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DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods

Peptide-based molecular probes identified by bacteriophage (phage) display technology expand the peptide repertoire for in vivo diagnosis and therapy of cancer. Numerous peptides that bind cancer-associated antigens have been discovered by panning phage libraries. However, until now only few of the...

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Autores principales: Lindner, Thomas, Kolmar, Harald, Haberkorn, Uwe, Mier, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259622/
https://www.ncbi.nlm.nih.gov/pubmed/21326140
http://dx.doi.org/10.3390/molecules16021625
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author Lindner, Thomas
Kolmar, Harald
Haberkorn, Uwe
Mier, Walter
author_facet Lindner, Thomas
Kolmar, Harald
Haberkorn, Uwe
Mier, Walter
author_sort Lindner, Thomas
collection PubMed
description Peptide-based molecular probes identified by bacteriophage (phage) display technology expand the peptide repertoire for in vivo diagnosis and therapy of cancer. Numerous peptides that bind cancer-associated antigens have been discovered by panning phage libraries. However, until now only few of the peptides selected by phage display have entered clinical applications. The success of phage derived peptides essentially depends on the quality of the library screened. This review summarizes the methods to achieve highly homogenous libraries that cover a maximal sequence space. Biochemical and chemical strategies for the synthesis of DNA libraries and the techniques for their integration into the viral genome are discussed in detail. A focus is set on the methods that enable the exclusion of disturbing sequences. In addition, the parameters that define the variability, the minimal numbers of copies per library and the use of alternating panning cycles to avoid the loss of selected hits are evaluated.
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spelling pubmed-62596222018-12-20 DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods Lindner, Thomas Kolmar, Harald Haberkorn, Uwe Mier, Walter Molecules Review Peptide-based molecular probes identified by bacteriophage (phage) display technology expand the peptide repertoire for in vivo diagnosis and therapy of cancer. Numerous peptides that bind cancer-associated antigens have been discovered by panning phage libraries. However, until now only few of the peptides selected by phage display have entered clinical applications. The success of phage derived peptides essentially depends on the quality of the library screened. This review summarizes the methods to achieve highly homogenous libraries that cover a maximal sequence space. Biochemical and chemical strategies for the synthesis of DNA libraries and the techniques for their integration into the viral genome are discussed in detail. A focus is set on the methods that enable the exclusion of disturbing sequences. In addition, the parameters that define the variability, the minimal numbers of copies per library and the use of alternating panning cycles to avoid the loss of selected hits are evaluated. MDPI 2011-02-15 /pmc/articles/PMC6259622/ /pubmed/21326140 http://dx.doi.org/10.3390/molecules16021625 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Lindner, Thomas
Kolmar, Harald
Haberkorn, Uwe
Mier, Walter
DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
title DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
title_full DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
title_fullStr DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
title_full_unstemmed DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
title_short DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
title_sort dna libraries for the construction of phage libraries: statistical and structural requirements and synthetic methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259622/
https://www.ncbi.nlm.nih.gov/pubmed/21326140
http://dx.doi.org/10.3390/molecules16021625
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