Cargando…

Miniproteins as Phage Display-Scaffolds for Clinical Applications

Miniproteins are currently developed as alternative, non-immunoglobin proteins for the generation of novel binding motifs. Miniproteins are rigid scaffolds that are stabilised by alpha-helices, beta-sheets and disulfide-constrained secondary structural elements. They are tolerant to multiple amino a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zoller, Frederic, Haberkorn, Uwe, Mier, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259850/
https://www.ncbi.nlm.nih.gov/pubmed/21407148
http://dx.doi.org/10.3390/molecules16032467
_version_ 1783374741184184320
author Zoller, Frederic
Haberkorn, Uwe
Mier, Walter
author_facet Zoller, Frederic
Haberkorn, Uwe
Mier, Walter
author_sort Zoller, Frederic
collection PubMed
description Miniproteins are currently developed as alternative, non-immunoglobin proteins for the generation of novel binding motifs. Miniproteins are rigid scaffolds that are stabilised by alpha-helices, beta-sheets and disulfide-constrained secondary structural elements. They are tolerant to multiple amino acid substitutions, which allow for the integration of a randomised affinity function into the stably folded framework. These properties classify miniprotein scaffolds as promising tools for lead structure generation using phage display technologies. Owing to their high enzymatic resistance and structural stability, miniproteins are ideal templates to display binding epitopes for medical applications in vivo. This review summarises the characteristics and the engineering of miniproteins as a novel class of scaffolds to generate of alternative binding agents using phage display screening. Moreover, recent developments for therapeutic and especially diagnostic applications of miniproteins are reviewed.
format Online
Article
Text
id pubmed-6259850
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62598502018-12-07 Miniproteins as Phage Display-Scaffolds for Clinical Applications Zoller, Frederic Haberkorn, Uwe Mier, Walter Molecules Review Miniproteins are currently developed as alternative, non-immunoglobin proteins for the generation of novel binding motifs. Miniproteins are rigid scaffolds that are stabilised by alpha-helices, beta-sheets and disulfide-constrained secondary structural elements. They are tolerant to multiple amino acid substitutions, which allow for the integration of a randomised affinity function into the stably folded framework. These properties classify miniprotein scaffolds as promising tools for lead structure generation using phage display technologies. Owing to their high enzymatic resistance and structural stability, miniproteins are ideal templates to display binding epitopes for medical applications in vivo. This review summarises the characteristics and the engineering of miniproteins as a novel class of scaffolds to generate of alternative binding agents using phage display screening. Moreover, recent developments for therapeutic and especially diagnostic applications of miniproteins are reviewed. MDPI 2011-03-14 /pmc/articles/PMC6259850/ /pubmed/21407148 http://dx.doi.org/10.3390/molecules16032467 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Zoller, Frederic
Haberkorn, Uwe
Mier, Walter
Miniproteins as Phage Display-Scaffolds for Clinical Applications
title Miniproteins as Phage Display-Scaffolds for Clinical Applications
title_full Miniproteins as Phage Display-Scaffolds for Clinical Applications
title_fullStr Miniproteins as Phage Display-Scaffolds for Clinical Applications
title_full_unstemmed Miniproteins as Phage Display-Scaffolds for Clinical Applications
title_short Miniproteins as Phage Display-Scaffolds for Clinical Applications
title_sort miniproteins as phage display-scaffolds for clinical applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259850/
https://www.ncbi.nlm.nih.gov/pubmed/21407148
http://dx.doi.org/10.3390/molecules16032467
work_keys_str_mv AT zollerfrederic miniproteinsasphagedisplayscaffoldsforclinicalapplications
AT haberkornuwe miniproteinsasphagedisplayscaffoldsforclinicalapplications
AT mierwalter miniproteinsasphagedisplayscaffoldsforclinicalapplications