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Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring
BACKGROUND: Foot-and-mouth disease (FMD) can be controlled by either stamping out or vaccination, a choice which depends on both the economic importance of the livestock sector as well as the disease status. In FMD-free countries with vaccination, such as Korea, vaccination programs should guarantee...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260702/ https://www.ncbi.nlm.nih.gov/pubmed/30486820 http://dx.doi.org/10.1186/s12917-018-1686-7 |
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author | Kang, Yeong-Lim Jeong, Ji-Yun Choi, Hwi-Yeon Zhang, Yanhong Liu, Yumei Lee, Ho-Jong Choi, Jong-Chul Lee, So-Hyun Lee, Beom-Joo Lee, Sang-Won Lee, Joong-Bok Cho, Ki-hyun Park, Seung-Yong |
author_facet | Kang, Yeong-Lim Jeong, Ji-Yun Choi, Hwi-Yeon Zhang, Yanhong Liu, Yumei Lee, Ho-Jong Choi, Jong-Chul Lee, So-Hyun Lee, Beom-Joo Lee, Sang-Won Lee, Joong-Bok Cho, Ki-hyun Park, Seung-Yong |
author_sort | Kang, Yeong-Lim |
collection | PubMed |
description | BACKGROUND: Foot-and-mouth disease (FMD) can be controlled by either stamping out or vaccination, a choice which depends on both the economic importance of the livestock sector as well as the disease status. In FMD-free countries with vaccination, such as Korea, vaccination programs should guarantee prevention against transmission of FMD. Monitoring of vaccination programs is also essential for ensuring sufficient coverage that will limit the transmission of FMDV. There are several methods to screen FMD virus (FMDV) structural protein (SP) antibodies including SPCE (Solid-phase competitive ELISA), LPBE (Liquid-phase blocking ELISA), and VNT (Virus neutralization test). Among these, SPCE is widely used for serological monitoring since VNT—the gold standard method—has certain practical limitations, such as high costs in terms of time and labor. However, whether SPCE can ensure the vaccination status of individual animals and whole farms is unclear. In this study, SPCE, LPBE and VNT were compared with respect to correlation with each other and sensitivity at commercial pig farms. RESULTS: The positive results obtained by PrioCHECK SPCE differed from those obtained by LPBE and VNT. The sensitivity of SPCE relative to those of the other tests was fairly low. The raw data of SPCE were most highly correlated with those of VNT with XJ strain, while their positivity and negativity were most highly correlated with LPBE. The results of ROC analysis proposed new cut-off for PrioCHECK SPCE higher than the previous 50% inhibition. CONCLUSIONS: The high false positive rate of PrioCHECK SPCE suggested that high seropositivity by SPCE may not guarantee a true vaccination coverage. Adjusting the cut-off percentage (%) inhibition value for SPCE is needed to address this problem, and it is highly recommended that routine FMDV serological monitoring programs using PrioCHECK SPCE should be combined with alternative methods such as LPBE or VNT. |
format | Online Article Text |
id | pubmed-6260702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62607022018-11-30 Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring Kang, Yeong-Lim Jeong, Ji-Yun Choi, Hwi-Yeon Zhang, Yanhong Liu, Yumei Lee, Ho-Jong Choi, Jong-Chul Lee, So-Hyun Lee, Beom-Joo Lee, Sang-Won Lee, Joong-Bok Cho, Ki-hyun Park, Seung-Yong BMC Vet Res Research Article BACKGROUND: Foot-and-mouth disease (FMD) can be controlled by either stamping out or vaccination, a choice which depends on both the economic importance of the livestock sector as well as the disease status. In FMD-free countries with vaccination, such as Korea, vaccination programs should guarantee prevention against transmission of FMD. Monitoring of vaccination programs is also essential for ensuring sufficient coverage that will limit the transmission of FMDV. There are several methods to screen FMD virus (FMDV) structural protein (SP) antibodies including SPCE (Solid-phase competitive ELISA), LPBE (Liquid-phase blocking ELISA), and VNT (Virus neutralization test). Among these, SPCE is widely used for serological monitoring since VNT—the gold standard method—has certain practical limitations, such as high costs in terms of time and labor. However, whether SPCE can ensure the vaccination status of individual animals and whole farms is unclear. In this study, SPCE, LPBE and VNT were compared with respect to correlation with each other and sensitivity at commercial pig farms. RESULTS: The positive results obtained by PrioCHECK SPCE differed from those obtained by LPBE and VNT. The sensitivity of SPCE relative to those of the other tests was fairly low. The raw data of SPCE were most highly correlated with those of VNT with XJ strain, while their positivity and negativity were most highly correlated with LPBE. The results of ROC analysis proposed new cut-off for PrioCHECK SPCE higher than the previous 50% inhibition. CONCLUSIONS: The high false positive rate of PrioCHECK SPCE suggested that high seropositivity by SPCE may not guarantee a true vaccination coverage. Adjusting the cut-off percentage (%) inhibition value for SPCE is needed to address this problem, and it is highly recommended that routine FMDV serological monitoring programs using PrioCHECK SPCE should be combined with alternative methods such as LPBE or VNT. BioMed Central 2018-11-28 /pmc/articles/PMC6260702/ /pubmed/30486820 http://dx.doi.org/10.1186/s12917-018-1686-7 Text en © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Kang, Yeong-Lim Jeong, Ji-Yun Choi, Hwi-Yeon Zhang, Yanhong Liu, Yumei Lee, Ho-Jong Choi, Jong-Chul Lee, So-Hyun Lee, Beom-Joo Lee, Sang-Won Lee, Joong-Bok Cho, Ki-hyun Park, Seung-Yong Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring |
title | Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring |
title_full | Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring |
title_fullStr | Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring |
title_full_unstemmed | Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring |
title_short | Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring |
title_sort | evaluation and optimization of a conventional spce for fmd post-vaccination monitoring |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260702/ https://www.ncbi.nlm.nih.gov/pubmed/30486820 http://dx.doi.org/10.1186/s12917-018-1686-7 |
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