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A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance
In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field (1)H Nuclear magnetic resonance (LF‐(1)H NMR). The experimental parameters of LF‐(1)H NMR have been optimized and the validity of the established standard method has been confirmed with tr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261215/ https://www.ncbi.nlm.nih.gov/pubmed/30510700 http://dx.doi.org/10.1002/fsn3.699 |
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author | Zhang, Hongcai Yu, Huaning Song, Lingling Chen, Shunsheng |
author_facet | Zhang, Hongcai Yu, Huaning Song, Lingling Chen, Shunsheng |
author_sort | Zhang, Hongcai |
collection | PubMed |
description | In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field (1)H Nuclear magnetic resonance (LF‐(1)H NMR). The experimental parameters of LF‐(1)H NMR have been optimized and the validity of the established standard method has been confirmed with traditional Soxhlet extraction method. Results show that the lipid signal intensity is strongly correlated with its content and exhibits a good linear correlation (Y = 0.0376 + 4.899X, R (2) = 0.9999), thus demonstrating favorable accuracy and sensitivity for the quantitative determination of lipid content. In conclusion, the lipid content of live E. Sinensis can be directly obtained based on an established method, indicating a great application potential in food and other fields. |
format | Online Article Text |
id | pubmed-6261215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62612152018-12-03 A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance Zhang, Hongcai Yu, Huaning Song, Lingling Chen, Shunsheng Food Sci Nutr Method In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field (1)H Nuclear magnetic resonance (LF‐(1)H NMR). The experimental parameters of LF‐(1)H NMR have been optimized and the validity of the established standard method has been confirmed with traditional Soxhlet extraction method. Results show that the lipid signal intensity is strongly correlated with its content and exhibits a good linear correlation (Y = 0.0376 + 4.899X, R (2) = 0.9999), thus demonstrating favorable accuracy and sensitivity for the quantitative determination of lipid content. In conclusion, the lipid content of live E. Sinensis can be directly obtained based on an established method, indicating a great application potential in food and other fields. John Wiley and Sons Inc. 2018-09-27 /pmc/articles/PMC6261215/ /pubmed/30510700 http://dx.doi.org/10.1002/fsn3.699 Text en © 2018 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Method Zhang, Hongcai Yu, Huaning Song, Lingling Chen, Shunsheng A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance |
title | A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance |
title_full | A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance |
title_fullStr | A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance |
title_full_unstemmed | A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance |
title_short | A novel, sensitive and non‐destructive method for quantitative determination of lipid in live Eriocheir sinensis using low‐field (1)H Nuclear magnetic resonance |
title_sort | novel, sensitive and non‐destructive method for quantitative determination of lipid in live eriocheir sinensis using low‐field (1)h nuclear magnetic resonance |
topic | Method |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261215/ https://www.ncbi.nlm.nih.gov/pubmed/30510700 http://dx.doi.org/10.1002/fsn3.699 |
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