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The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice
Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are prove...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258382/ https://www.ncbi.nlm.nih.gov/pubmed/30419020 http://dx.doi.org/10.1371/journal.pgen.1007769 |
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author | Zhang, Chao Shen, Yi Tang, Ding Shi, Wenqing Zhang, Dongmei Du, Guijie Zhou, Yihua Liang, Guohua Li, Yafei Cheng, Zhukuan |
author_facet | Zhang, Chao Shen, Yi Tang, Ding Shi, Wenqing Zhang, Dongmei Du, Guijie Zhou, Yihua Liang, Guohua Li, Yafei Cheng, Zhukuan |
author_sort | Zhang, Chao |
collection | PubMed |
description | Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are proved to be defective in exine formation. Meiotic cytokinesis is disrupted in dcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect in dcm1 is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding the dcm1 pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified in dcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice. |
format | Online Article Text |
id | pubmed-6258382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62583822018-12-06 The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice Zhang, Chao Shen, Yi Tang, Ding Shi, Wenqing Zhang, Dongmei Du, Guijie Zhou, Yihua Liang, Guohua Li, Yafei Cheng, Zhukuan PLoS Genet Research Article Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are proved to be defective in exine formation. Meiotic cytokinesis is disrupted in dcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect in dcm1 is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding the dcm1 pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified in dcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice. Public Library of Science 2018-11-12 /pmc/articles/PMC6258382/ /pubmed/30419020 http://dx.doi.org/10.1371/journal.pgen.1007769 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Chao Shen, Yi Tang, Ding Shi, Wenqing Zhang, Dongmei Du, Guijie Zhou, Yihua Liang, Guohua Li, Yafei Cheng, Zhukuan The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice |
title | The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice |
title_full | The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice |
title_fullStr | The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice |
title_full_unstemmed | The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice |
title_short | The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice |
title_sort | zinc finger protein dcm1 is required for male meiotic cytokinesis by preserving callose in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258382/ https://www.ncbi.nlm.nih.gov/pubmed/30419020 http://dx.doi.org/10.1371/journal.pgen.1007769 |
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