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Antivenomic approach of different Crotalus durissus collilineatus venoms
BACKGROUND: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this w...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260869/ https://www.ncbi.nlm.nih.gov/pubmed/30534148 http://dx.doi.org/10.1186/s40409-018-0169-4 |
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author | Oliveira, Isadora Sousa de Pucca, Manuela Berto Sampaio, Suely Vilela Arantes, Eliane Candiani |
author_facet | Oliveira, Isadora Sousa de Pucca, Manuela Berto Sampaio, Suely Vilela Arantes, Eliane Candiani |
author_sort | Oliveira, Isadora Sousa de |
collection | PubMed |
description | BACKGROUND: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian anticrotalic serum capacity in recognizing twenty-two Crotalus durissus collilineatus venoms, as well as their fractions. METHODS: The indirect enzyme-linked immunosorbent assay (ELISA) was chosen to evaluate the efficacy of heterologous anticrotalic serum produced by Instituto Butantan (Brazil) in recognizing the twenty-two Crotalus durissus collilineatus venoms and the pool of them. Moreover, the venom pool was fractionated using reversed-phase fast protein liquid chromatography (RP-FPLC) and the obtained fractions were analyzed concerning antivenom recognition. RESULTS: Evaluation of venom variability by ELISA showed that all venom samples were recognized by the Brazilian anticrotalic antivenom. However, some particular venom fractions were poorly recognized. CONCLUSION: This study demonstrated that the Brazilian anticrotalic serum recognizes all the different twenty-two venoms of C. d. collilineatus and their fractions, although in a quantitatively different way, which may impact the effectiveness of the antivenom therapy. These results confirm the need to use a pool of venoms with the greatest possible variability in the preparation of antivenoms, in order to improve their effectiveness. |
format | Online Article Text |
id | pubmed-6260869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62608692018-12-10 Antivenomic approach of different Crotalus durissus collilineatus venoms Oliveira, Isadora Sousa de Pucca, Manuela Berto Sampaio, Suely Vilela Arantes, Eliane Candiani J Venom Anim Toxins Incl Trop Dis Short Report BACKGROUND: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian anticrotalic serum capacity in recognizing twenty-two Crotalus durissus collilineatus venoms, as well as their fractions. METHODS: The indirect enzyme-linked immunosorbent assay (ELISA) was chosen to evaluate the efficacy of heterologous anticrotalic serum produced by Instituto Butantan (Brazil) in recognizing the twenty-two Crotalus durissus collilineatus venoms and the pool of them. Moreover, the venom pool was fractionated using reversed-phase fast protein liquid chromatography (RP-FPLC) and the obtained fractions were analyzed concerning antivenom recognition. RESULTS: Evaluation of venom variability by ELISA showed that all venom samples were recognized by the Brazilian anticrotalic antivenom. However, some particular venom fractions were poorly recognized. CONCLUSION: This study demonstrated that the Brazilian anticrotalic serum recognizes all the different twenty-two venoms of C. d. collilineatus and their fractions, although in a quantitatively different way, which may impact the effectiveness of the antivenom therapy. These results confirm the need to use a pool of venoms with the greatest possible variability in the preparation of antivenoms, in order to improve their effectiveness. BioMed Central 2018-11-26 /pmc/articles/PMC6260869/ /pubmed/30534148 http://dx.doi.org/10.1186/s40409-018-0169-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Oliveira, Isadora Sousa de Pucca, Manuela Berto Sampaio, Suely Vilela Arantes, Eliane Candiani Antivenomic approach of different Crotalus durissus collilineatus venoms |
title | Antivenomic approach of different Crotalus durissus collilineatus venoms |
title_full | Antivenomic approach of different Crotalus durissus collilineatus venoms |
title_fullStr | Antivenomic approach of different Crotalus durissus collilineatus venoms |
title_full_unstemmed | Antivenomic approach of different Crotalus durissus collilineatus venoms |
title_short | Antivenomic approach of different Crotalus durissus collilineatus venoms |
title_sort | antivenomic approach of different crotalus durissus collilineatus venoms |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260869/ https://www.ncbi.nlm.nih.gov/pubmed/30534148 http://dx.doi.org/10.1186/s40409-018-0169-4 |
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