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Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses

OBJECTIVES: To use next generation sequencing to characterize the microbiota of horses during healing of skin wounds in two anatomical locations (body and limb) known to present different healing patterns; and to investigate the impact of bandaging on bacterial communities of skin wounds located on...

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Autores principales: Kamus, Louis J., Theoret, Christine, Costa, Marcio C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261015/
https://www.ncbi.nlm.nih.gov/pubmed/30475922
http://dx.doi.org/10.1371/journal.pone.0206989
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author Kamus, Louis J.
Theoret, Christine
Costa, Marcio C.
author_facet Kamus, Louis J.
Theoret, Christine
Costa, Marcio C.
author_sort Kamus, Louis J.
collection PubMed
description OBJECTIVES: To use next generation sequencing to characterize the microbiota of horses during healing of skin wounds in two anatomical locations (body and limb) known to present different healing patterns; and to investigate the impact of bandaging on bacterial communities of skin wounds located on the limbs of horses. METHODS: Full-thickness skin wounds were created on the distal extremity of both thoracic limbs and on one lateral mid-thoracic wall of four healthy horses. Limb wounds were randomly assigned to bandaging or not. A full-thickness sample was collected with a biopsy punch from intact thorax and limb skin (T0) and from the margin of one wound per site (thorax, unbandaged limb, bandaged limb) 1 week (T1) and 2 weeks (T2) postoperatively, and at full healing (T3). Thoracic skin samples obtained from three healthy horses were included in the analysis as controls. RESULTS: Anatomic location (thorax vs. limb) significantly influenced bacterial composition of equine skin and healing wounds. Fusobacterium and Actinobacillus were strongly associated with limb wounds during the initial phases of healing. Bandaging had a significant impact on the microbiota during the healing process. The skin microbiota after healing was more similar to samples from controls, demonstrating the resilience and stability of the environment. CONCLUSIONS: Equine skin microbiota is a rich and stable environment that is disturbed by wounding, but returns to its previous stage after full healing. Anatomic location significantly influences bacterial composition of the equine skin during wound healing. Bandaging has a significant impact on the skin microbiota of horses during the healing process. Results of this study provide new insight for a better understanding of the contribution of bacteria to wound healing in horses and may facilitate the future development of therapeutic strategies using commensal bacteria.
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spelling pubmed-62610152018-12-06 Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses Kamus, Louis J. Theoret, Christine Costa, Marcio C. PLoS One Research Article OBJECTIVES: To use next generation sequencing to characterize the microbiota of horses during healing of skin wounds in two anatomical locations (body and limb) known to present different healing patterns; and to investigate the impact of bandaging on bacterial communities of skin wounds located on the limbs of horses. METHODS: Full-thickness skin wounds were created on the distal extremity of both thoracic limbs and on one lateral mid-thoracic wall of four healthy horses. Limb wounds were randomly assigned to bandaging or not. A full-thickness sample was collected with a biopsy punch from intact thorax and limb skin (T0) and from the margin of one wound per site (thorax, unbandaged limb, bandaged limb) 1 week (T1) and 2 weeks (T2) postoperatively, and at full healing (T3). Thoracic skin samples obtained from three healthy horses were included in the analysis as controls. RESULTS: Anatomic location (thorax vs. limb) significantly influenced bacterial composition of equine skin and healing wounds. Fusobacterium and Actinobacillus were strongly associated with limb wounds during the initial phases of healing. Bandaging had a significant impact on the microbiota during the healing process. The skin microbiota after healing was more similar to samples from controls, demonstrating the resilience and stability of the environment. CONCLUSIONS: Equine skin microbiota is a rich and stable environment that is disturbed by wounding, but returns to its previous stage after full healing. Anatomic location significantly influences bacterial composition of the equine skin during wound healing. Bandaging has a significant impact on the skin microbiota of horses during the healing process. Results of this study provide new insight for a better understanding of the contribution of bacteria to wound healing in horses and may facilitate the future development of therapeutic strategies using commensal bacteria. Public Library of Science 2018-11-26 /pmc/articles/PMC6261015/ /pubmed/30475922 http://dx.doi.org/10.1371/journal.pone.0206989 Text en © 2018 Kamus et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kamus, Louis J.
Theoret, Christine
Costa, Marcio C.
Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
title Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
title_full Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
title_fullStr Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
title_full_unstemmed Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
title_short Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
title_sort use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261015/
https://www.ncbi.nlm.nih.gov/pubmed/30475922
http://dx.doi.org/10.1371/journal.pone.0206989
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