Microcosm biofilms cultured from different oral niches in periodontitis patients

Objective: Periodontal diseases are triggered by dysbiotic microbial biofilms. Therefore, it is essential to develop appropriate biofilm models. Aim of the present study was to culture microcosm biofilms inoculated from different niches in periodontitis patients and compare their microbial compositi...

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Autores principales: Cieplik, Fabian, Zaura, Egija, Brandt, Bernd W., Buijs, Mark J., Buchalla, Wolfgang, Crielaard, Wim, Laine, Marja L., Deng, Dong Mei, Exterkate, Rob A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263112/
https://www.ncbi.nlm.nih.gov/pubmed/30598734
http://dx.doi.org/10.1080/20022727.2018.1551596
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author Cieplik, Fabian
Zaura, Egija
Brandt, Bernd W.
Buijs, Mark J.
Buchalla, Wolfgang
Crielaard, Wim
Laine, Marja L.
Deng, Dong Mei
Exterkate, Rob A. M.
author_facet Cieplik, Fabian
Zaura, Egija
Brandt, Bernd W.
Buijs, Mark J.
Buchalla, Wolfgang
Crielaard, Wim
Laine, Marja L.
Deng, Dong Mei
Exterkate, Rob A. M.
author_sort Cieplik, Fabian
collection PubMed
description Objective: Periodontal diseases are triggered by dysbiotic microbial biofilms. Therefore, it is essential to develop appropriate biofilm models. Aim of the present study was to culture microcosm biofilms inoculated from different niches in periodontitis patients and compare their microbial composition to those inoculated from subgingival plaque. Methods: Saliva, subgingival plaque, tongue and tonsils were sampled in five periodontitis patients to serve as inocula for culturing biofilms in vitro in an active attachment model. Biofilms were grown for 14 or 28 d and analyzed for their microbial composition by 16S rDNA sequencing. Results: As classified by HOMD, all biofilms were dominated by periodontitis-associated taxa, irrespective which niche had been used for inoculation. There was a low similarity between 14 d biofilms and their respective inocula (Bray-Curtis similarity 0.26), while biofilms cultured for 14 and 28 d shared high similarity (0.69). Principal components analysis showed much stronger clustering per patient than per niche indicating that the choice of patients may be more crucial than choice of the respective niches in these patients. Conclusion: Saliva, tongue scrapings or tonsil swabs may represent sufficient alternative inocula for growing microcosm biofilms resembling periodontitis-associated microbial communities in cases when sampling subgingival plaque is not possible.
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spelling pubmed-62631122019-01-01 Microcosm biofilms cultured from different oral niches in periodontitis patients Cieplik, Fabian Zaura, Egija Brandt, Bernd W. Buijs, Mark J. Buchalla, Wolfgang Crielaard, Wim Laine, Marja L. Deng, Dong Mei Exterkate, Rob A. M. J Oral Microbiol Original Article Objective: Periodontal diseases are triggered by dysbiotic microbial biofilms. Therefore, it is essential to develop appropriate biofilm models. Aim of the present study was to culture microcosm biofilms inoculated from different niches in periodontitis patients and compare their microbial composition to those inoculated from subgingival plaque. Methods: Saliva, subgingival plaque, tongue and tonsils were sampled in five periodontitis patients to serve as inocula for culturing biofilms in vitro in an active attachment model. Biofilms were grown for 14 or 28 d and analyzed for their microbial composition by 16S rDNA sequencing. Results: As classified by HOMD, all biofilms were dominated by periodontitis-associated taxa, irrespective which niche had been used for inoculation. There was a low similarity between 14 d biofilms and their respective inocula (Bray-Curtis similarity 0.26), while biofilms cultured for 14 and 28 d shared high similarity (0.69). Principal components analysis showed much stronger clustering per patient than per niche indicating that the choice of patients may be more crucial than choice of the respective niches in these patients. Conclusion: Saliva, tongue scrapings or tonsil swabs may represent sufficient alternative inocula for growing microcosm biofilms resembling periodontitis-associated microbial communities in cases when sampling subgingival plaque is not possible. Taylor & Francis 2018-11-27 /pmc/articles/PMC6263112/ /pubmed/30598734 http://dx.doi.org/10.1080/20022727.2018.1551596 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cieplik, Fabian
Zaura, Egija
Brandt, Bernd W.
Buijs, Mark J.
Buchalla, Wolfgang
Crielaard, Wim
Laine, Marja L.
Deng, Dong Mei
Exterkate, Rob A. M.
Microcosm biofilms cultured from different oral niches in periodontitis patients
title Microcosm biofilms cultured from different oral niches in periodontitis patients
title_full Microcosm biofilms cultured from different oral niches in periodontitis patients
title_fullStr Microcosm biofilms cultured from different oral niches in periodontitis patients
title_full_unstemmed Microcosm biofilms cultured from different oral niches in periodontitis patients
title_short Microcosm biofilms cultured from different oral niches in periodontitis patients
title_sort microcosm biofilms cultured from different oral niches in periodontitis patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263112/
https://www.ncbi.nlm.nih.gov/pubmed/30598734
http://dx.doi.org/10.1080/20022727.2018.1551596
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