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Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions
Sinorhizobium fredii indigenous populations are prevalent in provinces of Central China whereas Bradyrhizobium species (Bradyrhizobium japonicum, B. diazoefficiens, B. elkanii, and others) are more abundant in northern and southern provinces. The symbiotic properties of different soybean rhizobia ha...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258812/ https://www.ncbi.nlm.nih.gov/pubmed/30519234 http://dx.doi.org/10.3389/fmicb.2018.02843 |
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author | Temprano-Vera, Francisco Rodríguez-Navarro, Dulce Nombre Acosta-Jurado, Sebastian Perret, Xavier Fossou, Romain K. Navarro-Gómez, Pilar Zhen, Tao Yu, Deshui An, Qi Buendía-Clavería, Ana Maria Moreno, Javier López-Baena, Francisco Javier Ruiz-Sainz, Jose Enrique Vinardell, Jose Maria |
author_facet | Temprano-Vera, Francisco Rodríguez-Navarro, Dulce Nombre Acosta-Jurado, Sebastian Perret, Xavier Fossou, Romain K. Navarro-Gómez, Pilar Zhen, Tao Yu, Deshui An, Qi Buendía-Clavería, Ana Maria Moreno, Javier López-Baena, Francisco Javier Ruiz-Sainz, Jose Enrique Vinardell, Jose Maria |
author_sort | Temprano-Vera, Francisco |
collection | PubMed |
description | Sinorhizobium fredii indigenous populations are prevalent in provinces of Central China whereas Bradyrhizobium species (Bradyrhizobium japonicum, B. diazoefficiens, B. elkanii, and others) are more abundant in northern and southern provinces. The symbiotic properties of different soybean rhizobia have been investigated with 40 different wild soybean (Glycine soja) accessions from China, Japan, Russia, and South Korea. Bradyrhizobial strains nodulated all the wild soybeans tested, albeit efficiency of nitrogen fixation varied considerably among accessions. The symbiotic capacity of S. fredii HH103 with wild soybeans from Central China was clearly better than with the accessions found elsewhere. S. fredii NGR234, the rhizobial strain showing the broadest host range ever described, also formed nitrogen-fixing nodules with different G. soja accessions from Central China. To our knowledge, this is the first report describing an effective symbiosis between S. fredii NGR234 and G. soja. Mobilization of the S. fredii HH103 symbiotic plasmid to a NGR234 pSym-cured derivative (strain NGR234C) yielded transconjugants that formed ineffective nodules with G. max cv. Williams 82 and G. soja accession CH4. By contrast, transfer of the symbiotic plasmid pNGR234a to a pSym(-)cured derivative of S. fredii USDA193 generated transconjugants that effectively nodulated G. soja accession CH4 but failed to nodulate with G. max cv. Williams 82. These results indicate that intra-specific transference of the S. fredii symbiotic plasmids generates new strains with unpredictable symbiotic properties, probably due to the occurrence of new combinations of symbiotic signals. |
format | Online Article Text |
id | pubmed-6258812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62588122018-12-05 Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions Temprano-Vera, Francisco Rodríguez-Navarro, Dulce Nombre Acosta-Jurado, Sebastian Perret, Xavier Fossou, Romain K. Navarro-Gómez, Pilar Zhen, Tao Yu, Deshui An, Qi Buendía-Clavería, Ana Maria Moreno, Javier López-Baena, Francisco Javier Ruiz-Sainz, Jose Enrique Vinardell, Jose Maria Front Microbiol Microbiology Sinorhizobium fredii indigenous populations are prevalent in provinces of Central China whereas Bradyrhizobium species (Bradyrhizobium japonicum, B. diazoefficiens, B. elkanii, and others) are more abundant in northern and southern provinces. The symbiotic properties of different soybean rhizobia have been investigated with 40 different wild soybean (Glycine soja) accessions from China, Japan, Russia, and South Korea. Bradyrhizobial strains nodulated all the wild soybeans tested, albeit efficiency of nitrogen fixation varied considerably among accessions. The symbiotic capacity of S. fredii HH103 with wild soybeans from Central China was clearly better than with the accessions found elsewhere. S. fredii NGR234, the rhizobial strain showing the broadest host range ever described, also formed nitrogen-fixing nodules with different G. soja accessions from Central China. To our knowledge, this is the first report describing an effective symbiosis between S. fredii NGR234 and G. soja. Mobilization of the S. fredii HH103 symbiotic plasmid to a NGR234 pSym-cured derivative (strain NGR234C) yielded transconjugants that formed ineffective nodules with G. max cv. Williams 82 and G. soja accession CH4. By contrast, transfer of the symbiotic plasmid pNGR234a to a pSym(-)cured derivative of S. fredii USDA193 generated transconjugants that effectively nodulated G. soja accession CH4 but failed to nodulate with G. max cv. Williams 82. These results indicate that intra-specific transference of the S. fredii symbiotic plasmids generates new strains with unpredictable symbiotic properties, probably due to the occurrence of new combinations of symbiotic signals. Frontiers Media S.A. 2018-11-21 /pmc/articles/PMC6258812/ /pubmed/30519234 http://dx.doi.org/10.3389/fmicb.2018.02843 Text en Copyright © 2018 Temprano-Vera, Rodríguez-Navarro, Acosta-Jurado, Perret, Fossou, Navarro-Gómez, Zhen, Yu, An, Buendía-Clavería, Moreno, López-Baena, Ruiz-Sainz and Vinardell. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Temprano-Vera, Francisco Rodríguez-Navarro, Dulce Nombre Acosta-Jurado, Sebastian Perret, Xavier Fossou, Romain K. Navarro-Gómez, Pilar Zhen, Tao Yu, Deshui An, Qi Buendía-Clavería, Ana Maria Moreno, Javier López-Baena, Francisco Javier Ruiz-Sainz, Jose Enrique Vinardell, Jose Maria Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions |
title | Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions |
title_full | Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions |
title_fullStr | Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions |
title_full_unstemmed | Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions |
title_short | Sinorhizobium fredii Strains HH103 and NGR234 Form Nitrogen Fixing Nodules With Diverse Wild Soybeans (Glycine soja) From Central China but Are Ineffective on Northern China Accessions |
title_sort | sinorhizobium fredii strains hh103 and ngr234 form nitrogen fixing nodules with diverse wild soybeans (glycine soja) from central china but are ineffective on northern china accessions |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258812/ https://www.ncbi.nlm.nih.gov/pubmed/30519234 http://dx.doi.org/10.3389/fmicb.2018.02843 |
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