Cargando…
Role of Immunophenotypes in Carcinoma Breast
BACKGROUND: Nottingham's modification of Bloom–Richardson histopathological grading system (NGS) for carcinoma breast is a time-tested prognostic indicator; however, of lately, breast cancer has been evaluated through molecular techniques, particularly assessing the gene expression profiling an...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259294/ https://www.ncbi.nlm.nih.gov/pubmed/30598906 http://dx.doi.org/10.4103/ijabmr.IJABMR_331_17 |
_version_ | 1783374653962584064 |
---|---|
author | Shukla, Samarth Acharya, Sourya Vagha, Sunita Dawande, Pratibha Tamhane, Ankita |
author_facet | Shukla, Samarth Acharya, Sourya Vagha, Sunita Dawande, Pratibha Tamhane, Ankita |
author_sort | Shukla, Samarth |
collection | PubMed |
description | BACKGROUND: Nottingham's modification of Bloom–Richardson histopathological grading system (NGS) for carcinoma breast is a time-tested prognostic indicator; however, of lately, breast cancer has been evaluated through molecular techniques, particularly assessing the gene expression profiling and establishing molecular or immunophenotypes. The present-day utility of NGS needs to be reassessed with the modern predictive markers, this may help refine breast cancer classification specifically to help improve the treatment protocol. OBJECTIVE: The objective was to compare breast cancer immunophenotypes with prognostic factors such as age (based on menstrual status), tumor size, lymph node (LN) status, also to compare the NGS grade with the molecular immunphenotypes of breast cancer. MATERIALS AND METHODS: The present work was carried out in the Histopathology and Immunohistochemistry section of Department of Pathology, of a central Indian medical college and rural hospital from January 2013 to July 2016. It was a prospective analytical study. A tota1 of 114 female patients presenting in the outpatient department of surgery with lump in breast were included in the present study. All patients underwent modified radical mastectomy for tumor resection. Tumor masses and LNs were subjected to routine hematoxylin and eosin staining as well as immunohistochemistry then examined by a senior pathologist. Comparisons were made between molecular immunophenotypes with patient age, tumor size, and LN status, further NGS grade of breast cancer was compared with immunophenotypes. RESULTS: The study found that the molecular immunophenotypes when compared with clinical prognostic parameters, i.e; age (based on menstrual status of female), LN involvement in patients of breast carcinoma showed inconsequential correlation, the tumor size showed significant correlation. However, when histopathological grades were compared with molecular immunophenotypes, a significant correlation was seen. CONCLUSION: NGS grade being an excellent predictive prognostic tool should be continued for assessing the grades in breast cancer patients. The molecular markers correlate with the histopathological grading and indirectly aid the oncologist in assessing the aggressiveness, these immunophenotypes are not helpful as suitable prognostic tools. As the molecular phenotypes definitely indicate the hormonal receptor status in breast cancer patients, they become mandatory in guiding oncologists for planning the treatment strategy and protocol. |
format | Online Article Text |
id | pubmed-6259294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-62592942018-12-31 Role of Immunophenotypes in Carcinoma Breast Shukla, Samarth Acharya, Sourya Vagha, Sunita Dawande, Pratibha Tamhane, Ankita Int J Appl Basic Med Res Original Article BACKGROUND: Nottingham's modification of Bloom–Richardson histopathological grading system (NGS) for carcinoma breast is a time-tested prognostic indicator; however, of lately, breast cancer has been evaluated through molecular techniques, particularly assessing the gene expression profiling and establishing molecular or immunophenotypes. The present-day utility of NGS needs to be reassessed with the modern predictive markers, this may help refine breast cancer classification specifically to help improve the treatment protocol. OBJECTIVE: The objective was to compare breast cancer immunophenotypes with prognostic factors such as age (based on menstrual status), tumor size, lymph node (LN) status, also to compare the NGS grade with the molecular immunphenotypes of breast cancer. MATERIALS AND METHODS: The present work was carried out in the Histopathology and Immunohistochemistry section of Department of Pathology, of a central Indian medical college and rural hospital from January 2013 to July 2016. It was a prospective analytical study. A tota1 of 114 female patients presenting in the outpatient department of surgery with lump in breast were included in the present study. All patients underwent modified radical mastectomy for tumor resection. Tumor masses and LNs were subjected to routine hematoxylin and eosin staining as well as immunohistochemistry then examined by a senior pathologist. Comparisons were made between molecular immunophenotypes with patient age, tumor size, and LN status, further NGS grade of breast cancer was compared with immunophenotypes. RESULTS: The study found that the molecular immunophenotypes when compared with clinical prognostic parameters, i.e; age (based on menstrual status of female), LN involvement in patients of breast carcinoma showed inconsequential correlation, the tumor size showed significant correlation. However, when histopathological grades were compared with molecular immunophenotypes, a significant correlation was seen. CONCLUSION: NGS grade being an excellent predictive prognostic tool should be continued for assessing the grades in breast cancer patients. The molecular markers correlate with the histopathological grading and indirectly aid the oncologist in assessing the aggressiveness, these immunophenotypes are not helpful as suitable prognostic tools. As the molecular phenotypes definitely indicate the hormonal receptor status in breast cancer patients, they become mandatory in guiding oncologists for planning the treatment strategy and protocol. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC6259294/ /pubmed/30598906 http://dx.doi.org/10.4103/ijabmr.IJABMR_331_17 Text en Copyright: © 2018 International Journal of Applied and Basic Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Shukla, Samarth Acharya, Sourya Vagha, Sunita Dawande, Pratibha Tamhane, Ankita Role of Immunophenotypes in Carcinoma Breast |
title | Role of Immunophenotypes in Carcinoma Breast |
title_full | Role of Immunophenotypes in Carcinoma Breast |
title_fullStr | Role of Immunophenotypes in Carcinoma Breast |
title_full_unstemmed | Role of Immunophenotypes in Carcinoma Breast |
title_short | Role of Immunophenotypes in Carcinoma Breast |
title_sort | role of immunophenotypes in carcinoma breast |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259294/ https://www.ncbi.nlm.nih.gov/pubmed/30598906 http://dx.doi.org/10.4103/ijabmr.IJABMR_331_17 |
work_keys_str_mv | AT shuklasamarth roleofimmunophenotypesincarcinomabreast AT acharyasourya roleofimmunophenotypesincarcinomabreast AT vaghasunita roleofimmunophenotypesincarcinomabreast AT dawandepratibha roleofimmunophenotypesincarcinomabreast AT tamhaneankita roleofimmunophenotypesincarcinomabreast |