Cargando…
Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon
Simian immunodeficiency virus (SIVgor) causes persistent infection in critically endangered western lowland gorillas (Gorilla gorilla gorilla) from west central Africa. SIVgor is closely related to chimpanzee and human immunodeficiency viruses (SIVcpz and HIV‐1, respectively). We established a nonin...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262910/ https://www.ncbi.nlm.nih.gov/pubmed/30519399 http://dx.doi.org/10.1002/ece3.4478 |
_version_ | 1783375195862466560 |
---|---|
author | Villabona‐Arenas, Christian Julian Ayouba, Ahidjo Esteban, Amandine D'arc, Mirela Mpoudi Ngole, Eitel Peeters, Martine |
author_facet | Villabona‐Arenas, Christian Julian Ayouba, Ahidjo Esteban, Amandine D'arc, Mirela Mpoudi Ngole, Eitel Peeters, Martine |
author_sort | Villabona‐Arenas, Christian Julian |
collection | PubMed |
description | Simian immunodeficiency virus (SIVgor) causes persistent infection in critically endangered western lowland gorillas (Gorilla gorilla gorilla) from west central Africa. SIVgor is closely related to chimpanzee and human immunodeficiency viruses (SIVcpz and HIV‐1, respectively). We established a noninvasive method that does not interfere with gorillas' natural behaviour to provide wildlife pathogen surveillance and health monitoring for conservation. A total of 1,665 geo‐referenced fecal samples were collected at regular intervals from February 2006 to December 2014 (123 sampling days) in the Campo‐Ma'an National Park (southwest Cameroon). Host genotyping was performed using microsatellite markers, SIVgor infection was identified by serology and genetic amplification was attempted on seropositive individuals. We identified at least 125 distinct gorillas, 50 were resampled (observed 3.5 times in average) and 38 were SIVgor+ (seven individuals were seroconverters). Six groups of gorillas were identified based on the overlapping occurrence of individuals with apparent high rates of gene flow. We obtained SIVgor genetic sequences from 25 of 38 seropositive genotyped gorillas and showed that the virus follows exponential growth dynamics under a strict molecular clock. Different groups shared SIVgor lineages demonstrating intergroup viral spread and recapture of positive individuals illustrated intra‐host viral evolution. Relatedness and relationship genetic analysis of gorillas together with Bayesian phylogenetic inference of SIVgor provided evidence suggestive of vertical transmission. In conclusion, we provided insights into gorilla social dynamics and SIVgor evolution and emphasized the utility of noninvasive sampling to study wildlife health populations. These findings contribute to prospective planning for better monitoring and conservation. |
format | Online Article Text |
id | pubmed-6262910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62629102018-12-05 Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon Villabona‐Arenas, Christian Julian Ayouba, Ahidjo Esteban, Amandine D'arc, Mirela Mpoudi Ngole, Eitel Peeters, Martine Ecol Evol Original Research Simian immunodeficiency virus (SIVgor) causes persistent infection in critically endangered western lowland gorillas (Gorilla gorilla gorilla) from west central Africa. SIVgor is closely related to chimpanzee and human immunodeficiency viruses (SIVcpz and HIV‐1, respectively). We established a noninvasive method that does not interfere with gorillas' natural behaviour to provide wildlife pathogen surveillance and health monitoring for conservation. A total of 1,665 geo‐referenced fecal samples were collected at regular intervals from February 2006 to December 2014 (123 sampling days) in the Campo‐Ma'an National Park (southwest Cameroon). Host genotyping was performed using microsatellite markers, SIVgor infection was identified by serology and genetic amplification was attempted on seropositive individuals. We identified at least 125 distinct gorillas, 50 were resampled (observed 3.5 times in average) and 38 were SIVgor+ (seven individuals were seroconverters). Six groups of gorillas were identified based on the overlapping occurrence of individuals with apparent high rates of gene flow. We obtained SIVgor genetic sequences from 25 of 38 seropositive genotyped gorillas and showed that the virus follows exponential growth dynamics under a strict molecular clock. Different groups shared SIVgor lineages demonstrating intergroup viral spread and recapture of positive individuals illustrated intra‐host viral evolution. Relatedness and relationship genetic analysis of gorillas together with Bayesian phylogenetic inference of SIVgor provided evidence suggestive of vertical transmission. In conclusion, we provided insights into gorilla social dynamics and SIVgor evolution and emphasized the utility of noninvasive sampling to study wildlife health populations. These findings contribute to prospective planning for better monitoring and conservation. John Wiley and Sons Inc. 2018-10-25 /pmc/articles/PMC6262910/ /pubmed/30519399 http://dx.doi.org/10.1002/ece3.4478 Text en © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Villabona‐Arenas, Christian Julian Ayouba, Ahidjo Esteban, Amandine D'arc, Mirela Mpoudi Ngole, Eitel Peeters, Martine Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon |
title | Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon |
title_full | Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon |
title_fullStr | Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon |
title_full_unstemmed | Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon |
title_short | Noninvasive western lowland gorilla's health monitoring: A decade of simian immunodeficiency virus surveillance in southern Cameroon |
title_sort | noninvasive western lowland gorilla's health monitoring: a decade of simian immunodeficiency virus surveillance in southern cameroon |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262910/ https://www.ncbi.nlm.nih.gov/pubmed/30519399 http://dx.doi.org/10.1002/ece3.4478 |
work_keys_str_mv | AT villabonaarenaschristianjulian noninvasivewesternlowlandgorillashealthmonitoringadecadeofsimianimmunodeficiencyvirussurveillanceinsoutherncameroon AT ayoubaahidjo noninvasivewesternlowlandgorillashealthmonitoringadecadeofsimianimmunodeficiencyvirussurveillanceinsoutherncameroon AT estebanamandine noninvasivewesternlowlandgorillashealthmonitoringadecadeofsimianimmunodeficiencyvirussurveillanceinsoutherncameroon AT darcmirela noninvasivewesternlowlandgorillashealthmonitoringadecadeofsimianimmunodeficiencyvirussurveillanceinsoutherncameroon AT mpoudingoleeitel noninvasivewesternlowlandgorillashealthmonitoringadecadeofsimianimmunodeficiencyvirussurveillanceinsoutherncameroon AT peetersmartine noninvasivewesternlowlandgorillashealthmonitoringadecadeofsimianimmunodeficiencyvirussurveillanceinsoutherncameroon |