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Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research

Given the growing number of diseases caused by emerging or endemic viruses, original strategies are urgently required: (1) for the identification of new drugs active against new viruses and (2) to deal with viral mutants in which resistance to existing antiviral molecules has been selected. In this...

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Detalles Bibliográficos
Autores principales: Castel, Guillaume, Chtéoui, Mohamed, Heyd, Bernadette, Tordo, Noël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263255/
https://www.ncbi.nlm.nih.gov/pubmed/21522083
http://dx.doi.org/10.3390/molecules16053499
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author Castel, Guillaume
Chtéoui, Mohamed
Heyd, Bernadette
Tordo, Noël
author_facet Castel, Guillaume
Chtéoui, Mohamed
Heyd, Bernadette
Tordo, Noël
author_sort Castel, Guillaume
collection PubMed
description Given the growing number of diseases caused by emerging or endemic viruses, original strategies are urgently required: (1) for the identification of new drugs active against new viruses and (2) to deal with viral mutants in which resistance to existing antiviral molecules has been selected. In this context, antiviral peptides constitute a promising area for disease prevention and treatment. The identification and development of these inhibitory peptides require the high-throughput screening of combinatorial libraries. Phage-display is a powerful technique for selecting unique molecules with selective affinity for a specific target from highly diverse combinatorial libraries. In the last 15 years, the use of this technique for antiviral purposes and for the isolation of candidate inhibitory peptides in drug discovery has been explored. We present here a review of the use of phage display in antiviral research and drug discovery, with a discussion of optimized strategies combining the strong screening potential of this technique with complementary rational approaches for identification of the best target. By combining such approaches, it should be possible to maximize the selection of molecules with strong antiviral potential.
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spelling pubmed-62632552018-12-10 Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research Castel, Guillaume Chtéoui, Mohamed Heyd, Bernadette Tordo, Noël Molecules Review Given the growing number of diseases caused by emerging or endemic viruses, original strategies are urgently required: (1) for the identification of new drugs active against new viruses and (2) to deal with viral mutants in which resistance to existing antiviral molecules has been selected. In this context, antiviral peptides constitute a promising area for disease prevention and treatment. The identification and development of these inhibitory peptides require the high-throughput screening of combinatorial libraries. Phage-display is a powerful technique for selecting unique molecules with selective affinity for a specific target from highly diverse combinatorial libraries. In the last 15 years, the use of this technique for antiviral purposes and for the isolation of candidate inhibitory peptides in drug discovery has been explored. We present here a review of the use of phage display in antiviral research and drug discovery, with a discussion of optimized strategies combining the strong screening potential of this technique with complementary rational approaches for identification of the best target. By combining such approaches, it should be possible to maximize the selection of molecules with strong antiviral potential. MDPI 2011-04-26 /pmc/articles/PMC6263255/ /pubmed/21522083 http://dx.doi.org/10.3390/molecules16053499 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Castel, Guillaume
Chtéoui, Mohamed
Heyd, Bernadette
Tordo, Noël
Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research
title Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research
title_full Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research
title_fullStr Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research
title_full_unstemmed Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research
title_short Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research
title_sort phage display of combinatorial peptide libraries: application to antiviral research
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263255/
https://www.ncbi.nlm.nih.gov/pubmed/21522083
http://dx.doi.org/10.3390/molecules16053499
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