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The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells

1’-(S)-1’-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethno-medicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MT...

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Autores principales: Awang, Khalijah, Azmi, Mohamad Nurul, Aun, Lionel In Lian, Aziz, Ahmad Nazif, Ibrahim, Halijah, Nagoor, Noor Hasima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259418/
https://www.ncbi.nlm.nih.gov/pubmed/21063268
http://dx.doi.org/10.3390/molecules15118048
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author Awang, Khalijah
Azmi, Mohamad Nurul
Aun, Lionel In Lian
Aziz, Ahmad Nazif
Ibrahim, Halijah
Nagoor, Noor Hasima
author_facet Awang, Khalijah
Azmi, Mohamad Nurul
Aun, Lionel In Lian
Aziz, Ahmad Nazif
Ibrahim, Halijah
Nagoor, Noor Hasima
author_sort Awang, Khalijah
collection PubMed
description 1’-(S)-1’-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethno-medicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time- and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 µM concentration. Flow cytometric analysis for annexin-V and PI dual staining demonstrated that cell death occurred via apoptosis, followed by secondary necrosis. The apoptotic effects of ACA were confirmed via the DNA fragmentation assay, in which consistent laddering of genomic DNA was observed for all tumour cell lines after a 24 hrs post-treatment period at the IC(50) concentration of ACA. A cell cycle analysis using PI staining also demonstrated that ACA induced cell cycle arrest at the G(0)/G(1) phase, corresponding to oral tumour cell lines. In conclusion, ACA exhibits enormous potential for future development as a chemotherapeutic drug against various malignancies.
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spelling pubmed-62594182018-12-06 The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells Awang, Khalijah Azmi, Mohamad Nurul Aun, Lionel In Lian Aziz, Ahmad Nazif Ibrahim, Halijah Nagoor, Noor Hasima Molecules Article 1’-(S)-1’-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethno-medicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time- and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 µM concentration. Flow cytometric analysis for annexin-V and PI dual staining demonstrated that cell death occurred via apoptosis, followed by secondary necrosis. The apoptotic effects of ACA were confirmed via the DNA fragmentation assay, in which consistent laddering of genomic DNA was observed for all tumour cell lines after a 24 hrs post-treatment period at the IC(50) concentration of ACA. A cell cycle analysis using PI staining also demonstrated that ACA induced cell cycle arrest at the G(0)/G(1) phase, corresponding to oral tumour cell lines. In conclusion, ACA exhibits enormous potential for future development as a chemotherapeutic drug against various malignancies. MDPI 2010-11-09 /pmc/articles/PMC6259418/ /pubmed/21063268 http://dx.doi.org/10.3390/molecules15118048 Text en © 2010 by the authors; http://creativecommons.org/licenses/by/3.0/ licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Awang, Khalijah
Azmi, Mohamad Nurul
Aun, Lionel In Lian
Aziz, Ahmad Nazif
Ibrahim, Halijah
Nagoor, Noor Hasima
The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells
title The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells
title_full The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells
title_fullStr The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells
title_full_unstemmed The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells
title_short The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells
title_sort apoptotic effect of 1’s-1’-acetoxychavicol acetate from alpinia conchigera on human cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259418/
https://www.ncbi.nlm.nih.gov/pubmed/21063268
http://dx.doi.org/10.3390/molecules15118048
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