg1. Using a proteomic approach to identify Elg1 binding partners, we discovered that Elg1 interacts with Rtt106, a histone chaperone implicated in replication-coupled nucleosome assembly that also regulates transcription. A central role for Elg1 is the unloading of PCNA from chromatin following DNA replication, so we examined the relative importance of Rtt106 and PCNA unloading for chromatin reassembly following DNA replication. We find that the major cause of the chromatin organization defects of an ELG1 mutant is PCNA retention on DNA following replication, with Rtt106-Elg1 interaction potentially playing a contributory role. Public Library of Science 2018-11-12 /pmc/articles/PMC6258251/ /pubmed/30418970 http://dx.doi.org/10.1371/journal.pgen.1007783 Text en © 2018 Gali et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gali, Vamsi K. Dickerson, David Katou, Yuki Fujiki, Katsunori Shirahige, Katsuhiko Owen-Hughes, Tom Kubota, Takashi Donaldson, Anne D. Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation |
title | Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation |
title_full | Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation |
title_fullStr | Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation |
title_full_unstemmed | Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation |
title_short | Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation |
title_sort | identification of elg1 interaction partners and effects on post-replication chromatin re-formation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258251/ https://www.ncbi.nlm.nih.gov/pubmed/30418970 http://dx.doi.org/10.1371/journal.pgen.1007783 |
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