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Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar

BACKGROUND: Anthocyanins are plant secondary metabolites with key roles in attracting insect pollinators and protecting against biotic and abiotic stresses. They have potential health-promoting effects as part of the human diet. Anthocyanin biosynthesis has been elucidated in many species, enabling...

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Autores principales: Goswami, Gayatri, Nath, Ujjal Kumar, Park, Jong-In, Hossain, Mohammad Rashed, Biswas, Manosh Kumar, Kim, Hoy-Taek, Kim, Hye Ran, Nou, Ill-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258291/
https://www.ncbi.nlm.nih.gov/pubmed/30505808
http://dx.doi.org/10.1186/s40709-018-0090-6
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author Goswami, Gayatri
Nath, Ujjal Kumar
Park, Jong-In
Hossain, Mohammad Rashed
Biswas, Manosh Kumar
Kim, Hoy-Taek
Kim, Hye Ran
Nou, Ill-Sup
author_facet Goswami, Gayatri
Nath, Ujjal Kumar
Park, Jong-In
Hossain, Mohammad Rashed
Biswas, Manosh Kumar
Kim, Hoy-Taek
Kim, Hye Ran
Nou, Ill-Sup
author_sort Goswami, Gayatri
collection PubMed
description BACKGROUND: Anthocyanins are plant secondary metabolites with key roles in attracting insect pollinators and protecting against biotic and abiotic stresses. They have potential health-promoting effects as part of the human diet. Anthocyanin biosynthesis has been elucidated in many species, enabling the development of anthocyanin-enriched fruits, vegetables, and grains; however, few studies have investigated Brassica napus anthocyanin biosynthesis. RESULTS: We developed a high-anthocyanin resynthesized B. napus line, Rs035, by crossing anthocyanin-rich B. rapa (A genome) and B. oleracea (C genome) lines, followed by chromosome doubling. We identified and characterized 73 and 58 anthocyanin biosynthesis genes in silico in the A and C genomes, respectively; these genes showed syntenic relationships with 41 genes in Arabidopsis thaliana and B. napus. Among the syntenic genes, twelve biosynthetic and six regulatory genes showed transgressively higher expression in Rs035, and eight structural genes and one regulatory gene showed additive expression. We identified three early-, four late-biosynthesis pathways, three transcriptional regulator genes, and one transporter as putative candidates enhancing anthocyanin accumulation in Rs035. Principal component analysis and Pearson’s correlation coefficients corroborated the contribution of these genes to anthocyanin accumulation. CONCLUSIONS: Our study lays the foundation for producing high-anthocyanin B. napus cultivars. The resynthesized lines and the differentially expressed genes we have identified could be used to transfer the anthocyanin traits to other commercial rapeseed lines using molecular and conventional breeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40709-018-0090-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-62582912018-11-30 Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar Goswami, Gayatri Nath, Ujjal Kumar Park, Jong-In Hossain, Mohammad Rashed Biswas, Manosh Kumar Kim, Hoy-Taek Kim, Hye Ran Nou, Ill-Sup J Biol Res (Thessalon) Research BACKGROUND: Anthocyanins are plant secondary metabolites with key roles in attracting insect pollinators and protecting against biotic and abiotic stresses. They have potential health-promoting effects as part of the human diet. Anthocyanin biosynthesis has been elucidated in many species, enabling the development of anthocyanin-enriched fruits, vegetables, and grains; however, few studies have investigated Brassica napus anthocyanin biosynthesis. RESULTS: We developed a high-anthocyanin resynthesized B. napus line, Rs035, by crossing anthocyanin-rich B. rapa (A genome) and B. oleracea (C genome) lines, followed by chromosome doubling. We identified and characterized 73 and 58 anthocyanin biosynthesis genes in silico in the A and C genomes, respectively; these genes showed syntenic relationships with 41 genes in Arabidopsis thaliana and B. napus. Among the syntenic genes, twelve biosynthetic and six regulatory genes showed transgressively higher expression in Rs035, and eight structural genes and one regulatory gene showed additive expression. We identified three early-, four late-biosynthesis pathways, three transcriptional regulator genes, and one transporter as putative candidates enhancing anthocyanin accumulation in Rs035. Principal component analysis and Pearson’s correlation coefficients corroborated the contribution of these genes to anthocyanin accumulation. CONCLUSIONS: Our study lays the foundation for producing high-anthocyanin B. napus cultivars. The resynthesized lines and the differentially expressed genes we have identified could be used to transfer the anthocyanin traits to other commercial rapeseed lines using molecular and conventional breeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40709-018-0090-6) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-26 /pmc/articles/PMC6258291/ /pubmed/30505808 http://dx.doi.org/10.1186/s40709-018-0090-6 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Goswami, Gayatri
Nath, Ujjal Kumar
Park, Jong-In
Hossain, Mohammad Rashed
Biswas, Manosh Kumar
Kim, Hoy-Taek
Kim, Hye Ran
Nou, Ill-Sup
Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar
title Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar
title_full Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar
title_fullStr Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar
title_full_unstemmed Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar
title_short Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar
title_sort transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized brassica napus cultivar
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258291/
https://www.ncbi.nlm.nih.gov/pubmed/30505808
http://dx.doi.org/10.1186/s40709-018-0090-6
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