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Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development
Photoreceptor cells (PRCs) mature from simple epithelial cells, a process characterised by growth and compartmentalisation of the apical membrane into an inner and an outer segment. So far, a PRC subtype-specific description of morphological and cellular changes in the developing zebrafish retina is...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262866/ https://www.ncbi.nlm.nih.gov/pubmed/30237290 http://dx.doi.org/10.1242/bio.036632 |
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author | Crespo, Cátia Knust, Elisabeth |
author_facet | Crespo, Cátia Knust, Elisabeth |
author_sort | Crespo, Cátia |
collection | PubMed |
description | Photoreceptor cells (PRCs) mature from simple epithelial cells, a process characterised by growth and compartmentalisation of the apical membrane into an inner and an outer segment. So far, a PRC subtype-specific description of morphological and cellular changes in the developing zebrafish retina is missing. Here, we performed an in-depth characterisation of four of the five PRC subtypes of the zebrafish retina between 51 and 120 h post fertilisation, including quantification of the size of different compartments, localisation of polarity proteins and positioning of organelles. One of the major findings was the anisotropic and subtype-specific growth of the different PRC compartments. In addition, a transient accumulation of endoplasmic reticulum in rod PRCs, changes in chromatin organisation in UV sensitive cones and differential expression of polarity proteins during the initial stages of PRC maturation were observed. The results obtained provide a developmental timeline that can be used as a platform for future studies on PRC maturation and function. This platform was applied to document that increased exposure to light leads to smaller apical domains of PRCs. |
format | Online Article Text |
id | pubmed-6262866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-62628662018-11-30 Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development Crespo, Cátia Knust, Elisabeth Biol Open Research Article Photoreceptor cells (PRCs) mature from simple epithelial cells, a process characterised by growth and compartmentalisation of the apical membrane into an inner and an outer segment. So far, a PRC subtype-specific description of morphological and cellular changes in the developing zebrafish retina is missing. Here, we performed an in-depth characterisation of four of the five PRC subtypes of the zebrafish retina between 51 and 120 h post fertilisation, including quantification of the size of different compartments, localisation of polarity proteins and positioning of organelles. One of the major findings was the anisotropic and subtype-specific growth of the different PRC compartments. In addition, a transient accumulation of endoplasmic reticulum in rod PRCs, changes in chromatin organisation in UV sensitive cones and differential expression of polarity proteins during the initial stages of PRC maturation were observed. The results obtained provide a developmental timeline that can be used as a platform for future studies on PRC maturation and function. This platform was applied to document that increased exposure to light leads to smaller apical domains of PRCs. The Company of Biologists Ltd 2018-09-20 /pmc/articles/PMC6262866/ /pubmed/30237290 http://dx.doi.org/10.1242/bio.036632 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Crespo, Cátia Knust, Elisabeth Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
title | Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
title_full | Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
title_fullStr | Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
title_full_unstemmed | Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
title_short | Characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
title_sort | characterisation of maturation of photoreceptor cell subtypes during zebrafish retinal development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262866/ https://www.ncbi.nlm.nih.gov/pubmed/30237290 http://dx.doi.org/10.1242/bio.036632 |
work_keys_str_mv | AT crespocatia characterisationofmaturationofphotoreceptorcellsubtypesduringzebrafishretinaldevelopment AT knustelisabeth characterisationofmaturationofphotoreceptorcellsubtypesduringzebrafishretinaldevelopment |