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Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty
PURPOSE: To optimize methods of preparing donor cornea tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK), we compared five experimental conditions with different internal pressures and cutting systems. METHODS: The artificial anterior chamber internal pressure (IP) was...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262888/ https://www.ncbi.nlm.nih.gov/pubmed/30510855 http://dx.doi.org/10.1167/tvst.7.6.11 |
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author | Nishisako, Sota Aoki, Dai Sasaki, Chiaki Higa, Kazunari Shimazaki, Jun |
author_facet | Nishisako, Sota Aoki, Dai Sasaki, Chiaki Higa, Kazunari Shimazaki, Jun |
author_sort | Nishisako, Sota |
collection | PubMed |
description | PURPOSE: To optimize methods of preparing donor cornea tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK), we compared five experimental conditions with different internal pressures and cutting systems. METHODS: The artificial anterior chamber internal pressure (IP) was set at 100 or 200 mm Hg. The microkeratome cut was performed with or without an artificial chamber pressurizer (ACP), using a CBm turbine (CBm) or one use-plus automated (OUP-A). Thirty human research corneas were divided into five groups, and compared after the cut with donor tissue quality parameters, including cutting depth, graft uniformity, cell evaluation, and smoothness of the stromal surface. RESULTS: The smallest variation in mean cut depth was observed in the condition, which had IP of 200 mm Hg used ACP and OUP-A. In experimental groups cut using CBm, significantly more consistent thicknesses were made at an IP of 200 than 100 mm Hg. There were no statistically significant differences among the groups in either endothelial cell density or cell viable assay results after cuts. Using an IP of 200 mm Hg with ACP and CBm produced the roughest stromal surface, and the roughness grading scores showed a positive correlation with the percentage of cut depth. CONCLUSIONS: An IP of 200 mm Hg was the best setting for DSAEK grafts with high predictability of cut depth and uniformity of graft thickness without endothelial cell damage. TRANSLATIONAL RELEVANCE: For successful DSAEK, it is recommended that a set internal pressure of 200 mm Hg be used during microkeratome cutting for donor tissue preparation. |
format | Online Article Text |
id | pubmed-6262888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-62628882018-12-03 Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty Nishisako, Sota Aoki, Dai Sasaki, Chiaki Higa, Kazunari Shimazaki, Jun Transl Vis Sci Technol Articles PURPOSE: To optimize methods of preparing donor cornea tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK), we compared five experimental conditions with different internal pressures and cutting systems. METHODS: The artificial anterior chamber internal pressure (IP) was set at 100 or 200 mm Hg. The microkeratome cut was performed with or without an artificial chamber pressurizer (ACP), using a CBm turbine (CBm) or one use-plus automated (OUP-A). Thirty human research corneas were divided into five groups, and compared after the cut with donor tissue quality parameters, including cutting depth, graft uniformity, cell evaluation, and smoothness of the stromal surface. RESULTS: The smallest variation in mean cut depth was observed in the condition, which had IP of 200 mm Hg used ACP and OUP-A. In experimental groups cut using CBm, significantly more consistent thicknesses were made at an IP of 200 than 100 mm Hg. There were no statistically significant differences among the groups in either endothelial cell density or cell viable assay results after cuts. Using an IP of 200 mm Hg with ACP and CBm produced the roughest stromal surface, and the roughness grading scores showed a positive correlation with the percentage of cut depth. CONCLUSIONS: An IP of 200 mm Hg was the best setting for DSAEK grafts with high predictability of cut depth and uniformity of graft thickness without endothelial cell damage. TRANSLATIONAL RELEVANCE: For successful DSAEK, it is recommended that a set internal pressure of 200 mm Hg be used during microkeratome cutting for donor tissue preparation. The Association for Research in Vision and Ophthalmology 2018-11-27 /pmc/articles/PMC6262888/ /pubmed/30510855 http://dx.doi.org/10.1167/tvst.7.6.11 Text en Copyright 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Articles Nishisako, Sota Aoki, Dai Sasaki, Chiaki Higa, Kazunari Shimazaki, Jun Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty |
title | Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty |
title_full | Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty |
title_fullStr | Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty |
title_full_unstemmed | Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty |
title_short | Comparison of Artificial Anterior Chamber Internal Pressures and Cutting Systems for Descemet's Stripping Automated Endothelial Keratoplasty |
title_sort | comparison of artificial anterior chamber internal pressures and cutting systems for descemet's stripping automated endothelial keratoplasty |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262888/ https://www.ncbi.nlm.nih.gov/pubmed/30510855 http://dx.doi.org/10.1167/tvst.7.6.11 |
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