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Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate
In animal prion diseases, including bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in cervids, and scrapie in sheep and goats, a disease-associated isoform of prion protein (PrP(d)) accumulates in the brains of affected animals. Although the CH1641 scrapie isolate was expe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262972/ https://www.ncbi.nlm.nih.gov/pubmed/30486902 http://dx.doi.org/10.1186/s13567-018-0611-1 |
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author | Miyazawa, Kohtaro Masujin, Kentaro Matsuura, Yuichi Iwamaru, Yoshifumi Yokoyama, Takashi Okada, Hiroyuki |
author_facet | Miyazawa, Kohtaro Masujin, Kentaro Matsuura, Yuichi Iwamaru, Yoshifumi Yokoyama, Takashi Okada, Hiroyuki |
author_sort | Miyazawa, Kohtaro |
collection | PubMed |
description | In animal prion diseases, including bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in cervids, and scrapie in sheep and goats, a disease-associated isoform of prion protein (PrP(d)) accumulates in the brains of affected animals. Although the CH1641 scrapie isolate was experimentally established in the UK, a few natural CH1641-like scrapie cases have been reported in France and the UK. The molecular mass of the unglycosylated protease-resistant core of PrP(d) (PrPres) is known to be similar between CH1641-like scrapie and experimental BSE in sheep. We previously established an experimental CH1641-like scrapie isolate (Sh294) from a natural classical scrapie case. Here, we demonstrated that the Sh294 isolate was independent of both classical and atypical BSEs by cross-species transmission to bovine PrP overexpressing (TgBoPrP) mice and wild-type mice. Interestingly, we found that the Sh294 isolate altered its host range by the transmission to TgBoPrP mice, and we succeeded in the first stable reproduction of CH1641-like scrapie specific PrPres banding patterns with the ~12-kDa small C-terminal fragment in wild-type mice. This study provides new insight into the relationship between CH1641-like scrapie isolates and BSEs. In addition, interspecies transmission models such as we have demonstrated here could be a great help to investigate the origin and host range of animal prions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0611-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6262972 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62629722018-12-10 Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate Miyazawa, Kohtaro Masujin, Kentaro Matsuura, Yuichi Iwamaru, Yoshifumi Yokoyama, Takashi Okada, Hiroyuki Vet Res Research Article In animal prion diseases, including bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in cervids, and scrapie in sheep and goats, a disease-associated isoform of prion protein (PrP(d)) accumulates in the brains of affected animals. Although the CH1641 scrapie isolate was experimentally established in the UK, a few natural CH1641-like scrapie cases have been reported in France and the UK. The molecular mass of the unglycosylated protease-resistant core of PrP(d) (PrPres) is known to be similar between CH1641-like scrapie and experimental BSE in sheep. We previously established an experimental CH1641-like scrapie isolate (Sh294) from a natural classical scrapie case. Here, we demonstrated that the Sh294 isolate was independent of both classical and atypical BSEs by cross-species transmission to bovine PrP overexpressing (TgBoPrP) mice and wild-type mice. Interestingly, we found that the Sh294 isolate altered its host range by the transmission to TgBoPrP mice, and we succeeded in the first stable reproduction of CH1641-like scrapie specific PrPres banding patterns with the ~12-kDa small C-terminal fragment in wild-type mice. This study provides new insight into the relationship between CH1641-like scrapie isolates and BSEs. In addition, interspecies transmission models such as we have demonstrated here could be a great help to investigate the origin and host range of animal prions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0611-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-28 2018 /pmc/articles/PMC6262972/ /pubmed/30486902 http://dx.doi.org/10.1186/s13567-018-0611-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Miyazawa, Kohtaro Masujin, Kentaro Matsuura, Yuichi Iwamaru, Yoshifumi Yokoyama, Takashi Okada, Hiroyuki Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate |
title | Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate |
title_full | Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate |
title_fullStr | Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate |
title_full_unstemmed | Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate |
title_short | Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate |
title_sort | interspecies transmission to bovinized transgenic mice uncovers new features of a ch1641-like scrapie isolate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262972/ https://www.ncbi.nlm.nih.gov/pubmed/30486902 http://dx.doi.org/10.1186/s13567-018-0611-1 |
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