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Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs
The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261828/ https://www.ncbi.nlm.nih.gov/pubmed/30282841 http://dx.doi.org/10.1292/jvms.18-0132 |
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author | KOIKE, Naoki MAI, Thi Ngan SHIRAI, Mamoru KUBO, Meiko HATA, Kazuhiro MARUMOTO, Nobuyuki WATANABE, Shinji SASAKI, Yosuke MITOMA, Shuya NOTSU, Kosuke OKABAYASHI, Tamaki WIRATSUDAKUL, Anuwat KABALI, Emmanuel NORIMINE, Junzo SEKIGUCHI, Satoshi |
author_facet | KOIKE, Naoki MAI, Thi Ngan SHIRAI, Mamoru KUBO, Meiko HATA, Kazuhiro MARUMOTO, Nobuyuki WATANABE, Shinji SASAKI, Yosuke MITOMA, Shuya NOTSU, Kosuke OKABAYASHI, Tamaki WIRATSUDAKUL, Anuwat KABALI, Emmanuel NORIMINE, Junzo SEKIGUCHI, Satoshi |
author_sort | KOIKE, Naoki |
collection | PubMed |
description | The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, P<0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (P<0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment. |
format | Online Article Text |
id | pubmed-6261828 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62618282018-12-04 Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs KOIKE, Naoki MAI, Thi Ngan SHIRAI, Mamoru KUBO, Meiko HATA, Kazuhiro MARUMOTO, Nobuyuki WATANABE, Shinji SASAKI, Yosuke MITOMA, Shuya NOTSU, Kosuke OKABAYASHI, Tamaki WIRATSUDAKUL, Anuwat KABALI, Emmanuel NORIMINE, Junzo SEKIGUCHI, Satoshi J Vet Med Sci Virology The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, P<0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (P<0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment. The Japanese Society of Veterinary Science 2018-10-02 2018-11 /pmc/articles/PMC6261828/ /pubmed/30282841 http://dx.doi.org/10.1292/jvms.18-0132 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Virology KOIKE, Naoki MAI, Thi Ngan SHIRAI, Mamoru KUBO, Meiko HATA, Kazuhiro MARUMOTO, Nobuyuki WATANABE, Shinji SASAKI, Yosuke MITOMA, Shuya NOTSU, Kosuke OKABAYASHI, Tamaki WIRATSUDAKUL, Anuwat KABALI, Emmanuel NORIMINE, Junzo SEKIGUCHI, Satoshi Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
title | Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
title_full | Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
title_fullStr | Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
title_full_unstemmed | Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
title_short | Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
title_sort | detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261828/ https://www.ncbi.nlm.nih.gov/pubmed/30282841 http://dx.doi.org/10.1292/jvms.18-0132 |
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