Dynamic and non-contact 3D sample rotation for microscopy

Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for fre...

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Autores principales: Berndt, Frederic, Shah, Gopi, Power, Rory M., Brugués, Jan, Huisken, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261998/
https://www.ncbi.nlm.nih.gov/pubmed/30487638
http://dx.doi.org/10.1038/s41467-018-07504-3
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author Berndt, Frederic
Shah, Gopi
Power, Rory M.
Brugués, Jan
Huisken, Jan
author_facet Berndt, Frederic
Shah, Gopi
Power, Rory M.
Brugués, Jan
Huisken, Jan
author_sort Berndt, Frederic
collection PubMed
description Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.
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spelling pubmed-62619982018-11-30 Dynamic and non-contact 3D sample rotation for microscopy Berndt, Frederic Shah, Gopi Power, Rory M. Brugués, Jan Huisken, Jan Nat Commun Article Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging. Nature Publishing Group UK 2018-11-28 /pmc/articles/PMC6261998/ /pubmed/30487638 http://dx.doi.org/10.1038/s41467-018-07504-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Berndt, Frederic
Shah, Gopi
Power, Rory M.
Brugués, Jan
Huisken, Jan
Dynamic and non-contact 3D sample rotation for microscopy
title Dynamic and non-contact 3D sample rotation for microscopy
title_full Dynamic and non-contact 3D sample rotation for microscopy
title_fullStr Dynamic and non-contact 3D sample rotation for microscopy
title_full_unstemmed Dynamic and non-contact 3D sample rotation for microscopy
title_short Dynamic and non-contact 3D sample rotation for microscopy
title_sort dynamic and non-contact 3d sample rotation for microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261998/
https://www.ncbi.nlm.nih.gov/pubmed/30487638
http://dx.doi.org/10.1038/s41467-018-07504-3
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