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Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)

Extracts of Melittis melissophyllum leaves in ether, chloroform, ethyl acetate, n-butanol and water were evaporated to dryness and dissolved in 50% ethanol to make 10% (w/v) solutions. The potential protective action of the extracts was assessed by the corresponding in vitro and in vivo tests. In th...

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Autores principales: Kaurinovic, Biljana, Popovic, Mira, Vlaisavljevic, Sanja, Raseta, Milena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260604/
https://www.ncbi.nlm.nih.gov/pubmed/21494204
http://dx.doi.org/10.3390/molecules16043152
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author Kaurinovic, Biljana
Popovic, Mira
Vlaisavljevic, Sanja
Raseta, Milena
author_facet Kaurinovic, Biljana
Popovic, Mira
Vlaisavljevic, Sanja
Raseta, Milena
author_sort Kaurinovic, Biljana
collection PubMed
description Extracts of Melittis melissophyllum leaves in ether, chloroform, ethyl acetate, n-butanol and water were evaporated to dryness and dissolved in 50% ethanol to make 10% (w/v) solutions. The potential protective action of the extracts was assessed by the corresponding in vitro and in vivo tests. In the in vitro experiments extracts were tested as potential scavengers of free radicals (DPPH, O(2)(·)(−), NO, and OH radicals), as well as inhibitors of liposomal peroxidation (LPx). The results obtained show that all extracts (exept n-BuOH extract) are good scavengers of radicals and reduce LPx intensity in liposomes, which points to their protective (antioxidant) activity. In vivo experiments were concerned with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood-hemolysate of experimental animals after their treatment with extracts of M. melissophyllum leaves, or in combination with CCl(4). On the basis of the results obtained it can be concluded that the examined extracts have protective (antioxidative) effect and this antioxidative behaviour is more pronounced in liver than in blood-hemolysate. The reason is probably the fact that liver contains other enzymatic systems, which can also participate in the antioxidative mechanism. Of all the extracts the H(2)O one showed the highest protective activity.
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spelling pubmed-62606042018-12-10 Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae) Kaurinovic, Biljana Popovic, Mira Vlaisavljevic, Sanja Raseta, Milena Molecules Article Extracts of Melittis melissophyllum leaves in ether, chloroform, ethyl acetate, n-butanol and water were evaporated to dryness and dissolved in 50% ethanol to make 10% (w/v) solutions. The potential protective action of the extracts was assessed by the corresponding in vitro and in vivo tests. In the in vitro experiments extracts were tested as potential scavengers of free radicals (DPPH, O(2)(·)(−), NO, and OH radicals), as well as inhibitors of liposomal peroxidation (LPx). The results obtained show that all extracts (exept n-BuOH extract) are good scavengers of radicals and reduce LPx intensity in liposomes, which points to their protective (antioxidant) activity. In vivo experiments were concerned with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood-hemolysate of experimental animals after their treatment with extracts of M. melissophyllum leaves, or in combination with CCl(4). On the basis of the results obtained it can be concluded that the examined extracts have protective (antioxidative) effect and this antioxidative behaviour is more pronounced in liver than in blood-hemolysate. The reason is probably the fact that liver contains other enzymatic systems, which can also participate in the antioxidative mechanism. Of all the extracts the H(2)O one showed the highest protective activity. MDPI 2011-04-14 /pmc/articles/PMC6260604/ /pubmed/21494204 http://dx.doi.org/10.3390/molecules16043152 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kaurinovic, Biljana
Popovic, Mira
Vlaisavljevic, Sanja
Raseta, Milena
Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)
title Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)
title_full Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)
title_fullStr Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)
title_full_unstemmed Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)
title_short Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)
title_sort antioxidant activities of melittis melissophyllum l. (lamiaceae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260604/
https://www.ncbi.nlm.nih.gov/pubmed/21494204
http://dx.doi.org/10.3390/molecules16043152
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