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The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice

Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are prove...

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Autores principales: Zhang, Chao, Shen, Yi, Tang, Ding, Shi, Wenqing, Zhang, Dongmei, Du, Guijie, Zhou, Yihua, Liang, Guohua, Li, Yafei, Cheng, Zhukuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258382/
https://www.ncbi.nlm.nih.gov/pubmed/30419020
http://dx.doi.org/10.1371/journal.pgen.1007769
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author Zhang, Chao
Shen, Yi
Tang, Ding
Shi, Wenqing
Zhang, Dongmei
Du, Guijie
Zhou, Yihua
Liang, Guohua
Li, Yafei
Cheng, Zhukuan
author_facet Zhang, Chao
Shen, Yi
Tang, Ding
Shi, Wenqing
Zhang, Dongmei
Du, Guijie
Zhou, Yihua
Liang, Guohua
Li, Yafei
Cheng, Zhukuan
author_sort Zhang, Chao
collection PubMed
description Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are proved to be defective in exine formation. Meiotic cytokinesis is disrupted in dcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect in dcm1 is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding the dcm1 pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified in dcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice.
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spelling pubmed-62583822018-12-06 The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice Zhang, Chao Shen, Yi Tang, Ding Shi, Wenqing Zhang, Dongmei Du, Guijie Zhou, Yihua Liang, Guohua Li, Yafei Cheng, Zhukuan PLoS Genet Research Article Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are proved to be defective in exine formation. Meiotic cytokinesis is disrupted in dcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect in dcm1 is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding the dcm1 pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified in dcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice. Public Library of Science 2018-11-12 /pmc/articles/PMC6258382/ /pubmed/30419020 http://dx.doi.org/10.1371/journal.pgen.1007769 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Chao
Shen, Yi
Tang, Ding
Shi, Wenqing
Zhang, Dongmei
Du, Guijie
Zhou, Yihua
Liang, Guohua
Li, Yafei
Cheng, Zhukuan
The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
title The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
title_full The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
title_fullStr The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
title_full_unstemmed The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
title_short The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
title_sort zinc finger protein dcm1 is required for male meiotic cytokinesis by preserving callose in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258382/
https://www.ncbi.nlm.nih.gov/pubmed/30419020
http://dx.doi.org/10.1371/journal.pgen.1007769
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