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A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance

In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field (1)H Nuclear magnetic resonance (LF‐(1)H NMR). The experimental parameters of LF‐(1)H NMR have been optimized and the validity of the established standard method has been confirmed with tr...

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Detalles Bibliográficos
Autores principales: Zhang, Hongcai, Yu, Huaning, Song, Lingling, Chen, Shunsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261215/
https://www.ncbi.nlm.nih.gov/pubmed/30510700
http://dx.doi.org/10.1002/fsn3.699
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author Zhang, Hongcai
Yu, Huaning
Song, Lingling
Chen, Shunsheng
author_facet Zhang, Hongcai
Yu, Huaning
Song, Lingling
Chen, Shunsheng
author_sort Zhang, Hongcai
collection PubMed
description In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field (1)H Nuclear magnetic resonance (LF‐(1)H NMR). The experimental parameters of LF‐(1)H NMR have been optimized and the validity of the established standard method has been confirmed with traditional Soxhlet extraction method. Results show that the lipid signal intensity is strongly correlated with its content and exhibits a good linear correlation (Y = 0.0376 + 4.899X, R (2) = 0.9999), thus demonstrating favorable accuracy and sensitivity for the quantitative determination of lipid content. In conclusion, the lipid content of live E. Sinensis can be directly obtained based on an established method, indicating a great application potential in food and other fields.
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spelling pubmed-62612152018-12-03 A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance Zhang, Hongcai Yu, Huaning Song, Lingling Chen, Shunsheng Food Sci Nutr Method In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field (1)H Nuclear magnetic resonance (LF‐(1)H NMR). The experimental parameters of LF‐(1)H NMR have been optimized and the validity of the established standard method has been confirmed with traditional Soxhlet extraction method. Results show that the lipid signal intensity is strongly correlated with its content and exhibits a good linear correlation (Y = 0.0376 + 4.899X, R (2) = 0.9999), thus demonstrating favorable accuracy and sensitivity for the quantitative determination of lipid content. In conclusion, the lipid content of live E. Sinensis can be directly obtained based on an established method, indicating a great application potential in food and other fields. John Wiley and Sons Inc. 2018-09-27 /pmc/articles/PMC6261215/ /pubmed/30510700 http://dx.doi.org/10.1002/fsn3.699 Text en © 2018 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Zhang, Hongcai
Yu, Huaning
Song, Lingling
Chen, Shunsheng
A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
title A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
title_full A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
title_fullStr A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
title_full_unstemmed A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
title_short A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
title_sort novel, sensitive and non‐destructive method for quantitative determination of lipid in live eriocheir sinensis using low‐field (1)h nuclear magnetic resonance
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261215/
https://www.ncbi.nlm.nih.gov/pubmed/30510700
http://dx.doi.org/10.1002/fsn3.699
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