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Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line

Myxofibrosarcoma (MFS) belongs to the group of sarcoma tumors, which represent only 1% of the totality of adult tumors worldwide. Thus, given the rare nature of this cancer, this makes the availability of MFS cell lines difficult. In an attempt to partially fill this gap, we immortalized a primary c...

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Autores principales: Miserocchi, Giacomo, De Vita, Alessandro, Mercatali, Laura, Recine, Federica, Liverani, Chiara, Spadazzi, Chiara, Pieri, Federica, Riva, Nada, Bongiovanni, Alberto, Casadei, Roberto, Fausti, Valentina, Ibrahim, Toni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262427/
https://www.ncbi.nlm.nih.gov/pubmed/30366467
http://dx.doi.org/10.3390/cells7110186
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author Miserocchi, Giacomo
De Vita, Alessandro
Mercatali, Laura
Recine, Federica
Liverani, Chiara
Spadazzi, Chiara
Pieri, Federica
Riva, Nada
Bongiovanni, Alberto
Casadei, Roberto
Fausti, Valentina
Ibrahim, Toni
author_facet Miserocchi, Giacomo
De Vita, Alessandro
Mercatali, Laura
Recine, Federica
Liverani, Chiara
Spadazzi, Chiara
Pieri, Federica
Riva, Nada
Bongiovanni, Alberto
Casadei, Roberto
Fausti, Valentina
Ibrahim, Toni
author_sort Miserocchi, Giacomo
collection PubMed
description Myxofibrosarcoma (MFS) belongs to the group of sarcoma tumors, which represent only 1% of the totality of adult tumors worldwide. Thus, given the rare nature of this cancer, this makes the availability of MFS cell lines difficult. In an attempt to partially fill this gap, we immortalized a primary culture of MFS (IM-MFS-1) and compared the cell morphology with patient’s tumor tissue. IM-MFS-1 was genetically characterized through a Comparative Genomic Hybridization (CGH) array and the mesenchymal phenotype was evaluated using Polymerase chain reaction (PCR) and immunofluorescence staining. Drug sensitivity for MFS therapies was monitored over time in cultures. We confirmed the conservation of the patient’s tumor cell morphology and of the mesenchymal phenotype. Conversely, the synthesis and expression of CD109, a TGFβ co-receptor used to facilitate the diagnosis of high-grade MFS diagnosis, was maintained constant until high cancer cell line passages. The CGH array revealed a complex karyotype with cytogenetic alterations that include chromosome regions associated with genes involved in tumor processes. Cytotoxicity assays show drug sensitivity constantly increased during the culture passages until a plateau was reached. In conclusion, we established and characterized a new MFS cell line that can be used for future preclinical and molecular studies on soft tissue sarcomas.
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spelling pubmed-62624272018-12-03 Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line Miserocchi, Giacomo De Vita, Alessandro Mercatali, Laura Recine, Federica Liverani, Chiara Spadazzi, Chiara Pieri, Federica Riva, Nada Bongiovanni, Alberto Casadei, Roberto Fausti, Valentina Ibrahim, Toni Cells Article Myxofibrosarcoma (MFS) belongs to the group of sarcoma tumors, which represent only 1% of the totality of adult tumors worldwide. Thus, given the rare nature of this cancer, this makes the availability of MFS cell lines difficult. In an attempt to partially fill this gap, we immortalized a primary culture of MFS (IM-MFS-1) and compared the cell morphology with patient’s tumor tissue. IM-MFS-1 was genetically characterized through a Comparative Genomic Hybridization (CGH) array and the mesenchymal phenotype was evaluated using Polymerase chain reaction (PCR) and immunofluorescence staining. Drug sensitivity for MFS therapies was monitored over time in cultures. We confirmed the conservation of the patient’s tumor cell morphology and of the mesenchymal phenotype. Conversely, the synthesis and expression of CD109, a TGFβ co-receptor used to facilitate the diagnosis of high-grade MFS diagnosis, was maintained constant until high cancer cell line passages. The CGH array revealed a complex karyotype with cytogenetic alterations that include chromosome regions associated with genes involved in tumor processes. Cytotoxicity assays show drug sensitivity constantly increased during the culture passages until a plateau was reached. In conclusion, we established and characterized a new MFS cell line that can be used for future preclinical and molecular studies on soft tissue sarcomas. MDPI 2018-10-25 /pmc/articles/PMC6262427/ /pubmed/30366467 http://dx.doi.org/10.3390/cells7110186 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miserocchi, Giacomo
De Vita, Alessandro
Mercatali, Laura
Recine, Federica
Liverani, Chiara
Spadazzi, Chiara
Pieri, Federica
Riva, Nada
Bongiovanni, Alberto
Casadei, Roberto
Fausti, Valentina
Ibrahim, Toni
Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line
title Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line
title_full Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line
title_fullStr Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line
title_full_unstemmed Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line
title_short Characterization and Drug Sensitivity of a New High-Grade Myxofibrosarcoma Cell Line
title_sort characterization and drug sensitivity of a new high-grade myxofibrosarcoma cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262427/
https://www.ncbi.nlm.nih.gov/pubmed/30366467
http://dx.doi.org/10.3390/cells7110186
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