Cargando…
The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces
The xenobiotic response element (XRE) transcription factors belong to a regulator family frequently found in Streptomyces that are often followed by small proteins with a DUF397 domain. In fact, the pair XRE-DUF397 has been proposed to comprise toxin–antitoxin (TA) type II systems. In this work, we...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262351/ https://www.ncbi.nlm.nih.gov/pubmed/30524403 http://dx.doi.org/10.3389/fmicb.2018.02791 |
_version_ | 1783375085754646528 |
---|---|
author | Santamaría, Ramón I. Sevillano, Laura Martín, Jesús Genilloud, Olga González, Ignacio Díaz, Margarita |
author_facet | Santamaría, Ramón I. Sevillano, Laura Martín, Jesús Genilloud, Olga González, Ignacio Díaz, Margarita |
author_sort | Santamaría, Ramón I. |
collection | PubMed |
description | The xenobiotic response element (XRE) transcription factors belong to a regulator family frequently found in Streptomyces that are often followed by small proteins with a DUF397 domain. In fact, the pair XRE-DUF397 has been proposed to comprise toxin–antitoxin (TA) type II systems. In this work, we demonstrate that one of these putative TA-systems, encoded by the genes SCO4441 and SCO4442 of Streptomyces coelicolor, and denominated Scr1/Scr2 (which stands for S. coelicolor regulator), does not behave as a toxin–antitoxin system under the conditions used as was originally expected. Instead the pair Scr1/Scr2 acts as a strong positive regulator of endogenous antibiotic production in S. coelicolor. The analysis of the 19 Streptomyces strains tested determined that overexpression of the pair Scr1/Scr2 drastically induces the production of antibiotics not only in S. coelicolor, but also in Streptomyces lividans, Streptomyces peucetius, Streptomyces steffisburgensis and Streptomyces sp. CA-240608. Our work also shows that Scr1 needs Scr2 to exert positive regulation on antibiotic production. |
format | Online Article Text |
id | pubmed-6262351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62623512018-12-06 The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces Santamaría, Ramón I. Sevillano, Laura Martín, Jesús Genilloud, Olga González, Ignacio Díaz, Margarita Front Microbiol Microbiology The xenobiotic response element (XRE) transcription factors belong to a regulator family frequently found in Streptomyces that are often followed by small proteins with a DUF397 domain. In fact, the pair XRE-DUF397 has been proposed to comprise toxin–antitoxin (TA) type II systems. In this work, we demonstrate that one of these putative TA-systems, encoded by the genes SCO4441 and SCO4442 of Streptomyces coelicolor, and denominated Scr1/Scr2 (which stands for S. coelicolor regulator), does not behave as a toxin–antitoxin system under the conditions used as was originally expected. Instead the pair Scr1/Scr2 acts as a strong positive regulator of endogenous antibiotic production in S. coelicolor. The analysis of the 19 Streptomyces strains tested determined that overexpression of the pair Scr1/Scr2 drastically induces the production of antibiotics not only in S. coelicolor, but also in Streptomyces lividans, Streptomyces peucetius, Streptomyces steffisburgensis and Streptomyces sp. CA-240608. Our work also shows that Scr1 needs Scr2 to exert positive regulation on antibiotic production. Frontiers Media S.A. 2018-11-16 /pmc/articles/PMC6262351/ /pubmed/30524403 http://dx.doi.org/10.3389/fmicb.2018.02791 Text en Copyright © 2018 Santamaría, Sevillano, Martín, Genilloud, González and Díaz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Santamaría, Ramón I. Sevillano, Laura Martín, Jesús Genilloud, Olga González, Ignacio Díaz, Margarita The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces |
title | The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces |
title_full | The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces |
title_fullStr | The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces |
title_full_unstemmed | The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces |
title_short | The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces |
title_sort | xre-duf397 protein pair, scr1 and scr2, acts as a strong positive regulator of antibiotic production in streptomyces |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262351/ https://www.ncbi.nlm.nih.gov/pubmed/30524403 http://dx.doi.org/10.3389/fmicb.2018.02791 |
work_keys_str_mv | AT santamariaramoni thexreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT sevillanolaura thexreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT martinjesus thexreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT genilloudolga thexreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT gonzalezignacio thexreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT diazmargarita thexreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT santamariaramoni xreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT sevillanolaura xreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT martinjesus xreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT genilloudolga xreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT gonzalezignacio xreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces AT diazmargarita xreduf397proteinpairscr1andscr2actsasastrongpositiveregulatorofantibioticproductioninstreptomyces |