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Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016)
Wild aquatic birds are the major reservoir of influenza A virus. Cloacal swabs and feces samples (n = 6595) were collected from 62 bird species in Argentina from 2006 to 2016 and screened for influenza A virus. Full genome sequencing of 15 influenza isolates from 6 waterfowl species revealed subtype...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258671/ https://www.ncbi.nlm.nih.gov/pubmed/30482896 http://dx.doi.org/10.1038/s41426-018-0190-2 |
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author | Rimondi, Agustina Gonzalez-Reiche, Ana S. Olivera, Valeria S. Decarre, Julieta Castresana, Gabriel J. Romano, Marcelo Nelson, Martha I. van Bakel, Harm Pereda, Ariel J. Ferreri, Lucas Geiger, Ginger Perez, Daniel R. |
author_facet | Rimondi, Agustina Gonzalez-Reiche, Ana S. Olivera, Valeria S. Decarre, Julieta Castresana, Gabriel J. Romano, Marcelo Nelson, Martha I. van Bakel, Harm Pereda, Ariel J. Ferreri, Lucas Geiger, Ginger Perez, Daniel R. |
author_sort | Rimondi, Agustina |
collection | PubMed |
description | Wild aquatic birds are the major reservoir of influenza A virus. Cloacal swabs and feces samples (n = 6595) were collected from 62 bird species in Argentina from 2006 to 2016 and screened for influenza A virus. Full genome sequencing of 15 influenza isolates from 6 waterfowl species revealed subtypes combinations that were previously described in South America (H1N1, H4N2, H4N6 (n = 3), H5N3, H6N2 (n = 4), and H10N7 (n = 2)), and new ones not previously identified in the region (H4N8, H7N7 and H7N9). Notably, the internal gene segments of all 15 Argentine isolates belonged to the South American lineage, showing a divergent evolution of these viruses in the Southern Hemisphere. Time-scaled phylogenies indicated that South American gene segments diverged between ~ 30 and ~ 140 years ago from the most closely related influenza lineages, which include the avian North American (PB1, HA, NA, MP, and NS-B) and Eurasian lineage (PB2), and the equine H3N8 lineage (PA, NP, and NS-A). Phylogenetic analyses of the hemagglutinin and neuraminidase gene segments of the H4, H6, and N8 subtypes revealed recent introductions and reassortment between viruses from the Northern and Southern Hemispheres in the Americas. Remarkably and despite evidence of recent hemagglutinin and neuraminidase subtype introductions, the phylogenetic composition of internal gene constellation of these influenza A viruses has remained unchanged. Considering the extended time and the number of sampled species of the current study, and the paucity of previously available data, our results contribute to a better understanding of the ecology and evolution of influenza virus in South America. |
format | Online Article Text |
id | pubmed-6258671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62586712018-12-03 Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) Rimondi, Agustina Gonzalez-Reiche, Ana S. Olivera, Valeria S. Decarre, Julieta Castresana, Gabriel J. Romano, Marcelo Nelson, Martha I. van Bakel, Harm Pereda, Ariel J. Ferreri, Lucas Geiger, Ginger Perez, Daniel R. Emerg Microbes Infect Article Wild aquatic birds are the major reservoir of influenza A virus. Cloacal swabs and feces samples (n = 6595) were collected from 62 bird species in Argentina from 2006 to 2016 and screened for influenza A virus. Full genome sequencing of 15 influenza isolates from 6 waterfowl species revealed subtypes combinations that were previously described in South America (H1N1, H4N2, H4N6 (n = 3), H5N3, H6N2 (n = 4), and H10N7 (n = 2)), and new ones not previously identified in the region (H4N8, H7N7 and H7N9). Notably, the internal gene segments of all 15 Argentine isolates belonged to the South American lineage, showing a divergent evolution of these viruses in the Southern Hemisphere. Time-scaled phylogenies indicated that South American gene segments diverged between ~ 30 and ~ 140 years ago from the most closely related influenza lineages, which include the avian North American (PB1, HA, NA, MP, and NS-B) and Eurasian lineage (PB2), and the equine H3N8 lineage (PA, NP, and NS-A). Phylogenetic analyses of the hemagglutinin and neuraminidase gene segments of the H4, H6, and N8 subtypes revealed recent introductions and reassortment between viruses from the Northern and Southern Hemispheres in the Americas. Remarkably and despite evidence of recent hemagglutinin and neuraminidase subtype introductions, the phylogenetic composition of internal gene constellation of these influenza A viruses has remained unchanged. Considering the extended time and the number of sampled species of the current study, and the paucity of previously available data, our results contribute to a better understanding of the ecology and evolution of influenza virus in South America. Nature Publishing Group UK 2018-11-28 /pmc/articles/PMC6258671/ /pubmed/30482896 http://dx.doi.org/10.1038/s41426-018-0190-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rimondi, Agustina Gonzalez-Reiche, Ana S. Olivera, Valeria S. Decarre, Julieta Castresana, Gabriel J. Romano, Marcelo Nelson, Martha I. van Bakel, Harm Pereda, Ariel J. Ferreri, Lucas Geiger, Ginger Perez, Daniel R. Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) |
title | Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) |
title_full | Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) |
title_fullStr | Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) |
title_full_unstemmed | Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) |
title_short | Evidence of a fixed internal gene constellation in influenza A viruses isolated from wild birds in Argentina (2006–2016) |
title_sort | evidence of a fixed internal gene constellation in influenza a viruses isolated from wild birds in argentina (2006–2016) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258671/ https://www.ncbi.nlm.nih.gov/pubmed/30482896 http://dx.doi.org/10.1038/s41426-018-0190-2 |
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